A5103123000- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Organ Transplantation Techniques and Outcomes
- Renal Transplantation Outcomes and Treatments
- MicroRNA in disease regulation
- Pharmacological Effects and Toxicity Studies
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Circular RNAs in diseases
- 3D Printing in Biomedical Research
- Drug Transport and Resistance Mechanisms
- Liver Disease and Transplantation
- Drug-Induced Hepatotoxicity and Protection
- Viral-associated cancers and disorders
- Tissue Engineering and Regenerative Medicine
- Metabolism and Genetic Disorders
- Innovative Microfluidic and Catalytic Techniques Innovation
- Pancreatitis Pathology and Treatment
- GDF15 and Related Biomarkers
- Innovation and Socioeconomic Development
- Immune cells in cancer
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advancements in Transdermal Drug Delivery
- CRISPR and Genetic Engineering
- Viral gastroenteritis research and epidemiology
Renji Hospital
2020-2025
Shanghai Jiao Tong University
2014-2025
Shanghai Institute of Hematology
2024
Medizinische Hochschule Hannover
2018-2022
•Restoration of HNF4A via mRNA delivery improves functions fibrotic primary hepatocytes from both mice and humans.•The encapsulated in lipid nanoparticles can be delivered into the liver.•Lipid nanoparticle-mediated ameliorates fibrosis cirrhosis different chronic liver injury models.•Paraoxonase 1, a therapeutic target HNF4A, contributes to anti-fibrotic effects HNF4A.•HNF4A affects macrophage infiltration polarization as well hepatic stellate cell activation. Background & AimsTherapeutic...
Objective Liver fibrosis and cirrhosis resulting from chronic liver injury represent a major healthcare burden worldwide. Growth differentiation factor (GDF) 11 has been recently investigated for its role in rejuvenation of ageing organs, but diseases remained unknown. Here, we the expression function GDF11 fibrosis, common feature most diseases. Design We analysed patients with mouse model hepatic stellate cells (HSCs) as well other cell types. The functional relevance toxin-induced...
Abstract Partial hepatectomy is an essential surgical technique used to treat advanced liver diseases such as tumors, well for performing transplants from living donors. However, postoperative complications bleeding, abdominal adhesions, wound infections, and inadequate regeneration pose significant challenges increase morbidity mortality rates. A self‐repairing mixed hydrogel (O 5 H 2 /Cu 2+ /SCCK), containing stem cell derived cytokine (SCCK) human umbilical cord mesenchymal cells (HUMSCs)...
Current in vitro models struggle to balance the complexity of human diseases with suitability for large-scale drug tests. While 3D cultures simulate tissues, they lack cellular intricacy, and integrating these high-throughput screening remains a challenge. Here, we introduce method that uses self-assembling nucleic acid nanostructures decorated living cells, termed NACs, create spheroids customizable layout. To demonstrate its uniqueness, our effectively creates designer by combining...
Lipid nanoparticles (LNPs) have revolutionized nucleic acid delivery, enabling significant advances in mRNA-based therapeutics. While extensive research has focused on lipid composition, the impact of preparation solutions LNP performance remains underexplored. This study systematically investigated effects pH, salt type, and concentration across key solutions—mRNA aqueous, dilution, exchange, storage solutions—on physicochemical properties, stability, expression efficiency SM102-based...
Adeno-associated virus (AAV)-based vectors are considered efficient and safe gene delivery systems in therapy. We combined two guide RNA genes, Cas9, a self-linearizing repair template one vector (AIO-SL) to correct fumarylacetoacetate hydrolase (FAH) deficiency mice. The genome of 5.73 kb was packaged into VP2-depleted AAV particles (AAV2/8ΔVP2), which, however, did not improve cargo capacity. Reprogrammed hepatocytes were treated with AIO-SL.AAV2ΔVP2 subsequently transplanted, resulting...
Tacrolimus (TAC) is the backbone of an immunosuppressive drug used in most solid organ transplant recipients. A single nucleotide polymorphism (SNP) at position 6986G>A CYP3A5 has been notably involved pharmacokinetic variability TAC. It hypothesized that genotyping patients may provide a guideline for TAC therapeutic regimen. To further evaluate impact variants donors and recipients, ABCB1 ACE SNPs recipients on disposition, clinical laboratory data were retrospectively reviewed from 90...
Background & AimsSustained c-Jun N-terminal kinase (JNK) activation plays a major role in drug-induced liver injury (DILI). Stress-responsive microRNA-31 (miR-31) has been implicated regulating different cellular damage, and JNK could induce miR-31 expression. However, the regulatory of DILI not studied previously. We aimed to investigate whether ameliorate ascertain potential molecular mechanism.MethodsmiR-31 gene knockout (31-KO) wild-type C57BL/6J mice were used construct an acetaminophen...
