A5083586688- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Tissue Engineering and Regenerative Medicine
- Cellular transport and secretion
- Hippo pathway signaling and YAP/TAZ
- Hereditary Neurological Disorders
- Electrospun Nanofibers in Biomedical Applications
- Connective tissue disorders research
- Cancer Mechanisms and Therapy
- Lysosomal Storage Disorders Research
- Blood properties and coagulation
- Chromatin Remodeling and Cancer
- Cell Image Analysis Techniques
- Connective Tissue Growth Factor Research
- Retinal Development and Disorders
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Signaling Pathways in Disease
- Plasmonic and Surface Plasmon Research
- Lipid metabolism and biosynthesis
- CAR-T cell therapy research
- Neurological Disorders and Treatments
- RNA Interference and Gene Delivery
- Sphingolipid Metabolism and Signaling
- Ovarian cancer diagnosis and treatment
Johns Hopkins University
2018-2022
Johns Hopkins Medicine
2020
National Taiwan University
2013-2017
The small GTPase Rab7 is a key regulator of endosomal maturation in eukaryotic cells. Mutations rab7 are thought to cause the dominant neuropathy Charcot-Marie-Tooth 2B (CMT2B) by gain-of-function mechanism. Here we show that loss rab7, but not overexpression CMT2B mutants, causes adult-onset neurodegeneration Drosophila model. All mutant proteins retain 10–50% function based on quantitative imaging, electrophysiology, and rescue experiments sensory motor neurons vivo. Consequently,...
Introduction: A key obstacle in the creation of engineered cardiac tissues clinically relevant sizes is limited diffusion oxygen and nutrients. Thus, there a need for organized vascularization within three-dimensional (3D) tissue environment. Human induced pluripotent stem cell (hiPSC)-derived early vascular cells (EVCs) have shown to improve organization networks hydrogels. We hypothesize that introduction EVCs into 3D microtissue spheroids will lead increased microvascular formation...
Impaired clearance of amyloid-β peptide (Aβ) leads to abnormal extracellular accumulation this neurotoxic protein that drives neurodegeneration in sporadic Alzheimer's disease (AD). Connective tissue growth factor (CTGF/CCN2) expression is elevated plaque-surrounding astrocytes AD patients. However, the role CTGF pathogenesis remains unclear. Here we characterized neuroprotective activity CTGF. We found facilitated Aβ uptake and subsequent degradation within primary glia neuroblastoma cells....
The axial stiffness of DNA origami is determined as a function key nanostructural characteristics. Different constructs two-helix nanobeams with specified densities nicks and Holliday junctions are synthesized stretched by fluid flow. Implementing single particle tracking to extract force-displacement curves enables the measurement values at enthalpic elasticity regime, i.e. for forces larger than 15 pN. Comparisons between ligated nicked helices show that latter exhibit nearly two-fold...
Abstract Heart disease is one of the leading death causes in developed countries. To facilitate heart rehabilitation, engineered cardiac implantation has emerged as a promising alternative to organ transplantation. Currently there no quantitative standard ensure safety and functionality tissues intended for clinical uses. In anticipation application patients, suite methods assembled evaluate mechanical characteristics critical functions, including contractility, viscoelasticity, dynamic...
It has been demonstrated that geometry can affect cell behaviors. Though curvature-sensitive proteins at the nanoscale are studied, it is unclear how cells sense curvature cellular and multicellular levels. To characterize determine mechanisms of curvature-dependent behaviors, we grow on open channels 60-µm radius. We found cortical F-actin 1.2-fold more enriched in epithelial grown curved surface compared to flat control. observed myosin activity required promote formation. Furthermore,...