A5084817298- Calcium signaling and nucleotide metabolism
- Autophagy in Disease and Therapy
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Adenosine and Purinergic Signaling
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Cervical Cancer and HPV Research
- Ion Channels and Receptors
- Gut microbiota and health
- interferon and immune responses
- Signaling Pathways in Disease
- Phagocytosis and Immune Regulation
- Advanced biosensing and bioanalysis techniques
- Malaria Research and Control
- vaccines and immunoinformatics approaches
- Plant-derived Lignans Synthesis and Bioactivity
- Genital Health and Disease
- Vaccine Coverage and Hesitancy
- Magnolia and Illicium research
- Long-Term Effects of COVID-19
- Heavy Metal Exposure and Toxicity
- PARP inhibition in cancer therapy
- Sphingolipid Metabolism and Signaling
- Climate Change and Health Impacts
Shanghai Jiao Tong University
2014-2025
Shanghai First People's Hospital
2016-2025
Southwest Jiaotong University
2023-2025
Zhejiang University
2023-2025
First Affiliated Hospital Zhejiang University
2025
University of Pennsylvania
2025
The Seventh Affiliated Hospital of Sun Yat-sen University
2020-2024
Sun Yat-sen University
2020-2024
Shanghai University of Traditional Chinese Medicine
2024
Southwest University
2024
Abstract Recent reports have suggested that the gut microbiota is involved in progression of colorectal cancer (CRC). The composition CRC precursors has not been adequately described. To characterize structure adherent this disease, we conducted pyrosequencing-based analysis 16S rRNA genes to determine bacterial profile normal colons (healthy controls) and adenomas (CRC precursors). Adenoma mucosal biopsy samples adjacent colonic mucosa from 31 patients with 20 healthy volunteers were...
Autophagy is a catabolic lysosomal degradation process essential for cellular homeostasis and cell survival. Dysfunctional autophagy has been associated with wide range of human diseases, e.g., cancer neurodegenerative diseases. A large number small molecules that modulate have widely used to dissect this some them, chloroquine (CQ), might be ultimately applied treat variety autophagy-associated Here we found vacuolin-1 potently reversibly inhibited the fusion between autophagosomes...
Background: Information regarding viral shedding in children with coronavirus disease 2019 (COVID-19) was limited. This study aims to investigate the clinical and laboratory characteristics associated mild COVID-19. Methods: The information of 110 COVID-19 at Wuhan Children’s Hospital, Wuhan, China, from January 30 March 10, 2020, were analyzed retrospectively. Results: median age 6 years old. period 15 days (interquartile range [IQR], 11–20 days) as measured illness onset discharge. shorter...
Exposure to some conventional trace elements has been found be associated with miscarriage; however, evidence for combined exposure is inconclusive. Therefore, it important explore the joint associations between toxic and essential miscarriage.This cross-sectional study measured a wide range of element levels in whole blood pregnant women by using inductively coupled plasma mass spectrometry. The individual miscarriage were appraised logistic regression model. Multi-exposure models,...
Abstract Background Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin, isolated from the traditional Chinese herb Artemisia annua, is recommended as first-line anti-malarial drug with low toxicity. DHA has been shown to possess promising anticancer activities and induce cancer cell death through apoptotic pathways, although molecular mechanisms are not well understood. Methods In this study, counting kit (CCK-8) assay was employed evaluate survival DHA-treated ASTC-a-1...
Reactive oxygen species (ROS) have been commonly accepted as inducers of autophagy, and autophagy in turn is activated to relieve oxidative stress. Yet, whether how stress, generated various human pathologies, regulates remains unknown. Here, we mechanistically studied the role TRPM2 (transient receptor potential cation channel subfamily M member 2)-mediated Ca2+ influx stress-mediated regulation. On one hand, demonstrated that stress triggered TRPM2-dependent inhibit induction early which...
YKL-40 is also called chitinase-3-like-1 (CHI3L1) protein and may be a marker for asthma. The aims of the present study were to investigate whether serum levels are stable or decreased in patients with asthma after appropriate treatment evaluate correlation lung function control test (ACT) results. A total 103 asthmatic (mean age 33.1 ± 0.9 years) diagnosed enrolled our study. All underwent detailed clinical examination completed ACT questionnaire, measurement, spirometry before (visit 1) 8...
Abstract The long-term immunity and functional recovery after SARS-CoV-2 infection have implications in preventive measures patient quality of life. Here we analyzed a prospective cohort 121 recovered COVID-19 patients from Xiangyang, China at 1-year diagnosis. Among them, chemiluminescence immunoassay-based screening showed 99% (95% CI, 98–100%) seroprevalence 10–12 months infection, comparing to 0.8% 0.7–0.9%) the general population. Total anti-receptor-binding domain (RBD) antibodies...