The ability of the liver to regenerate and restore mass limits increasing mortality rate due life-threatening diseases. Successful regeneration is accomplished in multiple stages, which priming proliferation phases are well studied. However, regulatory pathways, specifically microRNA (miRNA)-mediated posttranscriptional regulation, prevent uncontrolled mediate termination regeneration, not understood. We identified differentially regulated miRNAs during phase after 2/3 partial hepatectomy...
The intracellular delivery of messenger (m)RNA holds great potential for the discovery and development vaccines therapeutics. Yet, in many applications, a major obstacle to clinical translation mRNA therapy is lack efficient strategy precisely deliver RNA sequence liver tissues cells. In this study, we synthesized virus-like mesoporous silica (V-SiO2) nanoparticles effectively therapeutic RNA. Then, cationic polymer polyethylenimine (PEI) was included further surface modification (V-SiO2-P)....
As a cytokine of the interleukin-6 family, cardiotrophin-1 (CT-1) has been shown to be an important endogenous protector in liver injury. Our study aimed investigate role CT-1 fibrosis pediatric cholestatic disease (PCLD). mRNA and protein expression levels were upregulated PCLD biopsy tissues compared with controls. Immunohistochemistry confocal microscopy sections showed that was predominantly expressed by biliary epithelium cells. Serum elevated significantly children exhibited moderate...
Abstract: Liver, the largest internal organ in vertebrates, plays an essential role metabolic processes body. Many factors, such as viruses and alcohol, can lead to function disorders or even liver failure. Every year more than a million patients suffer from various diseases. miRNAs have evolved important regulators development, homeostasis, dysfunction, regeneration. Increasing evidences shown that play diseases, including acute failure (ALF), chronic well carcinoma. In this review, we...
Liver fibrosis and cirrhosis resulting from long-standing liver damage represent a major health care burden worldwide. Members of the transforming growth factor β (TGF-β) family have been shown to play pivotal role in fibrogenesis as well multiple other organs. Growth differentiation (GDF) 11, member TGF-β family, has recently investigated for its rejuvenation aging However, function GDF11 remained elusive. Here, we expression chronic disease. We show that is upregulated patients with...
Objectives . Tacrolimus is characterized by high pharmacokinetic variability in combination with a narrow therapeutic range. However, influence of donor CYP3A5 genotype and graft‐to‐recipient body weight ratio (GRWR) on tacrolimus’ pharmacokinetics after pediatric living liver transplantation (LDLT) remains unclear. Methods A total 174 LDLT recipients (<6 y) were grouped according to genotypes (nonexpressor (NEX) or expressor (EX)) GRWR (<3.0% (SS, small‐size) ≥3.0% (LS, large‐size)):...
Objective Little information is available regarding the impact of cytochrome P450 (CYP) 3A5 on metabolism tacrolimus (TAC) in infant liver transplantation. Therefore, CYP3A5 genotype Chinese pediatric recipients (intestine) as well donors (graft liver) was analysed for purpose establishing an optimal dosage regimen children. Method Eighty-five patients treated with TAC were divided into following groups according to [expression *1 allele: expression (EX) and non-expression (NEX)] each...
Abstract In vitro models are crucial for drug screening, yet they often fail to accurately reflect human physiological responses. While 3D cell cultures aim simulate tissues, many lack the detailed complexity and interaction of various types found in actual tissues. Additionally, integrating these with high-throughput screening remains a challenge. Current can't strike balance between capturing diseases being suitable large-scale tests. Here we introduce method that uses self-assembling...
Messenger RNA (mRNA)-based drug technologies are expeditiously reaching the clinic and bear capacity to benefit millions of individuals globally. However, therapeutic targeting injuries that demand transient restoration proteins by mRNA delivery requires more in-depth analyses. In this study, we investigated applicability in liver fibrosis cirrhosis, which causes fatalities, annually. Here, envisioned demonstrate utility human transcription factor, hepatocyte nuclear factor alpha (HNF4A)...
Abstract Objectives: Tacrolimus is characterized by high pharmacokinetic variability in combination with a narrow therapeutic range. However, influence of donor CYP3A5 genotype and graft-to-recipient body weight ratio (GRWR) on tacrolimus’ pharmacokinetics after pediatric living liver transplantation (LDLT) remains unclear. Methods: A total 174 LDLT recipients (<6y) were grouped according to genotypes [non-expressor (NEX) or expressor (EX)] GRWR [<3.0% (SFS, small-for-size) ≥3.0% (LFS,...