A5085554978- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- Electrocatalysts for Energy Conversion
- Electrochemical Analysis and Applications
- Advanced battery technologies research
- Gold and Silver Nanoparticles Synthesis and Applications
- DNA and Nucleic Acid Chemistry
- Molecular Sensors and Ion Detection
- Electrochemical sensors and biosensors
- Conducting polymers and applications
- Molecular Junctions and Nanostructures
- Innovative Microfluidic and Catalytic Techniques Innovation
- Spectroscopy Techniques in Biomedical and Chemical Research
- Fuel Cells and Related Materials
- Nanoplatforms for cancer theranostics
- Immune Cell Function and Interaction
- Nanopore and Nanochannel Transport Studies
- Supramolecular Self-Assembly in Materials
- Nanoparticle-Based Drug Delivery
- MicroRNA in disease regulation
- Immunotherapy and Immune Responses
- Bacteriophages and microbial interactions
- Modular Robots and Swarm Intelligence
- Advanced Biosensing Techniques and Applications
East China Normal University
2017-2025
Zhejiang University
2020-2025
Fourth Affiliated Hospital of Anhui Medical University
2022
Anhui Medical University
2022
University of Shanghai for Science and Technology
2013-2018
Zhongshan Hospital
2018
Fudan University
2018
Abstract Conducting hydrogels provide great potential for creating designer shape‐morphing architectures biomedical applications owing to their unique solid–liquid interface and ease of processability. Here, a novel nanofibrous hydrogel with significant enzyme‐like activity that can be used as “ink” print flexible electrochemical devices is developed. The self‐assembled from guanosine (G) KB(OH) 4 simultaneous incorporation hemin into the G‐quartet scaffold, giving rise activity. rapid...
DNA-functionalized layered two-dimensional transition-metal dichalcogenides have attracted tremendous interest for constructing biosensors in recent years. In this work, we report diblock molecular beacons with poly-cytosine (polyC) tails anchored on molybdenum disulfide (MoS2) nanosheets as probes microRNA detection. The polyC block is adsorbed MoS2 and the beacon available hybridization to target; duplex-specific nuclease provides signal amplification by target recycling. By changing...
Rapid, sensitive, and reliable pathogen detection is growing in importance human health safety. In this work, we report a stochastic DNA dual-walker-based colorimetric biosensor for bacterial detection. the presence of target bacteria, two kinds released multiple walking strands are allowed continuous on Au nanoparticle (AuNP)-based 3D track, resulting destabilized aggregation AuNP-based probes. The induced color change from red to blue can serve as an analytical signal bacteria. We...
Despite smart drug delivery systems (DDSs) with high efficiency and improved efficacy for chemotherapy, precise release in targeted tumor cells a controllable way is still challenging. In this work, we develop DNA toehold switch-engineered spherical nucleic acid-templated hydrogel (SNAgel) on-site active burst of chemotherapeutic (tumor-killing) drugs target cancer cells. By designing ligand-specific sequences on hybridization chain reaction (HCR)-generated SNAgel, realize payloads wide...
The manipulation of cell–cell interactions promotes the study multicellular behavior, but it remains a great challenge for programming assembly in complex reaction pathways with multiple cell types. Here we report DNA circuit-based approach to cell–surface engineering programmable regulation interactions. circuits are designed on basis stem-loop-integrated hairpin motif, which has capability diverse molecular self-assembly and disassembly by sequential allosteric activation. Modifying...
Nanosized artificial antigen-presenting cells (aAPCs) with efficient signal presentation hold great promise for in vivo adoptive cell therapy. Here, we used DNA origami nanostructures as two-dimensional scaffolds to regulate the spatial of activating ligands at nanoscale construct high-effective aAPCs. The origami–based aAPC comprises costimulatory anti-CD28 antibody anchored three vertices and T receptor (TCR) peptide–major histocompatibility complex (pMHC) edges varying density. scaffold...
Abstract Molecular recognition at the biointerface plays a critical role in sensing molecular interactions (e.g., DNA hybridization) and extracellular changes, which can directly affect detection performance of biosensors sensitivity, specificity, response dynamics). However, conventional biointerfaces show low efficiency due to limited target accessibility. Engineering regulate orientation, spacing, density surface‐confined probes offer an effective approach improve interfaces. Over last...
