A5074150995- Mechanical Circulatory Support Devices
- Cinema and Media Studies
- Cardiac Structural Anomalies and Repair
- Photography and Visual Culture
- Art, Politics, and Modernism
- Visual Culture and Art Theory
- Cardiac Valve Diseases and Treatments
- Italian Fascism and Post-war Society
- Cardiac and Coronary Surgery Techniques
- Cardiac pacing and defibrillation studies
- Art History and Market Analysis
- Heart Failure Treatment and Management
- Monoclonal and Polyclonal Antibodies Research
- Cardiovascular Function and Risk Factors
- Surgical Simulation and Training
- Cardiac, Anesthesia and Surgical Outcomes
- Architecture and Art History Studies
- Financial Crisis of the 21st Century
- Cardiac Arrest and Resuscitation
- Architecture, Modernity, and Design
- Infective Endocarditis Diagnosis and Management
- Aortic Disease and Treatment Approaches
- Shakespeare, Adaptation, and Literary Criticism
- Congenital Heart Disease Studies
- Growth Hormone and Insulin-like Growth Factors
Johnson & Johnson (United States)
2012-2025
Deutsches Herzzentrum der Charité
2002-2025
Charité - Universitätsmedizin Berlin
2025
KU Leuven
2015-2024
Medtronic (Netherlands)
2013-2024
Universitair Ziekenhuis Leuven
2017-2024
Geisinger Medical Center
2020-2024
Rivers State University
2024
The University of Queensland
2024
Janssen (United States)
2013-2023
Three monoclonal antibodies, designated alpha IR-1, IR-2, and IR-3, were prepared by fusing FO myeloma cells with spleen from a mouse immunized partially purified preparation of insulin receptors human placenta. These antibodies characterized their ability to immunoprecipitate solubilized labeled 125I-insulin or 125I-somatomedin-C in the presence absence various concentrations unlabeled somatomedin-C. IR-1 preferentially immunoprecipitates also less effectively somatomedin-C receptors, while...
Phosphorylation of the somatomedin-C receptor was investigated both in intact IM-9 cells and that had been solubilized with Triton X-100. Intact were incubated [32P]H3PO4 for 1 h an additional 5 min absence or presence insulin somatomedin-C. The then subjected to wheat germ agglutinin Sepharose chromatography. extent phosphorylation receptors assessed by immunoprecipitating eluates monoclonal antibodies specific each analyzing immunoprecipitates sodium dodecyl sulfate-polyacrylamide gel...
Tumor necrosis factor (TNF) is a proteinaceous toxin shed by stimulated myeloid cells. Murine TNF was radioiodinated to specific activity of 1 mCi/nmol (1 Ci = 37 GBq) monomer. 125I-labeled (125I-TNF) retained complete cytotoxic and it immunochemically identical the native in quantitative immunoprecipitation assay. It could be shown competition binding that 125I-TNF bound intact L929 cells with specificity equal toxin. The conditions time, temperature, concentration involved equilibrium were...
In both NIH3T3 cells and HepG2 cells, insulin-like growth factor I (IGF-I) receptors possess two @-subunits that display different electrophoretic mobilities.Increasing concentrations of IGF-I stimulated the phosphorylation &subunits to a similar extent, whereas insulin subunits only at elevated concentrations.Both were immunoprecipitated with p5, an receptor-specific anti-peptide antibody, or A410, polyclonal anti-insulin receptor antisera.However, if tetrameric was first dissociated into...
Antibodies to insulin receptors purified from rat liver membranes do not complete with [ 125 I]insulin for binding the receptor but precipitate solubilized labeled I]insulin. These antibodies have insulin-like activities of enhancing glucose oxidation and inhibiting epinephrine-induced lipolysis in adipocytes. Thus, antibody binds at a different site that which binds, yet interaction can initiate an effective biological response. results indicate previously studied insulin-binding sites are...
Human placental insulin receptorwas purified 11,000-fold to near homogeneity using DEAE-cellulose chromatography and affinity on insulin-Sepharose.Approximately 200 300 pg of receptor were obtained from a single placenta.In solution, the native is complex (Mr = 440,000) an acidic, multi-subunit protein with M, 350,000 bound detergent accounting for remainder mass.The asymmetric ( f / o 1.4) consists Coomassie blue staining polypeptide 135,000.In addition 135,000dalton polypeptide, two...
Cultured human lymphoid (IM-9) cells were labeled with [(35)S]methionine in the presence and absence of monensin, a carboxylic ionophore that inhibits post-translational protein maturation. Labeled receptors for insulin somatomedin C immunoprecipitated antibodies specific each receptor. Monensin biosynthesis mature alpha beta subunits both leads to accumulation immunoreactive polypeptides molecular weights 180,000. These 180,000 weight exist as disulfide-linked dimers may be biosynthetic...
Some antibodies have a tendency to self-associate leading precipitation at relatively low concentrations. CNTO607, monoclonal antibody, precipitates irreversibly in phosphate-buffered saline concentrations above 13 mg/ml. Previous mutagenesis work based on the Fab crystal structure pinpointed three residue fragment heavy chain CDR-3, (99)FHW(100a), as an aggregation epitope that is anchored by two salt bridges. Biophysical characterization of variants reveals F99 and W100a, but not H100,...
No abstract available.
List of Illustrations Preface Acknowledgements 1. Camera and Canvas: Emmer, Storck, Resnais the Post-War Art Film 2. Vasari in Hollywood: Biopics Artists 3. Galleries Gaze: Museums Rossellini's Viaggio Italia Hitchcock's Vertigo 4. Tableaux Vivants 1: Painting, Film, Death Passion Plays Pasolini Godard 5. 2: Stills Contemporary Photography 6. The Video That Knew Too Much: Hitchcock, Post-Cinema Appendix Bibliography Index
A number of classes proteins have been engineered for high stability using consensus sequence design methods. Here we describe the engineering a novel albumin binding domain (ABD) three-helix bundle protein. The resulting ABD molecule, called ABDCon, is expressed at levels in soluble fraction Escherichia coli and highly stable, with melting temperature 81.5°C. ABDCon binds human, monkey mouse serum albumins affinity as 61 pM. solution structure consistent epitope mapping studies enabled...
Targeted delivery of therapeutic payloads to specific tissues and cell types is an important component modern pharmaceutical development. Antibodies or other scaffold proteins can provide the cellular address for delivering a covalently linked via binding cell-surface receptors. Optimization conjugation site on targeting protein, linker chemistry intracellular trafficking pathways all influence efficiency potency drug candidate. In this study, we describe comprehensive engineering experiment...
Single-chain fragment variable (scFv) domains play an important role in antibody-based therapeutic modalities, such as bispecifics, multispecifics and chimeric antigen receptor T cells or natural killer cells. However, scFv exhibit lower stability increased risk of aggregation due to transient dissociation ("breathing") inter-molecular reassociation the two (VL VH). We designed a novel strategy, referred stapling, that introduces disulfide bonds between linker minimize breathing. named...
Solution-based affinity assays are used for the selection and characterization of proteins that could be developed into therapeutic molecules. However, these have limitations cell-surface as in most cases their purification requires detergent solubilization unlikely to assume conformations solution resemble native states cell membranes. This report describes a novel electrochemiluminescence-based method, called MSD-CAT, analysis antibodies binding receptors. MSD-CAT was evaluate monoclonal...