Disruption of circadian rhythm may be a risk factor in the development breast cancer, but molecular changes controlled genes cancer cells are still unexplored. We used immunohistochemical staining, methylation specific PCR and direct sequencing methods to analyze three most important genes, namely PER1 , PER2 PER3 55 cases Taiwanese women. Our results reveal disturbances expression period ( PER ) (>95%) cancerous comparison with nearby non-cancerous cells. The gene deregulation is not caused...

Spinal muscular atrophy (SMA) is an autosomal recessive disease characterized by degeneration of the anterior horn cells spinal cord, leading to paralysis with atrophy. No effective treatment this disorder presently available. Studies correlation between severity and amount survival motor neuron (SMN) protein have shown inverse relationship. We report that sodium butyrate effectively increases exon 7-containing SMN in SMA lymphoid cell lines changing alternative splicing pattern 7 2 gene. In...

Spectrin is the major molecular consituent of red cell membrane skeleton. We have isolated overlapping human erythroid beta-spectrin cDNA clones and determined 6773 base pairs contiguous nucleotide sequence. This includes entire coding sequence beta-spectrin. The translates into a 2137 amino acid, 246-kDa peptide. beta-Spectrin found to consist three distinct domains. Domain I, at N terminus, 272-amino acid region lacking resemblance spectrin repetitive motif. Sequences in this exhibit...

Abstract Circadian rhythm plays an important role in the regulation of digestive system. The human circadian is controlled by at least nine genes. aims this study are to understand expression genes between hepatocellular carcinoma tissues and nontumor tissues, explore possible mechanism(s) contributing difference. We analyzed differential 9 46 paired noncancerous real‐time quantitative RT‐PCR immunohistochemical detection. also tested regulatory direct sequencing methylation PCR analysis....

The pathogenesis of multiple myeloma involves complex genetic and epigenetic events. This study aimed to investigate the role clinical relevance long non-coding RNA (lncRNA), metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) in myeloma.Bone marrow mononuclear cells were collected for analysis. samples taken from 45 patients at diagnosis, 61 post-treatment, 18 who relapsed or had progression. Control 20 healthy individuals. Real-time quantitative reverse transcription polymerase...

Long noncoding RNAs (lncRNAs) play crucial roles in tumorigenesis, and lncRNA taurine-upregulated gene 1 (TUG1) has been proven to be associated with several human cancers. However, the mechanisms of TUG1-involved regulation remain largely unknown. We examined expressions TUG1 a cohort 89 patients non-small cell lung cancer (NSCLC) determine association between expression clinical parameters. used circular chromosome conformation capture (4C) coupled next-generation sequencing explore genome...

Abstract The development of endometrial carcinoma (EC) is a multiple‐step process, which includes inactivation tumour suppressor genes, activation oncogenes, and disturbance cancer‐related genes. Recent studies have shown that the circadian cycle may influence cancer prognosis. In this study, expression gene, PER1 , was examined in 35 ECs paired non‐tumour tissues by real‐time quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) immunohistochemistry. Expression levels were...

Abstract Although many genome-wide association studies (GWASs) of hyperuricemia or gout have been reported, the related genetic factors and mechanisms from to gouty attack remain unclear. This study aimed identify pathogenesis by (GWAS). 747 patients, 2071 age-matched controls were recruited analyzed with Affymetrix 650 K chip find variants. The functions genes investigated in an endothelial cell (EC) urate crystal stimulation. GWAS results showed 36 SNPs be strongly associated compared (all...

Monocytes/macrophages are important in orchestrating inflammatory responses. However, knowledge of the long noncoding RNA (lncRNA) regulation monocytic cell differentiation and diseases remains limited. We aimed to elucidate role 17 kb lncRNA transcript T cells (NTT) monocyte functions. Knockdown chromatin immunoprecipitation (ChIP) assays THP-1 (human leukemia line) revealed that NTT is regulated by key transcription factor C/EBPβ it binds promoter nearby gene PBOV1 via hnRNP-U....

Abstract Background Precision therapy for lung cancer requires comprehensive genomic analyses. Specific effects of targeted therapies have been reported in Asia populations, including Taiwanese, but studies rarely performed these populations. Method We enrolled 72 patients with non-small cell cancer, whom 61 had adenocarcinoma, 10 squamous carcinoma, and 1 combined adenocarcinoma carcinoma. Whole-exome or gene sequencing was performed. To identify trunk mutations, we whole-exome two tumor...

BACKGROUND/AIMS: Host genetic factor and hepatic fibrosis may predispose to risk for hepatocellular carcinoma (HCC). This study aimed assess the association between tumor necrosis (TNF) α polymorphism fibrosis, HCC. METHODS: One hundred eight pairs of gender-matched age-matched patients with HCC unrelated healthy controls were genotyped TNF308.2 TNF238.2 alleles polymerase chain reaction direct sequencing. RESULTS: The frequency TNF308.1/TNF308.2 genotype in cases was higher than that [odds...

Sprouty protein is a novel feedback regulator involved in downstream inactivation of several growth factor receptor pathways. Sprouty2 (Spry2) was shown to be downregulated human cancers. High levels microRNA-21 (miRNA-21) expression have been associated with poor survival and response adjuvant chemotherapy cancer patients. But the effect Spry2 colon remained unknown. Paired tumor normal mucosa samples from patients were examined for their mRNA miRNA-21 by real-time quantitative RT-PCR...

Pseudogenes, especially those that are transcribed, may not be mere genomic fossils, but their biological significance remains unclear. Postulating in the human genome, as animal models, pseudogenes function gene regulators through generation of endo-siRNAs (esiRNAs), antisense RNAs or RNA decoys, we performed bioinformatic and subsequent experimental tests to explore esiRNA-mediated mechanisms pseudogene involvement oncogenesis. A genome-wide survey revealed a partial retrotranscript ψPPM1K...

The antihypertensive drug amiloride is being considered as a tactic to improve cancer therapy including that for chronic myelogenous leukemia. In this study, we show modulates the alternative splicing of various genes, Bcl-x, HIPK3, and BCR/ABL, effect not mainly related pH alteration, which known drug. Splice modulation involved factors, with phosphorylation state serine-arginine-rich (SR) proteins also altered during process. Pretreatment okadaic acid inhibit protein phosphatase PP1...

The aim of the present study was to identify genomic alterations in Taiwanese endometrial cancer patients. This information is vitally important Taiwan, where second most common gynecological cancer. We performed whole-exome sequencing on DNA from 14 tumor tissue samples used Genome Analysis Tool kit software package for data analysis, and dbSNP, Catalogue Somatic Mutations Cancer (COSMIC) Atlas (TCGA) databases comparisons. Variants were validated via Sanger sequencing. identified 143...