A5053297356- Allergic Rhinitis and Sensitization
- Food Allergy and Anaphylaxis Research
- Toxin Mechanisms and Immunotoxins
- Galectins and Cancer Biology
- Transgenic Plants and Applications
- Helicobacter pylori-related gastroenterology studies
- Contact Dermatitis and Allergies
- Asthma and respiratory diseases
- Retinal Diseases and Treatments
- Retinal and Optic Conditions
- Glycosylation and Glycoproteins Research
- Heat shock proteins research
- Veterinary medicine and infectious diseases
- Peptidase Inhibition and Analysis
- Cancer, Hypoxia, and Metabolism
- Biochemical and Structural Characterization
- Retinal Imaging and Analysis
- Ubiquitin and proteasome pathways
- S100 Proteins and Annexins
- RNA modifications and cancer
- Redox biology and oxidative stress
- Signaling Pathways in Disease
- Plant Virus Research Studies
- Atherosclerosis and Cardiovascular Diseases
- Venomous Animal Envenomation and Studies
Vision Australia
2024
ACT Government
2024
Centre Hospitalier de Saint-Brieuc
2024
CHU Dijon Bourgogne
2024
Canberra Hospital
2024
Centre for Eye Research Australia
2024
National Taiwan University
2014-2023
The University of Sydney
2018
National Taiwan University Hospital
2002-2017
John Radcliffe Hospital
2012
Shellfish are a common cause of adverse food reactions in hypersensitive individuals and shrimp is one the most frequently reported causes allergic reactions. A novel allergen from Penaeus monodon, designated Pen m 2, was identified by two-dimensional immunoblotting using sera subjects with allergy, followed matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis peptide digest. This then cloned amino acid sequence deduced cDNA sequence. The encoded 356-aa...
Understanding the mechanism by which cell growth, migration, polyploidy, and tumorigenesis are regulated may provide important therapeutic strategies for cancer therapy. Here we identify Skp2–macroH2A1 (mH2A1)–cyclin-dependent kinase 8 (CDK8) axis as a critical pathway these processes, deregulation of this is associated with human breast progression patient survival outcome. We showed that mH2A1 new substrate Skp2 SCF complex whose degradation promotes CDK8 gene protein expression....
Hepatocellular carcinoma (HCC) is one of the leading causes cancer-related death throughout world. Although hepatitis B or C viral infections are main risk factors for HCC, molecular mechanisms to HCC formation have not been clarified. To reduce mortality and improve effectiveness therapy, it important search changes in tumor-specific biomarkers whose function may involve disease progression which be useful as potential therapeutic targets. In this study, we employed two-dimensional...
Abstract Although cockroaches are known to produce allergens that can cause IgE‐mediated hypersensitivity reactions, including perennial rhinitis and asthma, the various cockroach have not yet been fully studied. Many proteins from German show high IgE reactivity, but never comprehensively characterized. To identify these potential allergens, were separated by 2‐DE IgE‐binding analyzed nanoLC‐MS/MS or N ‐terminal sequencing analysis. Using a combination of proteomic techniques bioinformatic...
Although the significant risk factors for hepatocellular carcinoma (HCC) are well known from epidemiological studies, diagnosis of this disease at an early stage is difficult, and HCC remains one leading causes cancer death worldwide. Thus, to identify any useful HCC-related biomarkers still a need. We performed SELDI-TOF MS differentially expressed proteins in serum using weak cation exchange protein chips. Protein characterization was by 2-DE separation nano flow LC-MS/MS. A total 55 sera...
Abstract Allergenic serine proteases are important in the pathogenesis of asthma. One these, Pen c 13, is immunodominant allergen produced by Penicillium citrinum. Many induce cytokine expression, but whether 13 does so human respiratory epithelial cells not known. In this study, we investigated caused IL-8 release and activated protease-activated receptors (PARs) airway cells. airway-derived A549 normal cells, induced a dose-dependent manner. also increased time-dependent manner cleaved...
purpose. Bevacizumab is a potent recombinant humanized monoclonal antibody directed against vascular endothelial growth factor (VEGF). The purpose of this study was to evaluate the therapeutic effect subconjunctival injection bevacizumab on corneal neovascularization (NV) in different rabbit models. methods. Several models NV were used, including (1) micropocket assay with VEGF pellet, (2) basic fibroblast (b-FGF) pellets, (3) mechanical limbal injury–induced NV, and (4) an alkali-induced...
Quercetin, a flavonoid abundantly present in plants, is widely used as phytotherapy prostatitis and prostate cancer. Although quercetin has been reported to have number of therapeutic effects, the cellular target(s) responsible for its anti-cancer action not yet clearly elucidated. Here, employing affinity chromatography mass spectrometry, we identified heterogeneous nuclear ribonucleoprotein A1 (hnRNPA1) direct target quercetin. A specific interaction between hnRNPA1 was validated by...
