A5082880842- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Gold and Silver Nanoparticles Synthesis and Applications
- Carbon and Quantum Dots Applications
- Diamond and Carbon-based Materials Research
- Molecular Sensors and Ion Detection
- Advanced Fluorescence Microscopy Techniques
- Nonlinear Optical Materials Studies
- Electrochemical sensors and biosensors
- Nanoparticle-Based Drug Delivery
- Advanced Electron Microscopy Techniques and Applications
- Cancer, Lipids, and Metabolism
- Advanced Fiber Laser Technologies
- Near-Field Optical Microscopy
- Nanocluster Synthesis and Applications
- Graphene and Nanomaterials Applications
- Breast Lesions and Carcinomas
- Breast Cancer Treatment Studies
- Electric Power Systems and Control
- Extraction and Separation Processes
- Nanomaterials for catalytic reactions
- Carbon Nanotubes in Composites
- Mercury impact and mitigation studies
- Cell Image Analysis Techniques
Guizhou Minzu University
2024-2025
Johns Hopkins University
2019-2024
Shanghai Institute of Applied Physics
2011-2024
Wuhan University
2006-2024
Zhongnan Hospital of Wuhan University
2022-2024
Shandong Normal University
2021-2024
Chinese Academy of Sciences
2011-2024
Huaqiao University
2023
Sixth Affiliated Hospital of Sun Yat-sen University
2012
Sun Yat-sen University
2012
Designed oligonucleotides can self-assemble into DNA nanostructures with well-defined structures and uniform sizes, which provide unprecedented opportunities for biosensing, molecular imaging, drug delivery. In this work, we have developed functional, multivalent by appending unmethylated CpG motifs to three-dimensional tetrahedra. These small-sized functional are compact, mechanically stable, noncytotoxic. We demonstrated that resistant nuclease degradation remain substantially intact in...
Abstract DNA is typically impermeable to the plasma membrane due its polyanionic nature. Interestingly, several different nanostructures can be readily taken up by cells in absence of transfection agents, which suggests new opportunities for constructing intelligent cargo delivery systems from these biocompatible, nonviral nanocarriers. However, underlying mechanism entry into remains unknown. Herein, we investigated endocytotic internalization and subsequent transport tetrahedral (TDNs)...
Right out of the (logic) gate: Logic gates made from 3D DNA nanotetrahedra were constructed that are responsive to various ions, small molecules, and short strands DNA. By including dynamic sequences in one or more edges tetrahedra, a FRET signal can be generated manner AND, OR, XOR, INH logic gates, as well half-adder circuit (see scheme). These also applied intracellular detection ATP. Detailed facts importance specialist readers published "Supporting Information". Such documents...
Mechanistic understanding of the endocytosis and intracellular trafficking nanoparticles is essential for designing smart theranostic carriers. Physico-chemical properties, including size, clustering surface chemistry regulate their cellular uptake transport. Significantly, even single could cluster intracellularly, yet state subsequent are not well understood. Here, we used DNA-decorated gold (fPlas-gold) as a dually emissive fluorescent plasmonic probe to examine states Evidence from...
Delivery vehicles: Gold nanoparticles (AuNPs) have been used as a nanocarrier to non-invasively deliver synthetic cytosine–phosphate–guanosine (CpG) oligodeoxynucleotides (ODNs) into cells. Compared unconjugated single-stranded CpG ODNs, self-assembled polyvalent CpG-AuNP conjugates enhance the efficiency of cellular uptake and stimulate secretion cytokines (see picture). Detailed facts importance specialist readers are published ”Supporting Information”. Such documents peer-reviewed, but...
Graphene oxide (GO), has created an unprecedented opportunity for development and application in biology, due to its abundant functional groups well water solubility. Recently, the potential toxicity of GO environment humans garnered more attention. In this paper, we systematically studied cytotoxicity nanosheets via examining effect on morphology, viability differentiation a human neuroblastoma SH-SY5Y cell line, which was ideal model used study neuronal disease vitro. The results suggested...
Bio-logisch: Logikgatter aus dreidimensionalen DNA-Nanotetraedern reagieren auf verschiedene Ionen, niedermolekulare Verbindungen und kurze DNA-Stränge. Durch Einbau dynamischer Sequenzen in eine oder mehrere Tetraederkanten lässt sich ein FRET-Signal der Art von AND-, OR-, XOR-, INH- Halbaddier-Logikgattern generieren (siehe Schema). Diese DNA-Logikgatter wurden auch für die intrazelluläre Detektion ATP eingesetzt. Detailed facts of importance to specialist readers are published as...
Genomic oxidation and alkylation are two of the most important forms cytotoxic damage that may induce mutagenesis, carcinogenicity, teratogenicity. Human 8-oxoguanine (hOGG1) alkyladenine DNA glycosylases (hAAG) responsible for major oxidative alkylative repair, their aberrant activities cause repair deficiencies associated with a variety human diseases, including cancers. Due to complicated catalytic pathways hydrolysis mechanisms, simultaneous accurate detection multiple has remained great...
