A5042244659- Genomics, phytochemicals, and oxidative stress
- Acute Lymphoblastic Leukemia research
- Cancer, Hypoxia, and Metabolism
- Natural product bioactivities and synthesis
- Acute Myeloid Leukemia Research
- Beetle Biology and Toxicology Studies
- Retinoids in leukemia and cellular processes
- RNA modifications and cancer
- Antimicrobial Peptides and Activities
- Curcumin's Biomedical Applications
- Fungal Biology and Applications
- Peroxisome Proliferator-Activated Receptors
- Cancer-related Molecular Pathways
- Autophagy in Disease and Therapy
- Cancer Mechanisms and Therapy
- Bioactive Compounds and Antitumor Agents
- Cell death mechanisms and regulation
- Phytochemical compounds biological activities
- Pharmacological Effects of Natural Compounds
- Ion channel regulation and function
- Medicinal Plants and Bioactive Compounds
- Cancer, Lipids, and Metabolism
- RNA Research and Splicing
- Berberine and alkaloids research
- Endoplasmic Reticulum Stress and Disease
China Medical University
2016-2025
Guangxi University
2021-2024
Nanjing University of Chinese Medicine
2020-2023
China Academy of Chinese Medical Sciences
2023
State Administration of Traditional Chinese Medicine of the People's Republic of China
2023
Fujian Medical University
2018-2021
Union Hospital
2018-2021
Zhejiang University
2020
Jiangsu Provincial Academy of Traditional Chinese Medicine
2020
Southeast University
2020
Abstract Genistein, a major isoflavone compound in soybeans, has been shown to have biological activities including anti‐cancer activates. In the present, we investigated anti‐leukemia activity of genistein on HL‐60 cells vitro. The percentage viable cell, cell cycle distribution, apoptotic death, reactive oxygen species (ROS), and Ca 2+ production level Δ Ψ m were measured by flow cytometric assay. Cell apoptosis endoplasmic reticulum (ER) stress associated protein expressions examined...
Benzyl isothiocyanates (BITC), a member of the isothiocyanate (ITC) family, inhibits cell growth and induces apoptosis in many types human cancer lines. The present study investigated mechanisms underlying BITC-induced A375.S2 melanoma cells. To observe morphological changes viability, flow cytometric assays, counting, contrast-phase microscopic examination were carried out cells after BITC treatment. Cell cycle distribution assessed with analysis by DAPI staining, propidium iodide (PI),...
P53 family members with a transactivation domain induce cell cycle arrest and promoteapoptosis. However, ΔNp63 isotypes lacking the (TA)- promote cellproliferation tumorigenesis in vitro vivo. Although p53, TAp63 or TAp73 are stabilizedupon DNA damage, we found that genotoxic stress agents induced dramatic decrease andphosphorylation of ΔNp63α squamous carcinoma cells. Further work revealed RACK1physically associated p63α C-terminal through its WD40 domain....
Sulforaphane (SFN), an isothiocyanate, exists exclusively in cruciferous vegetables, and has been shown to possess potent antitumor chemopreventive activity. However, there is no available information that shows SFN affecting human colon cancer HCT 116 cells. In the present study, we found induced cell morphological changes, which were photographed by contrast-phase microscopy, decreased viability. also G2/M phase arrest apoptosis cells, measured with flow cytometric assays. Western blotting...
To investigate the effects of ellagic acid on growth inhibition TSGH8301 human bladder cancer cells in vitro, were incubated with various doses for different time periods. The phase-contrast microscope was used examining and photographing morphological changes cells. Flow cytometric assay to measure percentage viable cells, cell cycle distribution, apoptotic ROS, mitochondrial membrane potential (ΔΨm), Ca2+, caspase-9 -3 activities after exposure acid. Western blotting examine apoptosis...
The effects of two-drug combination, all-trans retinoic acid (ATRA) and bisdemethoxycurcumin (BDMC), on apoptosis induction liver cancer cells were investigated in human Hep 3B cells. Two-drug combination caused a more effective decrease cell viability S phase arrest, DNA damage, than that ATRA or BDMC only. Also, the to undergo significantly increased ROS productions when compared Results Western blotting demonstrated expressions Fas, pro-apoptotic proteins, active form caspase-3 -9, but...
p63, a p53 family member, is required for craniofacial and limb development as well proper skin differentiation. However, p63 mutations associated with the ankyloblepharon-ectodermal dysplasia-clefting (AEC) syndrome (Hay-Wells syndrome) were found in carboxyl-terminal region sterile α-motif. By two-hybrid screen we identified several proteins that interact p63α carboxyl terminus its α-motif, including apobec-1-binding protein-1 (ABBP1). AEC-associated completely abolished physical...
Lung cancer is one of the leading causes death in cancer-related diseases. Cantharidin (CTD) components natural mylabris (Mylabris phalerata Pallas). Numerous studies have shown that CTD induced cytotoxic effects on cells. However, there no report to demonstrate apoptosis human lung Herein, we investigated effect cell via induction H460 Flow cytometry assay was used for examining percentage viability, sub-G1 phase cycle, reactive oxygen species (ROS) and Ca2+ productions levels mitochondrial...
Bufalin has been shown to be effective against a variety of cancer cells, but its role in lung never studied an animal model. In this study, we evaluated bufalin effects human cell line NCI-H460 both vitro and vivo. caused significant cytotoxicity cells at concentration as low 1 μM. DNA condensation was observed bufalin-treated dose-dependent manner. Mitochondrial membrane potential (ΔΨm ) reduced reactive oxygen species (ROS) were increased cells. Levels several proapoptotic proteins such...
