A5018992850- Adipose Tissue and Metabolism
- Fatty Acid Research and Health
- Adipokines, Inflammation, and Metabolic Diseases
- Diet and metabolism studies
- Muscle metabolism and nutrition
- RNA Research and Splicing
- Cardiovascular Disease and Adiposity
- Curcumin's Biomedical Applications
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- HER2/EGFR in Cancer Research
- Cancer, Hypoxia, and Metabolism
- Gene expression and cancer classification
- Biochemical effects in animals
- Geological and Geochemical Analysis
- MicroRNA in disease regulation
- Liver Disease Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Geochemistry and Geologic Mapping
- Apelin-related biomedical research
- Birth, Development, and Health
- 14-3-3 protein interactions
- Metabolomics and Mass Spectrometry Studies
- Peroxisome Proliferator-Activated Receptors
Texas Tech University
2015-2024
Washington State University
2023
The University of Texas Southwestern Medical Center
2003-2017
Southwestern Medical Center
2011
Metal Oxide Technologies (United States)
2008
Anderson Hospital
1986
The Notch pathway regulates the development of many tissues and cell types is involved in a variety human diseases, making it an attractive potential therapeutic target. This promise has been limited by absence potent inhibitors or agonists that are specific for individual receptors (NOTCH1-4). Using unbiased functional screening, we identified monoclonal antibodies specifically inhibit induce activating proteolytic cleavages NOTCH3. Remarkably, most inhibitory bind to overlapping epitopes...
Abstract The epidermal growth factor receptor (EGFR) gene is commonly amplified and rearranged in glioblastoma multiforme leading to overexpression of wild-type mutant EGFRs. Expression EGFR ligands, such as transforming factor-α (TGF-α) or heparin-binding EGF (HB-EGF), also often increased gliomas resulting an autocrine loop that contributes the autonomy glioma cells. Glioblastoma multiformes express a characteristic (EGFRvIII, de 2-7) does not bind ligand, signals constitutively, more...
Brown adipose tissue (BAT) plays a key role in energy expenditure through its specialized thermogenic function. Therefore, BAT activation may help prevent and/or treat obesity. Interestingly, subcutaneous white (WAT) also has the ability to differentiate into brown-like adipocytes and potentially contribute increased thermogenesis. We have previously reported that eicosapentaenoic acid (EPA) reduces high-fat (HF)-diet-induced obesity insulin resistance mice. Whether mediates some of these...
Obesity prevalence continues to increase and contribute metabolic diseases, potentially by driving systemic inflammation. Curcumin is an anti-inflammatory spice with claimed health benefits. However, mechanisms which curcumin may reduce obesity-associated inflammation are poorly understood; thus, it hypothesized that benefits of consumption occur through reduced white adipose tissue (WAT) and/or beneficial changes in gut bacteria.Male B6 mice fed high-fat diets (HFD, 45% kcal fat) or HFD...
The Renin Angiotensin System (RAS), a key regulator of blood pressure has been linked to metabolic disorders. We have previously reported that adipose overexpression angiotensinogen in mice (Agt-Tg) induces obesity, part mediated by tissue inflammation, through yet unidentified mechanisms. Hence, we hypothesize enrichment leads activation inflammatory cascades and endoplasmic reticulum (ER) stress, thereby, contributing obesity. used wild type (Wt), Agt-Tg Agt-knockout (KO) along with 3T3-L1...
Jumonji (JmjC) domain proteins influence gene expression and chromatin organization by way of histone demethylation, which provides a means to regulate the activity genes across genome. JmjC have been associated with many human diseases including various cancers, developmental neurological disorders, however, shared biology possible common contribution organismal development tissue homeostasis all remains unclear. Here, we systematically tested function 13 Drosophila genes. Generation...
Scope Brown adipose tissue (BAT) dissipates energy through uncoupling protein 1 (UCP1) and has been proposed as an anti‐obesity target. It was reported previously that a high‐fat (HF) diet enriched in eicosapentaenoic acid (EPA) significantly increased UCP1 other thermogenic markers BAT. is hypothesized these effects are mediated UCP1‐dependent regulation. Methods results Wild‐type (WT) knockout (KO) B6 male mice were housed at thermoneutrality fed HF diet, without or with (EPA)‐enriched...