N6-Methyladenine (6mdA) and N4-methylcytosine (4mdC) are the two most dominant DNA modifications in both prokaryotes eukaryotes, but standard hybridization-based techniques cannot be applied for 6mdA/4mdC assay. Herein, we demonstrate silver-coordinated Watson–Crick pairing-driven three-dimensional (3D) walker locus-specific detection of genomic at single-molecule level. 6mdA-DNA 4mdC-DNA can selectively hybridize with binding probes (BP1 BP2) to form 6mdA-DNA-BP1 4mdC-DNA-BP2 duplexes. The...
Myocardial ischemia/reperfusion injury (MIRI) is the leading cause of irreversible myocardial damage. A pivotal pathogenic factor (I/R)-induced cardiomyocyte ferroptosis, marked by iron overload and lipid peroxidation. However, impact droplet (LD) changes on I/R-induced ferroptosis unclear. In this study, an aggregation-induced emission probe, TPABTBP developed that used for imaging dynamic in LD during ferroptosis. exhibits excellent LD-specificity, superior capability monitoring lipophagy,...
Abstract Plasmonic nanocavities are highly desirable for optical sensing because of their singular ability to confine light into deep subwavelength volumes. Yet, it remains profoundly challenging fabricate structurally resilient with high fidelity, and obtain direct, noninvasive visualization the plasmonic hotspots within such constructs. Herein, precise robust nanocavities, entitled DNA‐silicified template Raman beacon (DNA‐STROBE), engineered by using silicified DNA scaffolds spatial...
Nanodiamonds (NDs) possess many excellent physical and chemical properties that make them attractive materials for applications in biomedicine. In this paper, the adsorption delivery of a large amount sodium ions into cell interior by NDs serum-free medium is demonstrated. The excess inside cells induce osmotic stresses followed swelling an increase intracellular levels calcium reactive oxygen species (ROS), which leads to severe cellular damage. complete culture medium, however, serum...
Abstract DNA is typically impermeable to the plasma membrane due its polyanionic nature. Interestingly, several different nanostructures can be readily taken up by cells in absence of transfection agents, which suggests new opportunities for constructing intelligent cargo delivery systems from these biocompatible, nonviral nanocarriers. However, underlying mechanism entry into remains unknown. Herein, we investigated endocytotic internalization and subsequent transport tetrahedral (TDNs)...
The rapidly increasing need for systems biology stimulates the development of supermultiplex (SM) methods simultaneously labeling multiple biomolecules/cells with distinct colors. Here we report DNA-engineered fractal nanoplasmonic labels ultrahigh brightness and photostability SM imaging in single cells. These color-resolvable have a uniform size ∼50 nm an inner hollow gap ∼1 nm. outer shell morphology is highly tunable tip aspect ratio covering range δ = 0.29–1.66, which supports plasmonic...
Abstract Nitrogen‐vacancy (NV) centers in nanodiamond hold great promise for creating superior biological labels and quantum sensing methods. Yet, inefficient photon generation extraction from excited NV restrict the achievable sensitivity temporal resolution. Herein, an entirely complementary route featuring pyramidal hyperbolic metasurface is reported to modify spontaneous emission of centers. Fabricated using nanosphere lithography, consists alternatively stacked silica–silver thin films...
Objectives We aim to pharmacologically downregulate heat shock protein 27 (HSP27) through triptolide (TPL) improve the drug sensitivity of pancreatic cancer cisplatin (DDP). Methods In vitro, we assessed cell viability and apoptosis by combination TPL DDP in gemcitabine-resistant human carcinoma PANC-1 MIA PaCa-2 lines examined effect silencing HSP27 a small interfering RNA on cytotoxicity induced or DDP. vivo, apply with xenograft model test synergic action. Results Triptolide cooperates...
While nitrogen-vacancy (NV) centers in diamonds have emerged as promising solid-state quantum emitters for sensing applications, the tantalizing possibility of coupling them with photonic or broadband plasmonic nanostructures to create ultrasensitive biolabels has not been fully realized. Indeed, it remains technologically challenging free-standing hybrid diamond-based imaging nanoprobes enhanced brightness and high temporal resolution. Herein, we leverage bottom-up DNA self-assembly develop...
Transportvehikel: Gold-Nanopartikel (AuNPs) werden als Träger für die nichtinvasive Einschleusung von synthetischen Cytosinphosphatguanosin(CpG)-Oligodesoxynucleotiden (ODNs) in Zellen genutzt. Verglichen mit nichtkonjugierten einsträngigen CpG-ODNs erhöhen selbstorganisierte polyvalente CpG-AuNP-Konjugate Effizienz der Zelleinschleusung und stimulieren Sekretion Cytokinen (siehe Bild). Detailed facts of importance to specialist readers are published as "Supporting Information". Such...
Colloidal metamaterials are highly desired artificial materials that recapitulate the structure of simple molecules. They exhibit exceptional functionalities conferred by organization and specific interaction among constituent elements. Harvesting such exquisite attributes for potential applications necessitates establishing precise control over their structural configuration with high precision. Yet, creating molecule-like small clusters colloidal remains profoundly challenging, as a lack...