A5103218335- Advanced Proteomics Techniques and Applications
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Glycosylation and Glycoproteins Research
- Metabolomics and Mass Spectrometry Studies
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Retinal Diseases and Treatments
- Mass Spectrometry Techniques and Applications
- Molecular Biology Techniques and Applications
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- Heat shock proteins research
- Glaucoma and retinal disorders
- RNA modifications and cancer
- Pregnancy and preeclampsia studies
- Retinal Imaging and Analysis
- Peptidase Inhibition and Analysis
- 14-3-3 protein interactions
- Physiological and biochemical adaptations
- Down syndrome and intellectual disability research
- Plant Gene Expression Analysis
- Bacterial biofilms and quorum sensing
- Toxin Mechanisms and Immunotoxins
- Proteoglycans and glycosaminoglycans research
Fudan University
2013-2025
Shanghai Stomatological Hospital
2019-2025
Shanghai Medical College of Fudan University
2011
Parkinson's disease (PD) is a common which occurs in aged people with chronic, progressive degenerative character of the central nervous system. Until now there no effective treatment method PD patients before they show obvious symptoms for prevention and early diagnosis. In order to find out specific biomarkers, two-dimensional liquid chromatography-tandem mass spectrometry coupled isobaric tags relative absolute quantification (iTRAQ) labeling was employed quantitatively identify...
An integrated multiple reaction monitoring (MRM)-based LC-MS/MS method to accurately differentiate and quantify K27 K36 modifications on H3.1 H3.3 in Arabidopsis thaliana .
Abstract Background Endometrial carcinoma is one of the most common gynecological malignancies in women. The diagnosis disease at early or premalignant stages crucial for patient's prognosis. To date, and follow-up endometrial hyperplasia require invasive procedures. Therefore, there considerable demand identification biomarkers to allow non-invasive detection these conditions. Methods In this study, we performed a quantitative proteomics analysis on serum samples from simple hyperplasia,...
Proteomics may help us better understand the changes of multiple proteins involved in oncogenesis and progression prostate cancer(PCa) identify more diagnostic prognostic biomarkers. The aim this study was to screen biomarkers PCa by proteomics analysis using isobaric tags for relative absolute quantification(iTRAQ).The patients undergoing biopsies were classified into 3 groups according pathological results: benign hyperplasia (BPH, n = 20), PCa(n 20) BPH with local prostatic...
Purpose: To evaluate vitreous humor (VH) protein expression profiles in patients with proliferative diabetic retinopathy (PDR), and without intravitreal injection (IVI) of anti-vascular endothelial growth factor (anti-VEGF) before vitrectomy. Methods: We enrolled consecutive PDR who needed pars plana vitrectomy (PPV) or IVI pan-retinal photocoagulation (PRP). Visual acuity, duration, treatment diabetes mellitus, ocular history, fundus examinations were recorded. VH samples collected...
Arginine methylation plays vital roles in the cellular functions of protozoan Trypanosoma brucei. The T. brucei arginine methyltransferase 6 (TbPRMT6) is a type I homologous to human PRMT6. In this study, we report crystal structures apo-TbPRMT6 and its complex with reaction product S-adenosyl-homocysteine (SAH). structure displays several features that are different from those PRMTs were structurally characterized previously, including four stretches insertion, absence strand β15, distinct...
Atherosclerotic cardiovascular disease is a common and severe complication of diabetes. There large need to identify the effective safety strategies on diabetic (DCVD). 9-PAHSA novel endogenous fatty acid, has been reported reduce blood glucose levels attenuate inflammation. We aim evaluate effects DCVD investigate possible mechanisms underlying it. Firstly, serum in human were detected by HPLC-MS/MS analysis. Then was synthesized purified. The gavaged db/db mice with 50 mg/kg for 4 weeks....
The coexisting post-translational modifications (PTMs) on histone H3 N-terminal tails were known to crosstalk between each other, indicating their interdependency in the epigenetic regulation pathways. H3K36 methylation, an important activating mark, was recently reported antagonize with PRC2-mediated H3K27 methylation possible mechanism during transcription process. On basis of our previous studies, we further integrated RP/HILIC liquid chromatography MRM mass spectrometry quantify PTMs...
ABSTRACT Objective This study aimed to identify candidate protein biomarkers from maternal serum for Down syndrome (DS) by integrated proteomic and bioinformatics analysis. Methods A pregnancy DS group of 18 women a control with the same number were prepared, proteins analyzed isobaric tags relative absolute quantitation mass spectrometry, differentially expressed (DS‐DEMSPs). Comprehensive analysis was then employed analyze DS‐DEMSPs both in this paper seven related publications. Results...
Abstract Background Methyl-CpG binding domain protein 1 (MBD1), a suppressor of gene transcription, may be involved in inactivation tumor genes during tumorigenesis. Over-expression MBD1 has been reported human pancreatic carcinomas. Methods In this study, we established MBD1-knock-down cancer cell line (BxPC-3) using stable RNA interference, to compare the proteomic changes between control and cells two-dimensional gel electrophoresis mass spectrometry. Results We identified five proteins...
This study aimed to investigate the protein expression profile of vitreous humor (VH) from pathologic myopic retinoschisis (PMRS) patients with or without intravitreal antivascular endothelial growth factor (anti-VEGF) therapy. VH samples PMRS were subjected proteomic analysis. Clinical data, including visual acuity, refractive error, and axial length, recorded, fundus optical coherence tomography was performed. Seven enrolled: 3 as control group, coexisting choroidal neovascularization...
Staphylococcus epidermidis is one of the major causative agents for nosocomial infections. To reveal pathogenesis factors, we performed comparative proteomic analysis invasive ATCC35984 and commensal ATCC12228 strains by two-dimensional gel electrophoresis matrix-assisted laser desorption/ionization-time flight-mass spectrometry. The differentially expressed proteins were involved in carbohydrate metabolism, sugar binding, lipid degradation amino acid binding. In addition, demonstrated that...
Objective To identify differentially expressed proteins from serum of pregnant women carrying a conotruncal heart defects (CTD) fetus, using proteomic analysis. Methods The study was conducted nested case-control design. 5473 maternal samples were collected at 14–18 weeks gestation. 9 CTD 10 with another CHD (ACHD) and 11 normal fetus selected the above samples, analyzed by isobaric tags for relative absolute quantitation (iTRAQ) coupled two-dimensional liquid chromatography-tandem mass...
The human hepatoma 3B cell line was chosen as an experimental model for in vitro test of drug screening. drugs included chlorophyllin and its derivatives such fluo-chlorophyllin, sodium copper chlorophyllin, iron chlorophyllin. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) method used this study to obtain the primary screening results. results showed that had best LC50 value. Proteomic analysis then performed further investigation effect addition Hep line. proteins...