A5022925792- Viral Infections and Immunology Research
- RNA regulation and disease
- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- Graphene and Nanomaterials Applications
- Oral microbiology and periodontitis research
- Autophagy in Disease and Therapy
- HER2/EGFR in Cancer Research
- Viral gastroenteritis research and epidemiology
- Cancer-related Molecular Pathways
- Electrospun Nanofibers in Biomedical Applications
- T-cell and Retrovirus Studies
- Herpesvirus Infections and Treatments
- Fungal Plant Pathogen Control
- HIV/AIDS oral health manifestations
- Scientific and Engineering Research Topics
- NF-κB Signaling Pathways
- Dental materials and restorations
- Inflammasome and immune disorders
Harbin Medical University
2014-2024
MicroRNAs (miRNAs) are small non-coding RNAs that can posttranscriptionally regulate gene expression by targeting messenger RNAs. During miRNA biogenesis, the star strand (miRNA*) is generally degraded to a low level in cells. However, certain miRNA* express abundantly and be recruited into silencing complex expression. Most miRNAs function as suppressive regulators on Group B coxsackieviruses (CVB) major pathogens of human viral myocarditis dilated cardiomyopathy. CVB genome positive-sense,...
Human enterovirus 71 (EV71) is the main causative pathogen of hand, foot, and mouth disease (HFMD) in children. The epidemic HFMD has been a public health problem Asia-Pacific region for decades, no vaccine effective antiviral medicine are available. Curcumin used as traditional centuries to treat diversity disorders including viral infections. In this study, we demonstrated that curcumin showed potent effect again EV71. Vero cells infected with EV71, addition significantly suppressed...
Myocarditis is an inflammatory disease of the cardiac muscle and one primary causes dilated cardiomyopathy. Group B coxsackievirus (CVB) leading causative pathogens viral myocarditis, which primarily affects children young adults. Due to lack vaccines, development antiviral medicines crucial controlling CVB infection progression myocarditis. In this study, we investigated effect baicalein, a flavonoid extracted from Scutellaria baicaleinsis. Our results demonstrated that baicalein treatment...
Objective. miR-122 is highly abundant in liver and a hepato-specific microRNA. There evidence to show that expression down-regulated human hepatocellular carcinoma (HCC). It not known whether affects the cellular behavior of hepatoma cells. The aim this study was investigate effects on viability apoptosis Material methods. Huh-7 HepG2 cells treated with or antisense RNA (anti-miR-122) were analyzed by adenosine triphosphate (ATP)-based luminescent assay, annexin V-based flow cytometry,...
Stress granules (SGs) are granular aggregates in the cytoplasm that formed under a variety of stress situations including viral infection. Previous studies indicate poliovirus, member Picornaviridae, can induce SG formation. However, exact mechanism by which picornaviruses formation is unknown. The localization markers cells infected with coxsackievirus B3 (CVB3) or enterovirus 71 (EV71) and expressing each protein was determined via immunofluorescence assays plasmid transfection. Eight...
Coxsackievirus B (CVB) is the major cause of human myocarditis and dilated cardiomyopathy. Toll-like receptor 3 (TLR3) an intracellular sensor to detect pathogen's dsRNA. TLR3, along with TRAF6, triggers inflammatory response through NF-κB signaling pathway. In cells infected CVB type (CVB3), abundance miR-146a was significantly increased. The role in infection unclear. this study, TLR3 TRAF6 were identified as targets miR-146a. elevated inhibited translocation subsequently down-regulated...
Coxsackievirus B (CVB) belongs to Enterovirus genus within the Picornaviridae family, and it is one of most common causative pathogens viral myocarditis in young adults. The pathogenesis caused by CVB has not been completely elucidated. In infection, autophagy manipulated facilitate replication. Here we report that protein 2B, non-structural proteins CVB3, possesses autophagy-inducing capability. motif 2B was identified generation truncated site-directed mutagenesis. expression alone...
Hepatocellular carcinoma (HCC) is one of the common cancers worldwide, especially in developing countries. Although chronic infections hepatitis B and C viruses have been established as etiological factors HCC, mechanism for tumorigenesis development HCC still unclear. The liver-specific microRNA-122 (miR-122), an tumor-suppressor miRNA, often down-regulated while underlying not well understood. Here we report that AU-rich element-binding factor AUF1 suppresses expression Dicer1, type III...
Coxsackievirus B (CVB) is the major causative pathogen for severe diseases such as viral myocarditis, meningitis, and pancreatitis. There no effective antiviral therapy currently available CVB infection primarily due to that pathogenesis of has not been completely understood. Viruses are obligate intracellular pathogens which subvert cellular processes ensure replication. Dysregulation ubiquitination implicated in infection. However, how involved remains unclear. Here we found 3D protein...
<i>Aims:</i> To evaluate the stability of coxsackievirus B (CVB) genome integrated with enhanced green fluorescent protein gene <i>(egfp)</i> and provide valuable information for use recombinant CVB variant. <i>Methods:</i> A CVB3 variant expressing eGFP was constructed by insertion <i>egfp</i> open-reading frame (ORF) at 5′ end ORF. The virus CVB3-eGFP serially passaged in HeLa cells. deletions around were examined reverse transcription...
Anti-inflammation and bone regeneration are the two major goals of periodontal therapy. We have demonstrated that chitosan (CS)/polyvinyl alcohol (PVA)/graphene oxide (GO)/astaxanthin (ASTA) nanofibers membranes prepared by electrospinning had favorable micro-morphology, good mechanical properties, no cytotoxicity. In this study, CS/PVA/GO/ASTA were to modulate both inflammatory response osteogenic induction in vitro study. When co-cultured with RAW264.7 cells, glycoprotein nonmetastatic...