Bone cancer is one of the cancer-related diseases, and there are increased numbers patients with bone worldwide. Therefore efficacy treatment considered extremely vital. Bufalin has been showed to have biological activities including anticancer in vitro vivo. However, exact associated mechanisms for bufalin induced apoptosis human cells still unclear. In present study, we investigated effect on cytotoxic effects U-2 OS osteosarcoma cells. For examining apoptotic cell deaths, used flow...

Eur J Clin Invest 2011; 41 (3): 277–284 Background Interleukin-6 (IL-6) promotes proliferation and invasion in colorectal carcinoma, serum IL-6 levels are correlated with survival patients carcinoma. In this study, we attempted to clarify the signal pathway downstream of role receptor complex terms biological effects clonogenic growth invasiveness carcinoma cells. Materials methods IL-6-stimulated SW480 cells were treated neutralization antibody, mitogen-activated protein kinase (MAPK)...

ABSTRACT Sulforaphane (SFN), one of the isothiocyanates, is a biologically active compound extracted from cruciferous vegetables, and has been shown to induce cytotoxic effects on many human cancer cells including leukemia cells. However, exact molecular mechanism altered gene expression associated with apoptosis unclear. In this study, we investigated SFN‐induced whether or not they went through cell‐cycle arrest induction further examined in HL‐60 Cell viability, distribution, sub‐G1...

Studies have shown an association between lower urinary tract symptoms (LUTS) and increased risk of dementia. Whether anticholinergic use contributes to the development dementia in patients with LUTS remains unknown, especially Asian populations. This study aims investigate LUTS.This included aged 50 years over newly diagnosed (January 2001 December 2005), divided into four groups according their cumulative defined daily doses (cDDDs) anticholinergics: < 28 cDDDs, 28-84 85-336 ≥337 cDDDs....

Oral cancer has been reported to be one of the major cancer-related diseases in human populations and treatment oral is still unsatisfied. Fisetin, a flavonoid from plants several biological activities such as antioxidant, anti-inflammatory anticancer function, but its cytotoxicity cells unknown. In present study, we investigated fisetin-induced cytotoxic effects on HSC3 vitro. Materials Methods/Results: We used flow cytometric assay show fisetin induced apoptotic cell death through...

Cardamonin, the chalcone class, is one of natural components from spicy herbaceous plant (Alpinia conchigera Griff) and has anticancer activities in many human cancer cell lines. There is, however, no information to show that cardamonin induces apoptosis alters associated gene expressions mouse leukemia cells. Thus, we investigated effects on apoptotic death expression WEHI-3 cells vitro. Results indicated decreased total viable number via induced morphological changes were assay by...

Sulforaphane (SFN) is an isothiocyanate, inducing cytotoxic effects in various human cancer cells, including leukemia cells through cell cycle arrest and apoptosis. However, the effect of SFN on immune responses a mouse model remains to be investigated. The present study investigated whether has WEHI‑3‑induced vivo. Normal BALB/c mice were injected with WEHI‑3 generate model, subsequently treated placebo or (0, 285, 570 1,140 mg/kg) for 3 weeks. Following treatment, all weighted blood...

Abstract Human glioblastoma (GBM) is one of the common cancer death in adults worldwide, and its metastasis will lead to difficult treatment. Finding compounds for future develop treatment urgent. Bisdemethoxycurcumin (BDMC), a natural product, was isolated from rhizome turmeric ( Curcuma longa ), which has been shown against many human cells. In present study, we evaluated antimetastasis activity BDMC GBM Cell proliferation, cell viability, cellular uptake, wound healing, migration...

We compared the measurement of propofol concentrations in plasma or whole blood by high-performance liquid chromatography (HPLC) to that gas (GC). Blood samples were collected from patients who had received bolus injection continuous infusion propofol. The results showed two methods correlated well both and samples. However, significant biphasic differences between specimens observed group. Differences larger This discrepancy resulted clearance propofol, lag redistribution across cell...

Indole-3-carbinol (I3C), a potential anticancer substance, can be found in cruciferous (cabbage family) vegetables, mainly cauliflower and Chinese cabbage. However, the bioactivity of I3C on apoptotic effects murine leukemia WEHI-3 cells promotion immune responses mice model are unclear. In this study, we investigated effect cell-cycle arrest apoptosis vitro immunomodulation vivo. decreased viable caused morphological changes concentration- time-dependent manner. also led to G0/G1 phase...

Abstract Allyl isothiocyanate (AITC) is abundant in cruciferous vegetables and it present pharmacological activity including anticancer many types of human cancer cells vitro vivo. Currently, no available information to show AITC affecting DNA damage repair‐associated protein expression gastric cells. Therefore, the studies, we investigated AITC‐induced cytotoxic effects on AGS SNU‐1 whether or not via induction affected repair associated poteins expressions vitro. Cell viability...

Background/Aim: Laminarin, mainly found in the fronds of Laminaria, has antimicrobial characteristics and induces immune responses. However, there are no available information to show laminarin effect on glutamic oxaloacetic transaminase (GOT) pyruvic (GPT) levels mice with leukemia vivo. Materials Methods: Fifty normal BALB/c were separated randomly into five groups. Group I received diet as control. Leukemia was generated groups II-V using WEHI-3 cells: II positive control; group III, IV V...

Ouabain, a cardiotonic steroid, was used for the treatment of heart failure and atrial fibrillation induces cancer cell apoptosis in many human cells including leukemia cells. However, there are no reports to show effects on immune responses mouse model. In this study, WEHI-3 generated mice were developed treated by oral ouabain at 0, 0.75, 1.5, 3 mg/kg 15 days. Results indicated that did not affect body appearance, but decreased liver spleen weights, B- T-cell proliferation all three doses...

Background/Objectives Studies have shown a strong relationship between depression and dementia. Lower urinary tract symptoms (LUTS) were reported to be independently associated with However, the cognitive dysfunction in patients LUTS is not well characterized. Method We conducted matched cohort study by using one-million population-based dataset Taiwan. A total of 15,944 aged 50 or older included from 2001 2005 followed up until their death end 2012. During follow-up period, 1958 cases...

Background/Aim: Demethoxycurcumin (DMC), one of the derivatives curcumin, has been shown to induce apoptotic cell death in many human cancer lines. However, there is no available information on whether DMC inhibits metastatic activity glioblastoma cells vitro. Materials and Methods: at 1.0-3.0 μM significantly decreased proliferation GBM 8401 cells; thus, we used 2.0 for further investigation regarding anti-metastatic cells. Results: The internalized amount reached highest level after 3 h...

The complete 267 amino acid sequence of abrin‐a B chain was determined by analysis peptides obtained digestion with trypsin, chymotrypsin, lysyl endopeptidase, Staphylacaccus aureus V8 protease and thermolysin. is not identical that predicted previously nucleotide sequencing, indicating the presence isoforms abrin. Comparison ricin‐D reveals a high degree identity (59%). Abrin‐a appears to consist two domains, each domain subdomains (α, β, γ) about 40 residues.

Background/Aim: Bufalin, bufadienolide present in Chan Su, has been shown to induce cancer cell apoptosis many human cells, including leukemia but its effects on immune responses are unknown. Materials and Methods: This study investigated whether bufalin affected of mice with WEHI-3 cell-generated vivo. BALB/c were intraperitoneally injected cells develop then treated oral treatment at different doses (0, 0.1, 0.2 0.4 mg/kg) for 2 weeks. At the end treatment, all weighted blood was...