A5001293412- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Ruminant Nutrition and Digestive Physiology
- Helminth infection and control
- Reproductive Physiology in Livestock
- Circadian rhythm and melatonin
- Genetic and phenotypic traits in livestock
- Animal Nutrition and Physiology
- Birth, Development, and Health
- Ovarian function and disorders
- Animal Genetics and Reproduction
- Selenium in Biological Systems
- Xenotransplantation and immune response
- Composting and Vermicomposting Techniques
- Meat and Animal Product Quality
- Rabbits: Nutrition, Reproduction, Health
- Coccidia and coccidiosis research
- Monoclonal and Polyclonal Antibodies Research
- Aluminum Alloy Microstructure Properties
- Pharmaceutical and Antibiotic Environmental Impacts
- Genetics, Aging, and Longevity in Model Organisms
- Hydrogen embrittlement and corrosion behaviors in metals
- Fungal Biology and Applications
- Aluminum Alloys Composites Properties
- Protein purification and stability
Jilin Agricultural University
2023-2025
Johnson & Johnson (United States)
2025
Ministry of Agriculture and Rural Affairs
2025
Sun Yat-sen University
2024
Northeast Institute of Geography and Agroecology
2015-2023
Chinese Academy of Sciences
2015-2023
Janssen (United States)
2014-2023
Second Hospital of Shanxi Medical University
2021
Shanxi Medical University
2021
China University of Petroleum, East China
2020
Structural and functional damages to mitochondria of frozen-thawed sperm are a typical cryoinjury, with mitochondrial permeability transition pore (MPTP) formation being the hallmark change. Mitochondria both primary synthesis site principle target for melatonin; this compound can directly inhibit MPTP therefore confer protection at level. The objective was determine effects melatonin on opening, viability, motility, oxidative phosphorylation (OXPHOS) ram sperm. Ram semen diluted in...
A number of classes proteins have been engineered for high stability using consensus sequence design methods. Here we describe the engineering a novel albumin binding domain (ABD) three-helix bundle protein. The resulting ABD molecule, called ABDCon, is expressed at levels in soluble fraction Escherichia coli and highly stable, with melting temperature 81.5°C. ABDCon binds human, monkey mouse serum albumins affinity as 61 pM. solution structure consistent epitope mapping studies enabled...
Alternative scaffold molecules represent a class of proteins important to the study protein design and mechanisms protein-protein interactions, as well for development therapeutic proteins. Here, we describe generation library built upon framework consensus FN3 domain sequence resulting in binding call Centyrins. This new employs diversified positions within C-strand, CD-loop, F-strand FG-loop domain. CIS display was used select high-affinity Centyrin variants against three targets; c-MET,...
The exfoliation corrosion behaviour of 2024 aluminium alloy was investigated through test, together with synchrotron radiation X-ray computed tomography (SR-CT) to achieve the 3D segmentation precipitated phases, cracks and products. results demonstrate that aging treatment has a significant effect on distribution phases in 2024-T4 alloy, continuously distributed along grain boundary would lead intergranular (IGC) which is easily evolved into corrosion. In 2024-T2 longest crack found locate...
Single-chain fragment variable (scFv) domains play an important role in antibody-based therapeutic modalities, such as bispecifics, multispecifics and chimeric antigen receptor T cells or natural killer cells. However, scFv exhibit lower stability increased risk of aggregation due to transient dissociation ("breathing") inter-molecular reassociation the two (VL VH). We designed a novel strategy, referred stapling, that introduces disulfide bonds between linker minimize breathing. named...
Microplastics (MPs), recognized as an emerging environmental menace, have been extensively investigated in both marine and terrestrial fauna. This study is comprehensive to investigate how polystyrene (PS) affects ruminant animals. The experimental design comprised 24 individually housed lambs, divided into a CON group (diet without PS) three PS-exposed (25 μm, 50 100 μm) groups, each with six the exposure of PS was mg/day, duration 60 days. yielded noteworthy results: (ⅰ) leads decrease...
The motility of sperm decreases following cryopreservation, which is closely associated with mitochondrial function. However, the alterations in metabolism after freezing goats remain unclear. This experiment aimed to investigate impact ultra-low temperature on goat sperm's energy and its potential correlation motility. results revealed that exhibited vacuolization, reduced matrix density, significantly decreased levels high-membrane mitochondria adenosine triphosphate content, accompanied...
T cell-redirecting bispecific antibodies (bsAbs) to treat advanced stage solid tumors are gaining interest after recent clinical successes. The immune checkpoint human leukocyte antigen G (HLA-G) is expressed in several tumor types while normal tissues expression limited. Here, we describe JNJ-78306358, a antibody (bsAb) tumors. JNJ-78306358 binds with high affinity the α3 subunit of HLA-G on cancer cells and purposely engineered weaker CD3ε cells. induced potent cell-mediated cytotoxicity...
Haemonchus contortus (H. contortus) infection has a significant impact on the health of pregnant ewes and adversely affects fetal development, highlighting critical need for non-toxic feed additive as an alternative sustainable control strategy. Tannin is kind polyphenol compound, which certain antiparasitic. The objective this study was to evaluate dietary tannin supplementation fecal egg count (FEC), packed cell volume (PCV), complete blood (CBC), hormone levels, inflammatory markers,...
The necroptosis of granulosa cells has been proven to be one the important triggers follicular atresia, which is an cause reduced reproductive capacity in cows. rapid growth accompanied by endoplasmic reticulum stress (ERS), leading cell death. However, link between ERS and necroptosis, as well its mechanism bovine still unclear. Itaconic acid endogenous anti-inflammatory antioxidant small-molecule compound that can alleviate ERS. Therefore, aim current study evaluate effect on investigate...
Periplaneta americana cockroach is an important source of inhalant indoor allergen resource, and there are more than twenty IgE-binding components identified in P. americana, but only nine allergens were characterized. Our knowledge about remains poor. In this work, two novel proteins Per a 11 (alpha-amylase) 12 (chitinase) with molecular weight around 55 45 kDa, respectively, purified characterized from the midgut cockroaches. Their primary sequences determined by Edman degradation, mass...
For the study, forty lambs were weighed and assigned into two treatments to determine effects of feeding garlic powder on growth performance, rumen fermentation, health status infected with gastrointestinal nematodes (GINs). The fed a basal diet without or 50 g/kg for 84 d. Data analyzed by general linear mixed model SAS software differences considered statistically significant if p ≤ 0.05. Results showed that supplementation increased lambs' average daily gain (p = 0.025), digestibility dry...
Abstract Cryopreservation damages permeability of sperm mitochondrial membranes, with formation a transition pore (mPTP). Mitochondria are both primary synthesis site and principle target for melatonin, which can directly inhibit mPTP formation. The objective was to determine effects melatonin on opening frozen‐thawed ram elucidate underlying pathways by antagonist agonists receptors (MTs), antagonists PI3K GSK 3β treatments; furthermore, plasma membrane integrity, potential (ΔΨm),...