Many anticancer agents exert cytotoxicity and trigger apoptosis through the induction of mitochondrial dysfunction. Mitophagy, as a key quality control mechanism, can remove damaged mitochondria in an effective timely manner, which may result drug resistance. Although implication mitophagy neurodegenerative diseases has been extensively studied, role mechanism tumorigenesis cancer therapy are largely unknown. In recent study, we found that inhibition PINK1-PRKN-mediated significantly enhance...
N6-Methyladenine (6mdA) and N4-methylcytosine (4mdC) are the two most dominant DNA modifications in both prokaryotes eukaryotes, but standard hybridization-based techniques cannot be applied for 6mdA/4mdC assay. Herein, we demonstrate silver-coordinated Watson–Crick pairing-driven three-dimensional (3D) walker locus-specific detection of genomic at single-molecule level. 6mdA-DNA 4mdC-DNA can selectively hybridize with binding probes (BP1 BP2) to form 6mdA-DNA-BP1 4mdC-DNA-BP2 duplexes. The...
Abstract Interleukin 31 (IL-31) is a novel T helper type 2 effector cytokine that plays an important role in the pathogenesis of allergic diseases. However, its human asthma remains unclear. The aim this study was to measure IL-31 levels serum, bronchoalveolar lavage fluid (BALF) and bronchial tissue asthmatics healthy subjects identify possible correlation disease severity. We quantified serum patients with (n = 44), as well controls 22). Of these subjects, 9 five underwent bronchoscopy...
Mitochondria as a signaling platform play crucial roles in deciding cell fate. Many classic anticancer agents are known to trigger death through induction of mitochondrial damage. Mitophagy, one selective autophagy, is the key quality control that effectively removes damaged mitochondria. However, precise mitophagy tumorigenesis and agent treatment remain largely unclear. Here, we examined functional implication properties magnolol, natural product isolated from herbal Magnolia officinalis....
The gut is involved in the development of acute pancreatitis (AP). Increased focus being given to role microbiota pathogenesis AP. Nevertheless, there currently no available evidence regarding composition fungal microorganisms intestines patients with In this study, we sequenced ITS rRNA gene amplicons and examined intestinal feces from 11 AP (the test group) 15 healthy people control group). Additionally, relationship between fungus clinical biochemical markers. Results showed a decline...
In modern clinical settings, interdisciplinary reasoning skills and associated education are pivotal should be encouraged for residency training. An course on was developed residents based ADDIE (Analysis, Design, Development, Implementation, Evaluation) model. We collected frequently encountered consultation cases as our teaching resources with the methods of scenario case-based learning. Finally, learning (CCBL) method established. At end course, summative evaluations were carried out to...
Importance Guidelines recommend that intensive care unit (ICU) clinicians consider prognosis and offer a comfort-focused treatment alternative to patients with limited prognoses promote preference-sensitive decisions. Objective To determine whether nudging ICU adhere communication guidelines improves outcomes among critically ill at high risk of death or severe functional impairment. Design, Setting, Participants This 4-arm pragmatic, stepped-wedge, cluster randomized trial (conducted...
Mitochondria serve as the primary source of intracellular reactive oxygen species (ROS), which play a critical role in orchestrating cell death pathways such pyroptosis various types cancers. PINK1-mediated mitophagy effectively removes damaged mitochondria and reduces detrimental ROS levels, thereby promoting survival. However, regulation by PINK1 neuroblastoma remains unclear. In this study, we demonstrate that inhibition or deficiency sensitizes signaling promotes cells via...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is an endogenous Ca2+ mobilizing nucleotide presented in various species. NAADP mobilizes from acidic organelles through two pore channel 2 (TPC2) many cell types and it has been previously shown that can potently induce neuronal differentiation PC12 cells. Here we examined the role of TPC2 signaling neural mouse embryonic stem (ES) We found expression was markedly decreased during initial ES entry into progenitors, levels gradually...
Intracellular pH (pHi) and Ca(2+) regulate essentially all aspects of cellular activities. Their inter-relationship has not been mechanistically explored. In this study, we used bases acetic acid to manipulate the pHi. We found that transient pHi rise induced by both organic inorganic bases, but acidification acid, produced elevation cytosolic Ca(2+). The sources increase are from endoplasmic reticulum (ER) pools as well influx. store-mobilization component was sensitive antagonists for...