A5021958189- Neurobiology and Insect Physiology Research
- Cellular transport and secretion
- Retinal Development and Disorders
- Autophagy in Disease and Therapy
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Lipid Membrane Structure and Behavior
- Axon Guidance and Neuronal Signaling
- Insect and Arachnid Ecology and Behavior
- ATP Synthase and ATPases Research
- Genetics, Aging, and Longevity in Model Organisms
- Developmental Biology and Gene Regulation
- CRISPR and Genetic Engineering
- Neuroscience, Education and Cognitive Function
- Physiological and biochemical adaptations
- Photosynthetic Processes and Mechanisms
- Neuroscience and Neural Engineering
- Endoplasmic Reticulum Stress and Disease
- Neural dynamics and brain function
- Alzheimer's disease research and treatments
- Plant and Biological Electrophysiology Studies
- Parkinson's Disease Mechanisms and Treatments
- Animal Behavior and Reproduction
- Cellular Mechanics and Interactions
- Insect Resistance and Genetics
Freie Universität Berlin
2016-2025
Charité - Universitätsmedizin Berlin
2015-2020
Center for Systems Biology
2009-2015
Southwestern Medical Center
2009-2015
The University of Texas Southwestern Medical Center
2007-2015
Czech Academy of Sciences, Institute of Physiology
2010
Baylor College of Medicine
2000-2008
Howard Hughes Medical Institute
2000-2008
Stanford University
2007
Children's Cancer Center
2003
Abstract The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each gene by the insertion of a single transposable element. As part this effort, transposons in >30,000 fly strains were localized and analyzed relative predicted structures. Approximately 6300 lines that maximize genomic coverage selected be sent Bloomington Stock Center for public distribution, bringing size BDGP disruption collection 7140 lines. It now includes individual 5362 13,666 currently annotated...
We present the first three-dimensional map of antennal lobe Drosophila melanogaster, based on confocal microscopic analysis glomeruli stained with neuropil-specific monoclonal antibody nc82. The stacks allowed us to identify according criteria shape, size, position, and intensity labeling. Forty were labeled by nc82, eight which have not been described before. Three previously shown exclusively backfills discernible in nc82 stainings. distinguish three classes glomeruli: (1) "landmark" that...
Rab proteins are small GTPases that play important roles in transport of vesicle cargo and recruitment, association motor other with vesicles, docking fusion vesicles at defined locations. In vertebrates, >75 genes have been identified, some which intensively studied for their endosome synaptic trafficking. Recent studies the functions certain revealed specific mediating developmental signal transduction. We begun a systematic genetic study 33 Drosophila. Most fly clearly related to...
During animal development, organ size is determined primarily by the amount of cell proliferation, which must be tightly regulated to ensure generation properly proportioned organs. However, little known about molecular pathways that direct cells stop proliferating when an has attained its proper size. We have identified mutations in a novel gene,shar-pei, required for termination proliferation during Drosophila imaginal disc development. Clones ofshar-pei mutant discs produce enlarged...
Presenilin (PSEN) deficiency is accompanied by accumulation of endosomes and autophagosomes, likely caused impaired endo-lysosomal fusion. Recently, Lee et al. (2010. Cell. doi: http://dx.doi.org/10.1016/j.cell.2010.05.008) attributed this phenomenon to PSEN1 enabling the transport mature V0a1 subunits vacuolar ATPase (V-ATPase) lysosomes. In their view, mediates N-glycosylation in endoplasmic reticulum (ER); consequently, PSEN prevents glycosylation, compromising delivery unglycosylated...
Wallerian degeneration refers to a loss of the distal part an axon after nerve injury. slow (Wld(s)) mice overexpress chimeric protein containing NAD synthase NMNAT (nicotinamide mononucleotide adenylyltransferase 1) and exhibit delay in axonal degeneration. Currently, conflicting evidence raises questions as whether is protecting factor its enzymatic activity required for such possible function. Importantly, link between nmnat at present solely based on overexpression studies enzymatically...
Diversity from development When given a line to follow, some fruit flies do so carefully and others weave. Linneweber et al. now show that these behaviors are stable for an individual but diverse in isogenic population. Key generating diversity the population is inherent chaos of normal development. A set neurons visual system wired up variable manner, resulting brain circuit asymmetry unique each fly guides its line-walking behavior. With more circuit, better able orient line. Science ,...
Acetylcholine is the major excitatory neurotransmitter in central nervous system of insects. Mutant analysis Dalpha7 nicotinic acetylcholine receptor (nAChR) Drosophila shows that it required for giant fiber-mediated escape behavior. The protein enriched dendrites fiber, and electrophysiological fiber circuit showed sensory input to disrupted, as transmission at an identified cholinergic synapse between peripherally synapsing interneuron dorsal lateral muscle motor neuron. Moreover, we found...
The vesicular adenosine triphosphatase (v-ATPase) is a proton pump that acidifies intracellular compartments. In addition, mutations in components of the membrane-bound v-ATPase V0 sector cause acidification-independent defects yeast, worm, fly, zebrafish, and mouse. this study, we present dual function for neuron-specific subunit a1 orthologue v100 Drosophila melanogaster. A mutant selectively disrupts translocation rescues previously characterized synaptic vesicle fusion defect with early...
Filopodial dynamics are thought to control growth cone guidance, but the types and roles of underlying neural circuit assembly in a living brain largely unknown. To address this issue, we have developed long-term, continuous, fast high-resolution imaging from axon synapse formation cultured Drosophila brains. Using R7 photoreceptor neurons as model show that >90% filopodia exhibit fast, stochastic persist despite ongoing stepwise layer formation. Correspondingly, cones stabilize early...
The small GTPase Rab7 is a key regulator of endosomal maturation in eukaryotic cells. Mutations rab7 are thought to cause the dominant neuropathy Charcot-Marie-Tooth 2B (CMT2B) by gain-of-function mechanism. Here we show that loss rab7, but not overexpression CMT2B mutants, causes adult-onset neurodegeneration Drosophila model. All mutant proteins retain 10–50% function based on quantitative imaging, electrophysiology, and rescue experiments sensory motor neurons vivo. Consequently,...
Neurons are highly polarized cells that require continuous turnover of membrane proteins at axon terminals to develop, function, and survive. Yet, it is still unclear whether protein degradation requires transport back the cell body or also occurs locally terminal, where live observation sorting has remained a challenge. Here, we report direct two cargo-specific mechanisms based on live-imaging approach in intact Drosophila brains. We show different acidification-sensing cargo probes sorted...