Obesity increases adipose tissue inflammation and secretion of pro-inflammatory adipokines, which have systemic effects on the organism’s health status. Our objective was to dissect mechanisms anti-inflammatory tart cherry (TC) in Zucker fatty rats, cultured 3T3-L1 adipocytes. Rats were fed either a control diet, or 4% TC powder diets for eight weeks. Body epididymal fat pad weights not significantly different between groups. However, rats diet had reduced (p < 0.05), as determined by...
Obesity is a complex disease of global epidemic proportions. Adipose tissue expansion and chronic low-grade inflammation, locally systemically, are hallmark features obesity. associated with several other diseases, which also characterized by inflammation. Determination adipocyte size macrophage content in adipose critical step assessing changes this Here, we introduce complete standalone software package, AdipoGauge, to analyse microscopic images derived from haematoxylin eosin...
Objective Over half of American women childbearing age have either obesity or overweight. Hence, maternal programming through diet is critical for prevention diseases in the offspring. Clinical trials with fish oil (FO) report various health benefits; however, it remains unclear whether and postnatal consumption FO protects offspring from adverse effects consuming a high‐fat (HF) diet. Methods Female mice were fed HF diets supplemented without 8 weeks before pregnancy lactation. A low‐fat...
Uncoupling protein 1 (UCP1) plays a central role in thermogenic tissues by uncoupling cellular respiration to dissipate energy. Beige adipocytes, an inducible form of cells subcutaneous adipose tissue (SAT), have become major focus obesity research. We previously shown that eicosapentaenoic acid (EPA) ameliorated high-fat diet (HFD)-induced activating brown fat C57BL/6J (B6) mice at thermoneutrality (30 °C), independently UCP1. Here, we investigated whether ambient temperature (22 °C)...
The plant-derived polyphenol curcumin alleviates the inflammatory and metabolic effects of obesity, in part, by reducing adipose tissue inflammation. We hypothesized that benefits supplementation on diet-induced obesity systemic inflammation mice occur through downregulation white (WAT) hypothesis was tested from high-fat obese supplemented with or without 3T3-L1 adipocytes treated curcumin. Male B6 were fed a diet (HFD, 45% kcal fat) 0.4% (w/w) (HFC). Metabolic changes these have been...
Worldwide rates of Western-diet-induced obesity epidemics are growing dramatically. Being linked with numerous comorbidities and complications, including cardiovascular disease, type 2 diabetes, cancer, chronic inflammation, osteoarthritis (OA), represents one the most threatening challenges for modern healthcare. Mouse models an invaluable tool investigating effects diets their bioactive components against high fat diet (HFD)-induced its comorbidities. During recent years, very (VHFDs),...
Obesity is a widespread epidemic that increases the risk for several metabolic diseases. Despite beneficial health effects of eicosapentaenoic acid (C20:5n-3, EPA), previous studies have used very high doses EPA. In this study, dose-dependent EPA on outcomes were determined in diet-induced obese mice. We B6 male mice, fed high-fat diet (HF, 45% kcal fat) or HF supplemented with 9, 18, and 36 g/kg EPA-enriched fish oil 14 weeks. conducted phenotyping during feeding period, harvested tissues...
Overactivation of the renin–angiotensin system (RAS) during obesity disrupts adipocyte metabolic homeostasis and induces endoplasmic reticulum (ER) stress inflammation; however, underlying mechanisms are not well known. We propose that overexpression angiotensinogen (Agt), precursor protein RAS in adipose tissue or treatment adipocytes with Angiotensin II (Ang II), bioactive hormone, alters specific microRNAs (miRNA), target ER inflammation leading to dysfunction. Epididymal white (WAT) from...
Conserved from yeast to humans, the Paf1 complex participates in a number of diverse processes including transcriptional initiation and polyadenylation. This typically includes five proteins: Paf1, Rtf1, Cdc73, Leo1, Ctr9. Previous efforts identified clear Drosophila homologs Cdc73 based on sequence similarity. Further work showed that these proteins help regulate gene expression are required for viability. To date, homolog Ctr9 has remained uncharacterized. Here, we show CG2469 encodes...