A5045333057- Cancer-related gene regulation
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Lung Cancer Treatments and Mutations
- Cancer-related molecular mechanisms research
- Genetic factors in colorectal cancer
- Glycosylation and Glycoproteins Research
- Cancer Research and Treatments
- Skin and Cellular Biology Research
- RNA Research and Splicing
- Protease and Inhibitor Mechanisms
- Cancer, Lipids, and Metabolism
- Kruppel-like factors research
- Cancer Genomics and Diagnostics
- Peptidase Inhibition and Analysis
- Circadian rhythm and melatonin
- Cell death mechanisms and regulation
- Molecular Biology Techniques and Applications
- Mesenchymal stem cell research
- Medical Imaging and Pathology Studies
- Head and Neck Cancer Studies
- Wnt/β-catenin signaling in development and cancer
- Cell Adhesion Molecules Research
- Colorectal Cancer Treatments and Studies
Changhua Christian Hospital
2016-2025
National Chung Hsing University
2022-2024
Kaohsiung Medical University
2000-2023
Chung Shan Medical University
2014-2023
China Medical University
2001-2014
China Medical University Hospital
2003-2014
Institute of Biomedical Sciences, Academia Sinica
2014
Chang Gung University
2011
National Chung Cheng University
2008-2011
Chang Gung Memorial Hospital
2011
Disruption of circadian rhythm may be a risk factor in the development breast cancer, but molecular changes controlled genes cancer cells are still unexplored. We used immunohistochemical staining, methylation specific PCR and direct sequencing methods to analyze three most important genes, namely PER1 , PER2 PER3 55 cases Taiwanese women. Our results reveal disturbances expression period ( PER ) (>95%) cancerous comparison with nearby non-cancerous cells. The gene deregulation is not caused...
Abstract Circadian rhythm plays an important role in the regulation of digestive system. The human circadian is controlled by at least nine genes. aims this study are to understand expression genes between hepatocellular carcinoma tissues and nontumor tissues, explore possible mechanism(s) contributing difference. We analyzed differential 9 46 paired noncancerous real‐time quantitative RT‐PCR immunohistochemical detection. also tested regulatory direct sequencing methylation PCR analysis....
Cdk1 (cyclin-dependent kinase 1) is critical regulator of the G2-M checkpoint. Cyclin-dependent pathways are considered possible targets for cancer treatment; however, prognostic role in colorectal still controversial. Therefore, we attempted to determine impact on clinical outcome patients further identify its cancer. immunoreactivity was analyzed by immunohistochemistry (IHC) 164 specimens from primary patients. The medium follow-up time after surgery 3.7 years (range: 0.01 13.10 years)....
Abstract The development of endometrial carcinoma (EC) is a multiple‐step process, which includes inactivation tumour suppressor genes, activation oncogenes, and disturbance cancer‐related genes. Recent studies have shown that the circadian cycle may influence cancer prognosis. In this study, expression gene, PER1 , was examined in 35 ECs paired non‐tumour tissues by real‐time quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) immunohistochemistry. Expression levels were...
Abstract Although DNA hypermethylation within promoter CpG islands is highly correlated with tumorigenesis, it has not been established whether a specific tumor suppressor gene (TSG) sufficient to fully transform somatic stem cell. In this study, we addressed question using novel targeted methylation technique methylate the promoters of HIC1 and RassF1A, two well-established TSGs, along two-component reporter system visualize successful targeting human bone marrow–derived mesenchymal cells...
Abstract Background Gastric cancer (GC), an aggressive malignant tumor of the alimentary tract, is a leading cause cancer-related death. Circadian rhythm exhibits 24-hour variation in physiological processes and behavior, such as hormone levels, metabolism, gene expression, sleep wakefulness, appetite. Disruption circadian has been associated with various cancers, including chronic myeloid leukemia, head neck squamous cell carcinoma, hepatocellular endometrial breast cancer. However,...
Abstract Glioblastoma (GBM) is the most mortality brain cancer in world. Due to high invasion and drug resistance cause poor prognosis of GBM. Naringenin, an ingredient citrus, exhibits many cellular functions such as antioxidant, anti‐inflammation, anticancer. Naringenin inhibits migration bladder lung via modulation MMP‐2 and/or MMP‐9 activities, trigger apoptosis gastric cells through downregulation AKT pathway. However, effects naringenin GBM still remain be elucidated. In this study, we...
Changes in mitochondrial DNA (mtDNA) content cancers have been reported with controversial results, probably due to small sample size and variable pathological conditions. In this study, mtDNA 302 breast tumor/surrounding normal tissue pairs were evaluated correlated the clinico-pathological characteristics of tumors. Overall, tumor tissues is significantly lower than that surrounding tissues,<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...
