A5029852970- Monoclonal and Polyclonal Antibodies Research
- CAR-T cell therapy research
- Protein purification and stability
- Developmental Biology and Gene Regulation
- T-cell and B-cell Immunology
- NF-κB Signaling Pathways
- Glycosylation and Glycoproteins Research
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Biosimilars and Bioanalytical Methods
- Curcumin's Biomedical Applications
- Microfluidic and Capillary Electrophoresis Applications
- Protein Tyrosine Phosphatases
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Viral Infectious Diseases and Gene Expression in Insects
- Marine Ecology and Invasive Species
- Proteoglycans and glycosaminoglycans research
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
Indo Soviet Friendship College of Pharmacy
2025
Johnson & Johnson (United States)
2020-2025
Janssen (United States)
2017-2022
Springhouse
2022
Banaras Hindu University
1991-2022
Boehringer Ingelheim (United States)
2015-2020
Institute of Chemical Technology
2015
Amgen (United States)
2015
Hexal (Germany)
2015
University of New Hampshire
2015
When activated, NF-κB, a ubiquitous transcription factor, binds DNA as heterodimeric complex composed of members the Rel/NF-κB family polypeptides. Because its intimate involvement in host defense against disease, this factor is an important target for therapeutic intervention. In present report we demonstrate that curcumin (diferuloylmethane), known anti-inflammatory and anticarcinogenic agent, potent inhibitor NF-κB activation. Treatment human myeloid ML-1a cells with tumor necrosis (TNF)...
The p60 form of the tumor necrosis factor (TNF) receptor lacks motifs characteristic tyrosine or serine/threonine protein kinases. Our recent observations have indicated that a TNF receptor-associated kinase (p60-TRAK) from U-937 cells physically interacts with and causes phosphorylation cytoplasmic domain receptor. To define which region is necessary for physical interaction p60-TRAK, we constructed series deletions (grouped into three sets Δ1-Δ5, Δ6-Δ12, Δ13-Δ16) domain, expressed them as...
The Notch pathway regulates the development of many tissues and cell types is involved in a variety human diseases, making it an attractive potential therapeutic target. This promise has been limited by absence potent inhibitors or agonists that are specific for individual receptors (NOTCH1-4). Using unbiased functional screening, we identified monoclonal antibodies specifically inhibit induce activating proteolytic cleavages NOTCH3. Remarkably, most inhibitory bind to overlapping epitopes...
Herein, we describe the generation and characterization of BI 655066, a novel, highly potent neutralizing anti-interleukin-23 (IL23) monoclonal antibody in clinical development for autoimmune conditions, including psoriasis Crohn's disease. IL23 is key driver differentiation, maintenance, activity number immune cell subsets, T helper 17 (Th17) cells, which are believed to mediate pathogenesis several immune-mediated disorders. Thus, neutralization an attractive therapeutic approach....
Practically, IgG charge can contribute significantly to thermodynamic nonideality, and hence solubility viscosity. Biologically, isomers exhibit differences in clearance potency. It has been known since the 1930s that all immunoglobulins carry a weak negative physiological solvents. However, there no systematic exploration of this fundamental property. Accurate measurements have made using membrane confined electrophoresis two solvents (pH 5.0 pH 7.4) on panel twelve mAb IgGs, as well their...
Recent cloning of the cDNA for Fas/Apo‐1 and its ligand has revealed that they belong to tumor necrosis factor (TNF) receptor TNF family, respectively, play an important role in apoptosis (programmed cell death). Like TNF, antibodies against Fas antigen (anti‐Fas) have been shown be cytotoxic Fas‐expressing cells. Whether Fas, like receptor, also mediates proliferation normal human diploid fibroblasts (HDF), is not known. In this study, we show HDF expresses engagement signals these cells a...
Deficiency of interleukin (IL)-36 receptor antagonist (DITRA) syndrome is a rare autosomal recessive disease caused by mutations in IL36RN. IL-36R cell surface and member the IL1R family that involved inflammatory responses triggered skin other epithelial tissues. Accumulating evidence suggests signaling may play role pathogenesis psoriasis. Therapeutic intervention offers an innovative treatment paradigm for targeting cell-mediated diseases such as life-threatening psoriasis variant called...
Aggregation is mediated by local unfolding to allow aggregation “hot spot(s)” become solvent exposed and available associate with a hot spot on another partially unfolded protein. Historically, the of either crystallizable fragment (Fc) or antigen binding (Fab) regions given monoclonal antibody (MAb) has been implicated in aggregation, differing results across different proteins. The present work focuses separately quantifying kinetics isolated Fc, Fab, intact MAb as function pH under...
Abstract Despite significant progress over the last few decades in treatment of acute myeloid leukemia (AML), there still remains a major unmet medical need for this disease. Immunotherapy approaches redirecting pan CD3 + T cells to target blasts have shown limited efficacy clinical trials and often accompanied with severe toxicity AML patients. We designed an alternative engager molecule (Anti-TRGV9/anti-CD123), bispecific antibody that can simultaneously bind Vγ9 chain Vγ9Vδ2 γδ cell...
The near impermeability of the blood-brain barrier (BBB) and unique neuroimmune environment CNS prevents effective use antibodies in neurological diseases. Delivery biotherapeutics to brain can be enabled through receptor-mediated transcytosis via proteins such as transferrin receptor, although limitations ability Fc-mediated effector function clear pathogenic targets introduce safety liabilities. Hence, novel delivery approaches with alternative clearance mechanisms are warranted.Binders...
The transcription factor NF-kappaB is retained in the cytoplasm by its interaction with inhibitory subunit known as IkappaB. Signal-induced serine phosphorylation and subsequent ubiquitination of IkappaBalpha target it for degradation 26 S proteasome. Recently, pervanadate, a protein-tyrosine phosphatase inhibitor, was shown to block IkappaBalpha, thus inhibiting activation. We investigated mechanism which pervanadate inhibits IkappaBalpha. Western blot analysis from tumor necrosis...
Vol. 270, p. 24995 “Diferuloylmethane” was misspelled in the title. The corrected title is shown above.
T cell-redirecting bispecific antibodies (bsAbs) to treat advanced stage solid tumors are gaining interest after recent clinical successes. The immune checkpoint human leukocyte antigen G (HLA-G) is expressed in several tumor types while normal tissues expression limited. Here, we describe JNJ-78306358, a antibody (bsAb) tumors. JNJ-78306358 binds with high affinity the α3 subunit of HLA-G on cancer cells and purposely engineered weaker CD3ε cells. induced potent cell-mediated cytotoxicity...
PAX6 is a transcription activator that regulates eye development in animals ranging from Drosophila to human. The C-terminal region of isproline/serine/threonine-rich (PST) and functions as potent transactivation domain when attached heterologous DNA-binding the yeast factor, GAL4. PST comprises 152 amino acids encoded by four exons. function has not been defined characterized detail vitro mutagenesis. We dissected two independent systems, system using GAL4 site native PAX6. Our data...
PAX6 is a transcription factor with two DNA-binding domains (paired box and homeobox) proline-serine-threonine (PST)-rich transactivation domain. regulates eye development in animals ranging from jellyfish toDrosophila to humans. Heterozygous mutations the humanPAX6 gene result various phenotypes, including aniridia, Peter's anomaly, autosomal dominant keratitis, familial foveal dysplasia. It believed that mutated allele ofPAX6 produces an inactive protein aniridia caused due genetic...