A5038983761- Neurobiology and Insect Physiology Research
- Circadian rhythm and melatonin
- Physiological and biochemical adaptations
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Chromatin Dynamics
- Zebrafish Biomedical Research Applications
- Insect and Pesticide Research
- Insect Utilization and Effects
- CRISPR and Genetic Engineering
- Light effects on plants
- Chromosomal and Genetic Variations
- Insect and Arachnid Ecology and Behavior
- Insect behavior and control techniques
- Photoreceptor and optogenetics research
- Epigenetics and DNA Methylation
- Neurotransmitter Receptor Influence on Behavior
- Animal Behavior and Reproduction
- Cellular transport and secretion
- Genetic Associations and Epidemiology
- Sleep and Wakefulness Research
- Retinal Development and Disorders
- Plant biochemistry and biosynthesis
- Lipid metabolism and biosynthesis
- Receptor Mechanisms and Signaling
- Cancer-related gene regulation
Huntsman Cancer Institute
2021-2025
University of Utah
2017-2025
The University of Texas Southwestern Medical Center
2011-2019
Southwestern Medical Center
2011-2019
Institute of Human Genetics
2019
Institute of Molecular Medicine
2018
Indiana University School of Medicine
2018
University of California, San Francisco
2002-2006
Rockefeller University
1996-2001
U.S. National Science Foundation
1996-2000
Two genes, period ( per ) and timeless tim ), are required for production of circadian rhythms in Drosophila . The proteins encoded by these genes (PER TIM) physically interact, the timing their association nuclear localization is believed to promote cycles transcription through an autoregulatory feedback loop. Here it shown that TIM protein may also couple this molecular pacemaker environment, because rapidly degraded after exposure light. accumulated rhythmically nuclei eyes cells brain....
The clock gene timeless ( tim ) is required for circadian rhythmicity in Drosophila . accumulation of RNA followed a rhythm, and the phase period rhythm were indistinguishable from those that have been reported per oscillations found to be dependent on presence PER TIM proteins, which demonstrates feedback control by mechanism previously shown regulate expression. cyclic expression appears dictate timing protein nuclear localization, suggesting promotes rhythms transcription restricting...
Significance We characterize in humans a coordinated network of brain activity describing neurobehavioral correlates reward anticipation. The involves nodes striatal and cortical regions, which are preferentially associated with distinct externalizing behaviors—hyperactivity alcohol consumption—suggesting that the heterogeneity reward-related behaviors might be accounted for by different association patterns their connecting links. In genome-wide study node subsequent functional validation...
Tolerance occurs when, following an initial experience with a substance, more of the substance is required subsequently to induce identical behavioral effects. not well-understood, and numerous researchers have turned model organisms, particularly Drosophila melanogaster, unravel its mechanisms. Flies high translational relevance for human alcohol responses, there substantial overlap in disease-causing genes between flies humans, including those associated Alcohol Use Disorder. Numerous...
Cyclophilin is an abundant and ubiquitous cytosolic protein that conserved throughout evolution from man to bacteria. It the target of immunosuppressive drug cyclosporin A. has peptidyl-prolyl cis/trans-isomerase activity, it accelerates folding in vitro, suggesting might be involved proteins. We describe a novel cyclophilin-like protein, S-cyclophilin, chick. Analysis S-cyclophilin cDNA revealed presence signal sequence followed by open reading frame coding for very similar cyclophilin,...
Alcohol abuse is highly prevalent, but little understood about the molecular causes. Here, we report that Ras suppressor 1 (Rsu1) affects ethanol consumption in flies and humans. Drosophila lacking Rsu1 show reduced sensitivity to ethanol-induced sedation. We required adult nervous system for normal it acts downstream of integrin cell adhesion molecule upstream Ras-related C3 botulinum toxin substrate (Rac1) GTPase regulate actin cytoskeleton. In an preference assay, global loss causes high...
Abstract To understand the molecular and neural mechanisms underlying alcohol addiction, many models ranging from vertebrates to invertebrates have been developed. In D rosophila melanogaster , behavioral paradigms assaying acute responses behaviors more closely modeling addiction emerged in recent years. However, both CAFÉ assay, similar a two‐bottle choice consumption as well conditioned odor preference, where ethanol is used reinforcer, are labor intensive low throughput. address this...
Jumonji (JmjC) domain proteins influence gene expression and chromatin organization by way of histone demethylation, which provides a means to regulate the activity genes across genome. JmjC have been associated with many human diseases including various cancers, developmental neurological disorders, however, shared biology possible common contribution organismal development tissue homeostasis all remains unclear. Here, we systematically tested function 13 Drosophila genes. Generation...
Abstract In genetic screens for Drosophila mutations affecting circadian locomotion rhythms, we have isolated six new alleles of the timeless (tim) gene. Two these cause short-period rhythms 21–22 hr in constant darkness, and four result long-period cycles 26–28 hr. All are semidominant. Studies interactions some tim with period-altering period (per) indicate that close to multiplicative; a given allele changes length background by fixed percentage, rather than number hours. The timL1 was...
Abstract Background Gene regulation is critical for proper cellular function. Next-generation sequencing technology has revealed the presence of regulatory networks that regulate gene expression and essential functions. Studies investigating epigenome have begun to uncover complex mechanisms regulating transcription. Assay transposase-accessible chromatin by (ATAC-seq) quickly becoming assay choice many epigenomic investigations. However, whether intervention-mediated changes in accessible...
Alcohol use disorders affect millions of individuals. However, the genes and signaling pathways involved in behavioral ethanol responses addiction are poorly understood. Here we identify a conserved biochemical pathway that underlies sedating effects Drosophila . Mutations Arf6 small GTPase cause hypersensitivity to ethanol-induced sedation. We show functions adult nervous system control behavior. also find Arfaptin protein directly binds activated forms Rac1 GTPases, mutants display...