The aim of the present study was to identify genomic alterations in Taiwanese endometrial cancer patients. This information is vitally important Taiwan, where second most common gynecological cancer. We performed whole-exome sequencing on DNA from 14 tumor tissue samples used Genome Analysis Tool kit software package for data analysis, and dbSNP, Catalogue Somatic Mutations Cancer (COSMIC) Atlas (TCGA) databases comparisons. Variants were validated via Sanger sequencing. identified 143...
Abstract Aim Colorectal cancer ( CRC ) is the second commonest cause of death in Taiwan. Although numerous genes have been associated with tumorigenesis colorectal cancer, only a few validated and used as biomarkers for predicting clinical outcome. The aim this study was to analyse association APC gene mutation miR‐21 expression outcome patients. Method In total, 195 patients were enrolled single medical centre between 2003 2007. analysed by direct DNA sequencing real‐time reverse...
Background Cathepsin B (CTSB), a member of the cathepsin family, is cysteine protease that widely distributed in lysosomes cells various tissues. It overexpressed several human cancers and may be related to tumorigenesis. The main purpose this study was analyze CTSB expression oral squamous cell carcinoma (OSCC) its correlation with patient prognosis. Methodology/Principal Findings Tissue microarrays were used detect 280 patients examine association between clinicopathological parameters. In...
Methylation of CpG dinucleotides in the promoter sequence a gene can lead to deregulated and suppressed expression. In this study, we have developed procedures for methylation-specific polymerase chain reaction (MSP) sequencing analysis determine methylation status sequences nine circadian genes 35 endometrial cancers (EC) paired noncancerous tissues. DNA was found PER1, PER2, CRY1, but not other six ECs normal tissues examined. Eleven EC showed or CRY1. Of these 11 cases, 1 had all three...
Abstract Background The roles of mitochondria in energy metabolism, the generation ROS, aging, and initiation apoptosis have implicated their importance tumorigenesis. In this study we aim to establish mutation spectrum understand role somatic mtDNA mutations esophageal cancer. Methods entire mitochondrial genome was screened for 20 pairs (18 squamous cell carcinomas, one adenosquamous carcinoma adenocarcinoma) tumor/surrounding normal tissue cancers, using temporal temperature gradient gel...
To assess the possible correlations between reported candidate genes (VEGFR3, FOXC2, ITGA9 and ITGB1) clinical response in fetuses with severe congenital chylothorax (CC) treated by prenatal OK-432 pleurodesis.We studied 12 unrelated CC, receiving fetal therapy pleurodesis. Genotyping of parameters these were investigated. Additional 96 control individuals enrolled to evaluate polymorphisms at population.A recurrent heterozygous missense mutation (c.1210G>A, p.G404S) was identified...
Tumor angiogenesis is a critical element of cancer progression, and strategies for its selective blockade are still sought. Here, we examine the angiogenic effects Globo-H ceramide (GHCer), most prevalent glycolipid in majority epithelial cancers one that acts as an immune checkpoint. report GHCer becomes incorporated into endothelial cells through absorption microvesicles shed from tumor cells. In cells, addition induces migration, tube formation, intracellular Ca(2+) mobilization vitro...
Although breast phyllodes tumors are rare, there is no effective therapy other than surgery. Little known about their tumor biology. A malignant contains heterologous stromal elements, and can transform into rhabdomyosarcoma, liposarcoma osteosarcoma. These versatile properties prompted us to explore possible relationship mesenchymal stem cells (MSCs) search for the presence of cancer (CSCs) in tumors. Paraffin sections were examined various markers by immunohistochemical staining....
Abstract While aberrant JAK/STAT signaling is crucial to the development of gastric cancer (GC), its effects on epigenetic alterations transcriptional targets remains unclear. In this study, by expression microarrays coupled with bioinformatic analyses, we identified a putative STAT3 target gene, NR4A3 that was downregulated in MKN28 GC daughter cells overexpressing constitutively activated mutant (S16), as compared an empty vector control (C9). Bisulphite pyrosequencing and demethylation...
Overexpression of cyclooxygenase-2 in oral cancer increases lymph node metastasis and is associated with a poor prognosis. The potential celecoxib (CXB) use reported treatment by inhibiting proliferation through apoptosis, but the effects on epithelial-mesenchymal transition (EMT) cell mobility remain unclear. We performed preclinical study population-based to evaluate CXB prevention progression occurrence. in-vitro findings showed that involved inhibition EMT blocking transcription factors...
The purpose of this study was to investigate biomarkers associated with poor clinical outcomes in colorectal cancer (CRC) by utilizing comprehensive datasets from the gene expression omnibus (GEO) databases GSE41258, GSE39582, and GSE44861. We initially identified differentially expressed genes (DEGs) applied weighted co-expression network analysis (WGCNA) GSE41258 dataset reveal key modules CRC. Enrichment analyses were conducted gain insights into underlying biology CRC, particularly...