One anticancer strategy suggests targeting mitochondrial metabolism to trigger cell death through slowing down energy production from the Warburg effect.Fenofibrate is a clinical lipid-lowering agent and an effective drug.In present study, we demonstrate that fenofibrate provided novel mechanisms for delaying oral tumor development via reprogramming of metabolic processes.Fenofibrate induced cytotoxicity by decreasing oxygen consumption rate (OCR) was accompanied with increasing...
Radiotherapy is one of the most common treatment options for local or regional advanced prostate cancer (PCa). Importantly, PCa prone to radioresistance and often develops into malignancies after long-term radiotherapy. Antrocin, a sesquiterpene lactone isolated from Antrodia cinnamomea, possesses pharmacological efficacy against various types; however, its therapeutic potential requires comprehensive exploration, particularly in radioresistant cells. In this study, we emphasized effects...
Cantharidin (CTD), a component of natural mylabris (Mylabris phalerata Pallas), has been shown to have biological activities and induce cell death in many human cancer cells. In the present study, we investigated effect CTD on migration invasion NCI-H460 lung Cell viability was examined results indicated that decreased percentage viable cells dose-dependent manners. inhibited Gelatin zymography analysis used measure matrix metalloproteinases (MMP-2/-9) enzymatic MMP-2/-9 Western blotting...
Metabolic reprogramming plays a crucial role in the development of cancer. The aim this study was to explore effect fenofibrate, an agonist peroxisome proliferator-activated receptor alpha (PPARα), on gene expression profiles mitochondrial energy metabolism. Our results showed that PPARα negatively correlated with tumor progression oral cancer mouse model. Activation through fenofibrate suppressed migration cells. Differential protein profiling demonstrated expressions genes related...
Abstract Background Endometrial carcinoma is one of the most common gynecological malignancies in women. The diagnosis disease at early or premalignant stages crucial for patient's prognosis. To date, and follow-up endometrial hyperplasia require invasive procedures. Therefore, there considerable demand identification biomarkers to allow non-invasive detection these conditions. Methods In this study, we performed a quantitative proteomics analysis on serum samples from simple hyperplasia,...
TOC summary SGK1/GRASP-1/Rab4 participated in complete Freund’s adjuvant-induced inflammatory pain via regulating GluR1-containing AMPAR recycling at the dorsal horn neurons. The elusiveness of mechanism underlying is a major impediment developing effective clinical treatments. We examined whether phosphorylation spinal serum- and glucocorticoid-inducible kinase 1 (SGK1) downstream glutamate receptor interacting protein (GRIP)-associated protein-1 (GRASP-1)/Rab4-dependent...
Norcantharidin (NCTD) is one of the ingredients blister beetles which have been used in Chinese medicine for a long time. The purpose this study was to investigate inhibitory effects NCTD on TSGH 8301 human bladder cancer cells vitro and mechanisms through it exerts its anticancer action. Cell morphological analysis performed using phase-contrast microscope. percentage viable cells, cell cycle distribution, sub-G1 phase (apoptosis), reactive oxygen species (ROS) production levels...
Many studies have demonstrated that berberine inhibited the cell migration and invasion in human cancer lines. However, exact molecular mechanism of inhibiting melanoma A375.S2 A375.S2/PLX (PLX4032 induced resistant A375.S2) skin cells remains unknown. In this study, we investigated anti-metastasis mechanisms vitro. Berberine at low concentrations (0, 1, 1.5 2 μM) morphological changes reduced viable number mobility, migration, were assayed by wound healing transwell filter. The gelatin...
ABSTRACT Sulforaphane (SFN), one of the isothiocyanates, is a biologically active compound extracted from cruciferous vegetables, and has been shown to induce cytotoxic effects on many human cancer cells including leukemia cells. However, exact molecular mechanism altered gene expression associated with apoptosis unclear. In this study, we investigated SFN‐induced whether or not they went through cell‐cycle arrest induction further examined in HL‐60 Cell viability, distribution, sub‐G1...
The increasing genomic complexity of acute myeloid leukemia (AML), the most common form leukemia, poses a major challenge to its therapy. To identify potent therapeutic targets with ability block multiple cancer-driving pathways is thus imperative. unique peptidyl-prolyl cis-trans isomerase Pin1 has been reported promote tumorigenesis through upregulation numerous pathways. Although key drug target for treating promyelocytic (APL) caused by fusion oncogene, much less known about role in...
p63 mutations have been identified in several developmental abnormalities, including splithand/foot malformation (SHFM). In this study, we demonstrate that the C-terminal domain of p63αassociates with E2 ubiquitin conjugating enzyme, Ubc9. A p63α mutation, Q634X, whichnaturally occurs SHFM modulated interaction Ubc9 yeast genetic assay.Furthermore, catalyzed conjugation small modifier-1 (SUMO-1),which covalently modified vitro and vivo at two positions (K549E K637E), eachsituated...
Cantharidin (CTD), a component of natural mylabris (Mylabris phalerata Pallas) was reported to have high cytotoxicity in many human cancer cell lines. However, it not affect melanoma A375.S2 cells. In the present study, we found that CTD induced morphological changes and decreased percentage viable cells G2/M phase arrest induction apoptosis Results also showed generation reactive oxygen species (ROS) Ca2+ mitochondria membrane potential lead release cytochrome c, AIF Endo G. Further...
Abstract T-complex protein 1 (TCP1) is one of the subunits chaperonin-containing T complex (CCT), which involved in folding, cell proliferation, apoptosis, cycle regulation, and drug resistance. Investigations have demonstrated that TCP1 a factor being responsible for resistance breast ovarian cancer. However, role acute myeloid leukemia (AML) remains elusive. In present study, we discovered expression was elevated AML patients high associated with low complete response rate along poor...