A5102008964- Reproductive Biology and Fertility
- Reconstructive Facial Surgery Techniques
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Ovarian function and disorders
- Selenium in Biological Systems
- Peptidase Inhibition and Analysis
- Cardiomyopathy and Myosin Studies
- Ear and Head Tumors
- Congenital Ear and Nasal Anomalies
- Cancer-related molecular mechanisms research
- Viral Infections and Immunology Research
- Circadian rhythm and melatonin
- Congenital Heart Disease Studies
- Renal and related cancers
- Birth, Development, and Health
- Congenital heart defects research
- Cardiac Fibrosis and Remodeling
- Histone Deacetylase Inhibitors Research
- Reproductive Health and Technologies
- Cardiovascular Function and Risk Factors
- Signaling Pathways in Disease
- Ear Surgery and Otitis Media
- Reconstructive Surgery and Microvascular Techniques
Sichuan Agricultural University
2018-2025
Children's Hospital of Chongqing Medical University
2015-2025
Chongqing Medical University
2015-2025
China International Science and Technology Cooperation
2014-2025
Anhui Agricultural University
2024-2025
National Heart Lung and Blood Institute
2025
Ministry of Agriculture and Rural Affairs
2022-2025
National Institutes of Health
2025
Taizhou People's Hospital
2024
Yangzhou University
2024
Prenatal alcohol exposure may cause cardiac development defects, however, the underlying mechanisms are not yet clear. In present study we have investigated roles of histone modification by curcumin on induced fetal abnormalities during development. Q-PCR and Western blot results showed that increased gene active forms caspase-3 caspase-8, while decreased protein bcl-2. ChIP assay that, acetylation H3K9 near promoter region TUNEL data revealed apoptosis levels in embryonic hearts. vitro...
Bone marrow derived stem cells (BMSCs) have the potential to differentiate into cardiomyocytes, but rate of differentiation is low and mechanism unclear completely. Here, we aimed investigate role miR1-2 in mouse BMSCs cardiomyocyte-like reveal involved signaling pathways procedure.Mouse were treated with 5-azacytine (5-aza). The expression cardiac cell markers: NKx2.5, cTnI GATA4 examined by qPCR. apoptosis was detected flow cytometry activity Wnt/β-catenin pathway evaluated measuring...
Human enterovirus 71 (EV71) is the main causative pathogen of hand, foot, and mouth disease (HFMD) in children. The epidemic HFMD has been a public health problem Asia-Pacific region for decades, no vaccine effective antiviral medicine are available. Curcumin used as traditional centuries to treat diversity disorders including viral infections. In this study, we demonstrated that curcumin showed potent effect again EV71. Vero cells infected with EV71, addition significantly suppressed...
The present study aimed to investigate the effect of melatonin (MT) supplementation on in vitro maturation vitrified mouse germinal vesicle (GV) oocytes. fresh oocytes were randomly divided into three groups: untreated (control), or by open-pulled straw method without (vitrification group) with MT + group). After warming, cultured vitro, then reactive oxygen species (ROS) and glutathione (GSH) levels, mitochondrial membrane potential, ATP spindle morphology, mRNA expression assembly...
The motility of sperm decreases following cryopreservation, which is closely associated with mitochondrial function. However, the alterations in metabolism after freezing goats remain unclear. This experiment aimed to investigate impact ultra-low temperature on goat sperm's energy and its potential correlation motility. results revealed that exhibited vacuolization, reduced matrix density, significantly decreased levels high-membrane mitochondria adenosine triphosphate content, accompanied...
Objective: To investigate the disease burden, clinical characteristics and independent risk factors affecting in-hospital outcomes of children with congenital heart (CHD) combined failure (HF) in China. Methods: (1) Descriptive study: based on global burden study 2021, available data under 15 years age CHD HF China from 1990 to 2021 were collected. The prevalence trends different subgroups (<1 year, 1-<2 years, 2-<5 5-<10 10-<15 years) analyzed, annual percentage change (EAPC) was estimated...
The involvement of chemosensory proteins (CSPs) in binding to insecticides has been implicated. However, our understanding CSP-mediated insecticide resistance remains limited. Herein, 15 CSP genes were identified and characterized from Nilaparvata lugens. Expression analysis six CSPs with overexpression the imidacloprid-resistant strain, whose imidacloprid was validated by RNA interference. Among them, four successfully expressed using a prokaryotic expression system, their affinities...
Abstract Hybrid incompatibility can lead to lethality and sterility of F1 hybrids, contributing speciation. Here we found that female hybrids between Mus musculus domesticus spicilegus mice are sterile due the failure homologous chromosome separation in oocyte meiosis I, producing aneuploid eggs. This non-separation phenotype was driven by mis- localization cohesin protector, SGO2, along arms instead its typical centromeric enrichment, resulting over-protection. The upstream kinase, BUB1,...