The development of highly sensitive and selective methods for the detection microRNA (miRNA) has attracted tremendous attention because its importance in fundamental biological studies diagnostic applications. In this work, we develop DNA-encoded Raman-active anisotropic nanoparticles modified origami paper analytical devices (oPADs) rapid, sensitive, specific miRNA detection. were prepared using 10-mer oligo-A, -T, -C, -G to mediate growth Ag cubic seeds into (AgNPs) with different...
MicroRNAs (miRNAs) play significant regulatory roles in physiologic and pathologic processes are considered as important biomarkers for disease diagnostics therapeutics. Simple, fast, sensitive, selective detection of miRNAs, however, is challenged by their short length, low abundance, susceptibility to degradation, homogenous sequence. Here, we report a novel design nanoprobes highly sensitive miRNAs based on MoS2-loaded molecular beacons (MBs) duplex-specific nuclease (DSN)-mediated signal...
Highly sensitive and selective detection of heavy metal ions, such as Hg2+, is great importance because the contamination ions has been a serious threat to human health. Herein, we have developed poly-cytosine (polyC)-mediated surface-enhanced Raman scattering (SERS) nanotags sensor system for rapid, selective, Hg2+ based on thymidine-Hg2+-thymidine (T-Hg2+-T) coordination polyC-mediated activity. The SERS exploit mismatched T-T base pairs capture form T-Hg2+-T bridges, which induce...
Precise control of DNA-circuit functions facilitates the construction complex DNA networks to perform sophisticated functions. Inspired by optochemical genetics with high precision for controlling and studying neural photoregulation membrane receptors, we herein report optochemically controlled switching circuits regulating computation The DNA-switching involve a CG-C+ triplex-based switch that undergoes structural transition from triplex duplex states after exposure UV irradiation due...
Nucleic acid-based machines have sparked tremendous attention because of their potential applications in biosensing, drug delivery, and biocomputing. Herein, we construct an enzyme-propelled stochastic RNA walker that autonomously walks on a single wall carbon nanotube (SWCNT)-based one-dimensional (1D) track for miRNA imaging living cells. Driven by duplex-specific nuclease (DSN) with the capability selective digestion DNA DNA/RNA heteroduplexes, enables autonomous progressive walk SWCNTs,...
Peptide-MHC (pMHC) multimers have excelled in the detection of antigen-specific T cells and allowed phenotypic analysis using other reagents, but their use for low-affinity remains a challenge. Here we develop multimeric cell identifying reagent platform two-dimensional DNA origami scaffolds to spatially organize pMHCs (termed as dorimers) with nanoscale control. We show that these dorimers enhance binding avidity receptors (TCRs). The are able detect more mouse CD8
By incorporating hemin into G-quadruplex (G4) during cation-templated self-assembly between guanosine and KB(OH)4, we have constructed an artificial enzyme hydrogel (AEH)-based system for the highly sensitive selective detection of Pb2+. The sensing strategy is based on a Pb2+-induced decrease in AEH activity. Because higher efficiency Pb2+ stabilizing G4 compared with K+, ions substitute K+ trigger release from G4, thus giving rise to conformational interconversion accompanied by loss...
Abstract Developing smart material systems for performing different tasks in diverse environments remains challenging. Here, we show that by integrating stimuli‐responsive soft materials with multi‐mode reconfigurable DNA‐based chemical reaction circuits (D‐CRCs), it can control size change of microgels multiple pathways and adapt expansion behaviors to meet environments. We first use pH‐responsive intramolecular conformational switches regulating DNA strand displacement reactions (SDRs)....
The rapidly increasing need for systems biology stimulates the development of supermultiplex (SM) methods simultaneously labeling multiple biomolecules/cells with distinct colors. Here we report DNA-engineered fractal nanoplasmonic labels ultrahigh brightness and photostability SM imaging in single cells. These color-resolvable have a uniform size ∼50 nm an inner hollow gap ∼1 nm. outer shell morphology is highly tunable tip aspect ratio covering range δ = 0.29–1.66, which supports plasmonic...