A5044877088- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Nanoplatforms for cancer theranostics
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Biosensors and Analytical Detection
- Gold and Silver Nanoparticles Synthesis and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- CAR-T cell therapy research
East China Normal University
2019-2025
Nanosized artificial antigen-presenting cells (aAPCs) with efficient signal presentation hold great promise for in vivo adoptive cell therapy. Here, we used DNA origami nanostructures as two-dimensional scaffolds to regulate the spatial of activating ligands at nanoscale construct high-effective aAPCs. The origami–based aAPC comprises costimulatory anti-CD28 antibody anchored three vertices and T receptor (TCR) peptide–major histocompatibility complex (pMHC) edges varying density. scaffold...
Peptide-MHC (pMHC) multimers have excelled in the detection of antigen-specific T cells and allowed phenotypic analysis using other reagents, but their use for low-affinity remains a challenge. Here we develop multimeric cell identifying reagent platform two-dimensional DNA origami scaffolds to spatially organize pMHCs (termed as dorimers) with nanoscale control. We show that these dorimers enhance binding avidity receptors (TCRs). The are able detect more mouse CD8
Current in vitro models struggle to balance the complexity of human diseases with suitability for large-scale drug tests. While 3D cultures simulate tissues, they lack cellular intricacy, and integrating these high-throughput screening remains a challenge. Here, we introduce method that uses self-assembling nucleic acid nanostructures decorated living cells, termed NACs, create spheroids customizable layout. To demonstrate its uniqueness, our effectively creates designer by combining...
Visualizing the spatial distribution of antigen-specific T cells is essential for understanding immune responses and improving therapeutic strategies. However, detecting low-affinity enhancing signals from low-abundance populations remain challenging due to limitations in sensitivity. Here, we report DNA origami scaffold-based peptide-major histocompatibility complex multimers (DOS-pMHCs) with precise organization pMHC signaling molecules on nanoscale enhanced situ visualization cells. The...
Abstract In response to specific stimuli, dynamic covalent materials enable the generation of new structures by reversibly forming/breaking chemical bonds, thus showing great potential for application in controlled drug release. However, using chemistry program drug‐delivery kinetics remains challenging. Herein, an situ polymerization‐generated DNA‐scaffolded disulfide redox network (DdiSRN) is reported which nucleic acids are used as a scaffold bonds. The constructed DdiSRN allows selective...
Abstract In vitro models are crucial for drug screening, yet they often fail to accurately reflect human physiological responses. While 3D cell cultures aim simulate tissues, many lack the detailed complexity and interaction of various types found in actual tissues. Additionally, integrating these with high-throughput screening remains a challenge. Current can't strike balance between capturing diseases being suitable large-scale tests. Here we introduce method that uses self-assembling...