Cardiac remodeling in response to disease or tissue damage severely impairs heart function. Therefore, the description of molecular mechanisms responsible is essential for development effective therapies. Trbp (Tarbp2) a multifunctional RNA-binding protein that during development, but its role adult and cardiac remains unknown. We generated inducible conditional knockout mice delete from cardiomyocytes young adults (Trbp-cKOs). While Trbp-cKO did not display detectable phenotype, under...
ABSTRACT The intricate mechanisms driving oocyte maturation remain only partially understood, especially within the domains of domestic animal reproduction and translational medicine. In case prepubertal girls, clinical challenge is pronounced, as ovarian tissue cryopreservation‐though promising‐remains an experimental technique necessitating rigorous scientific validation to guarantee developmental potential preserved materials facilitate broader adoption. To address these knowledge gaps,...
Abstract Cardiac diastolic dysfunction ( CDD ) is the most common form of cardiovascular disorders, especially in elderly people. troponin I cTnI plays a critical role regulation cardiac function, function. Our previous studies showed that ‐low expression induced by histone acetylation modification might be one causes result ageing hearts. This study was designed to investigate whether epigallocatechin‐3‐gallate EGCG would modify events regulate and then improve functions mice. shows...
This study was conducted to investigate the effect of vitrification on dynamics global transcriptome in bovine germinal vesicle (GV) oocytes and their vitro-derived metaphase II (MII) oocytes. The GV were vitrified using open-pulled straw method. After warming, resulting MII-stage cultured vitro for 2 h 24 respectively then collected. fresh MII used as controls. Then, each pool (fresh GV, n = 3; 4; MII, 1 derived from 2) different stages mRNA sequencing. results showed that maturation rates...
Abstract Ambient particles with a diameter of <2.5 μm (PM2.5) is global health concern, and exposure to PM2.5 contributes the progression cardiovascular morbidity mortality. In this study, pregnant c57 mice were exposed during whole gestation (approximately 300 µg/m 3 for 2 hours/d). A significantly low birth weight was found after in utero exposure, body continued 12 weeks birth. offspring, remarkable destructions cardiac ultrastructures determined both newborn adult hearts. adulthood,...
Our previous studies have demonstrated that Ca2+ desensitizing catechin could correct diastolic dysfunction in experimental animals with restrictive cardiomyopathy. In this study, it is aimed to assess the effects of green tea extract on cardiac function and other clinical features pediatric patients cardiomyopathies. Twelve cardiomyopathy were enrolled for study. Echocardiography, ECG, laboratory tests performed before after administration 12 months. Comparison has been made these treatment...
Female reproductive (ovarian) aging is distinctively characterized by a markedly reduced function due to remarkable decline in quality and quantity of follicles oocytes. Selenium (Se) has been implicated playing many important biological roles male fertility reproduction; however, its potential female reproduction, particularly subjects, remain poorly elucidated. Therefore, the current study we used murine model elucidated how different Se-levels might affect efficiency females. Our results...
Abstract Background This study investigated the effect of melatonin (MT) on cell cycle (G1/S/G2/M) parthenogenetic zygotes developed from vitrified-warmed mouse metaphase II (MII) oocytes and elucidated potential mechanism MT action in first cleavage embryos. Results After vitrification warming, were parthenogenetically activated (PA) vitro cultured (IVC). Then spindle morphology chromosome segregation oocytes, maternal mRNA levels genes including Miss, Doc1r, Setd2 Ythdf2 pronuclear...
Previously it was reported that melatonin could mitigate oxidative stress caused by oocyte cryopreservation; however, the underlying molecular mechanisms which cause this remain unclear. The objective to explore whether reduce during in vitro maturation of vitrified-warmed mouse germinal vesicle (GV) oocytes through Nrf2 signaling pathway or its receptors. During GV oocytes, there were decreases (p < 0.05) development rates metaphase I (MI) and II (MII) spindle morphology grades;...
Background Alcohol abuse during gestation may cause congenital heart diseases ( CHDs ). The underlying mechanisms of alcohol‐induced cardiac deformities are still not clear. Recent studies suggest that histone modification play a crucial role in this pathological process. Moreover, our previous reported ethanol could induce histone3 lysine9 (H3K9) hyperacetylation and overexpression development‐related genes vitro. aim study was to investigate the effect alcohol consumption on imbalance H 3...
Circulating tumor cells (CTCs) have been implicated in cancer prognosis and follow up. Detection of CTCs was considered significant evaluation. However, due to the heterogeneity rareness CTCs, detecting them with a single maker is usually challenged low specificity sensitivity. Previous studies concerning detection lung mainly focused on non-small cell carcinoma. Currently, there no report yet describing CTC multiple markers adenocarcinoma. In this study, by employing quantitative real-time...