Sorafenib has become the standard therapy for patients with advanced hepatocellular carcinoma (HCC). Unfortunately, most eventually develop acquired resistance. Therefore, it is important to identify potential biomarkers that could predict efficacy of sorafenib. To target proteins associated development sorafenib resistance, we applied stable isotope labelling amino acids in cell culture (SILAC)-based quantitative proteomic approach analyze differences protein expression levels between...
Abstract Helicobacter pylori (H. ) infection is a major cause of chronic gastritis and highly related to duodenal ulcer (DU) gastric cancer (GC). To identify H. -related GC biomarkers with high seropositivity in patients, differences levels protein expression between from DU patients were analyzed by isobaric tag for relative absolute quantitation (iTRAQ). In total, 99 proteins showed increased (>1.5-fold) compared 40 these categorized KEGG pathway. The four human disease-related adhesin...
The NS5A protein of the hepatitis C virus (HCV) is an integral component viral replicase. It also modulates cellular signaling and perturbs host interferon responses. multifunctional characteristics are mostly attributed to its ability interact with various proteins. This study aimed identify novel factors that decipher significance this interaction in replication. NS5A-interacting proteins were purified by tandem affinity purification (TAP) procedure from cells expressing identified mass...
Alternative splicing involves differential exon selection of a gene transcript to generate mRNA and protein isoforms with structural functional diversity. Abnormal alternative has been shown be associated malignant phenotypes cancer cells, such as chemo-resistance invasive activity. Screening small molecules drugs for modulating RNA in human hepatocellular carcinoma cell line Huh-7, we discovered that amiloride, distinct from four pH-affecting amiloride analogues, could "normalize" the...
Purpose To evaluate the effects and underlying mechanisms of early late subconjunctival injection bevacizumab on inhibition corneal neovascularization (NV). Methods Corneal NV was induced by closed eye contact lens wear followed a silk suture tarsorrhaphy in rabbits. Weekly injections (5.0 mg) for 1 month were started immediately (early treatment group) or after induction with continuous (late group). The severity evaluated. Immunostaining used to intracorneal diffusion bevacizumab,...
The cellular endosomal sorting complex required for transport (ESCRT) was recently found to mediate important morphogenesis processes at the nuclear envelope (NE). We previously showed that Epstein-Barr virus (EBV) BFRF1 protein recruits ESCRT-associated Alix modulate NE structure and promote EBV egress. Here, we uncover new factors mechanisms involved in this process. BFRF1-induced vesicles are similar those observed following reactivation. is ubiquitinated, elimination of possible...
Hepatocellular carcinoma is one of the most common cancer types worldwide. In cases advanced-stage disease, sorafenib considered treatment choice. However, resistance to remains a major obstacle for effective clinical application. Based on integrated phosphoproteomic and The Cancer Genome Atlas (TCGA) data, we identified transcription factor, Y-box binding protein-1 (YB-1), with elevated phosphorylation Ser102 in sorafenib-resistant HuH-7R cells. Phosphoinositide-3-kinase (PI3K) protein...
Helicobacter pylori is an important risk factor of gastric cancer (GC). Although many H. virulence factors have been reported, the pathogenic mechanism by which infection causes GC remains unclear. The aims this study were to identify GC-related antigens from and characterize their roles in development GC. As duodenal ulcer (DU) are considered clinically divergent, we compared two-dimensional immunoblots acid-glycine extract probed with serum samples 15 patients DU find antigens,...
Glioblastoma multiforme is the most common and aggressive glial tumor with poor prognosis. Importantly, effective treatment options for glioblastoma are unmet needs. Obesity low physical activity have been linked a high risk of cancer, exercise related to delayed cancer development progression. Epidemiological studies revealed correlation between survival rate patients glioblastoma. Nevertheless, mechanisms by which exerts its anticancer effects in remain unclear. Here, we found that irisin,...
Cancer cachexia, occurring in ~ 80% pancreatic cancer (PC) patients overall, is a paraneoplastic syndrome mediated by cancer-induced systemic inflammation and characterized weight loss skeletal muscle wasting. Identifying clinically relevant PC-derived pro-inflammatory factors with cachexigenic potential may provide novel insights therapeutic strategies.Pro-inflammatory PC were identified bioinformatic analysis. The abilities of selected candidate inducing atrophy investigated. Expression...
Abstract The combining site of the nontoxic carbohydrate binding protein (Abrus precatorius agglutinin, APA) purified from needs Abrus (Jequirity bean), was studied by quantitative precipitin and precipitin-inhibition assays. Of 26 glycoproteins polysaccharides tested, all, except sialic acid-containing desialized ovine salivary glycoproteins, reacted strongly with lectin, precipitated over 70% lectin added, indicating that APA has a broad range affinity recognizes (internal) Gal beta...
Previous studies have demonstrated that valosin-containing protein (VCP) is associated with H. pylori-induced gastric carcinogenesis. By identifying the interactome of VCP overexpressed in AGS cells using a subtractive proteomics approach, we aimed to characterize cellular responses mediated by and its functional roles pylori-associated cancer. immunoprecipitations followed proteomic analysis identified 288 putative interacting proteins, 18 VCP-binding proteins belonged PI3K/Akt signaling...