A5034035020- Endometriosis Research and Treatment
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Reproductive System and Pregnancy
- Uterine Myomas and Treatments
- Epigenetics and DNA Methylation
- Estrogen and related hormone effects
- Protein Tyrosine Phosphatases
- Circular RNAs in diseases
- Fibroblast Growth Factor Research
- Reproductive Physiology in Livestock
- Cancer-related gene regulation
- Endometrial and Cervical Cancer Treatments
- MicroRNA in disease regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- ATP Synthase and ATPases Research
- Histone Deacetylase Inhibitors Research
- Genetic Neurodegenerative Diseases
- Ubiquitin and proteasome pathways
- Inflammatory mediators and NSAID effects
- Phagocytosis and Immune Regulation
- Hidradenitis Suppurativa and Treatments
- Microtubule and mitosis dynamics
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Kruppel-like factors research
National Cheng Kung University
2016-2025
National Chung Cheng University
2011-2025
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2008-2023
National Cheng Kung University Hospital
2023
Institute of Physiology and Basic Medicine
2006-2023
China Medical University
2005-2018
Tainan University of Technology
2005-2018
Baylor College of Medicine
2012-2013
Massachusetts Institute of Technology
2013
Harvard University
2013
Circular RNA (circRNA) is a class of noncoding whose functions remain mostly unknown. Recent studies indicate circRNA may be involved in disease pathogenesis, but direct evidence scarce. Here, we characterize the functional role novel circRNA, circCCDC66, colorectal cancer. RNA-Seq data from matched normal and tumor colon tissue samples identified numerous circRNAs specifically elevated cancer cells, several which were verified by quantitative RT-PCR. CircCCDC66 expression was polyps...
The switch of cellular metabolism from mitochondrial respiration to glycolysis is the hallmark cancer cells and associated with tumor malignancy. However, mechanism this metabolic remains largely unknown. Herein, we reported that hypoxia-inducible factor-1 (HIF-1) induced pyruvate dehydrogenase kinase-3 (PDK3) expression leading inhibition respiration. Promoter activity assay, small interference RNA knockdown chromatin immunoprecipitation assay demonstrated hypoxia-induced PDK3 gene was...
Prostaglandin (PG) F2α is the primary luteolysin in most species. A single treatment with PGF2α will cause regression of mid-cycle but not early-cycle (Days 1-5 after estrus) bovine corpus luteum (CL) despite presence similar concentrations high-affinity receptors (FP receptors). This study was designed to determine whether activated intracellular processes early- and CL. Cows received saline or 25 mg injection (i.m.; n = 6/group) on Day 4 11 onset LH surge (induced by GnRH injection), CL...
The switch of cellular metabolism from mitochondrial respiration to glycolysis is the hallmark cancer cells and associated with tumor malignancy. Pyruvate dehydrogenase kinase-1 (PDK1) PDK3 participate in metabolic cells; however, medical significance PDK1 progression not known. Here, we assessed expression profiles colorectal cancer. Western blot analysis (n = 74) demonstrated that was markedly increased colon compared adjacent normal tissues, whereas decreased cells. In addition,...
Endometriosis is the primary cause of infertility in women, with a prevalence rate ranging from 5% to 10%. Women endometriosis suffer symptoms such as chronic pelvic pain, dysmenorrhea and dyspareunia, which significantly reduce quality life. polygenic disease complex, multifactorial etiology. The mechanism responsible for initiation development this remains largely unknown. Prostaglandin E 2 (PGE ), versatile eicosanoid that exerts numerous physiological pathological functions, has been...
Abstract Context: Aberrant activation of MAPK has been implicated to play important roles in pathological processes endometriosis. However, how are constitutively activated endometriotic tissues remains largely unknown. microRNA small noncoding RNA that regulate the stability or translational efficiency target mRNA by interacting with 3′ untranslated region. Thus, miRNA thought be modulators transcriptional response, fine-tuning gene expression. Objective: The aim this study was evaluate...
Abstract Motivation: Cancer development is a complex and heterogeneous process. It estimated that 5–10% of human genes probably contribute to oncogenesis, whereas current experimentally validated cancer only cover 1% the genome. Thus hundreds may still remain be identified. To search for new play roles in carcinogenesis facilitate research, we developed systematic workflow use information saved previously established tumor-associated gene (TAG) database. Results: By exploiting conserved...
Abstract Although early detection and treatment of prostate cancer (PCa) improves outcomes, many patients still die metastatic PCa. Here, we report that PCa exhibits reduced levels the microRNAsmiR-101 miR-27a. These micro-RNAs (miRNAs) negatively regulate cell invasion inhibit expression FOXM1 CENPF, two master regulators metastasis in Interestingly, repression CENPF by these miRNAs occurs through COUP-TFII, a member orphan nuclear receptors family. Loss miR-101 positively correlates with...
The regulation of steroidogenic acute regulatory protein (StAR) gene expression and the synthesis steroids from cholesterol in ectopic endometriosis tissues were investigated. Peritoneal fluid endometrial collected patients with otherwise healthy women. progesterone 17 beta-E2 concentrations highest early stage compared those advanced normal In concordance profile peritoneal steroids, StAR mRNA greatest implants endometriosis. stage, also greater endometrium. contrast, P450 side-chain...
Aberrant production of cyclooxygenase-2 (COX-2) plays pivotal roles in many pathological processes including tumorigenesis and endometriosis, although the underlying mechanism remains obscure. Herein we report evidence to demonstrate that COX-2 is distinctly regulated by IL-1β normal endometriotic stroma. Ectopic stromal cell at least 100 times more sensitive treatment, compared with its eutopic counterpart. Induction expression endometrial stroma primary due enhancement mRNA stability. In...
Abstract Endometriosis, defined as the growth of endometrial tissues outside uterine cavity, is a severe and complex disease affecting more than 10% women. The aetiology endometriosis unclear but immune dysfunction might be an important factor for its development. natural function system to detect destroy aberrant or abnormal cells. Failure eradicate these cells often results in pathogenesis. We report here that phagocytic ability macrophages reduced peritoneal isolated from women with...
Abstract Although DNA hypermethylation within promoter CpG islands is highly correlated with tumorigenesis, it has not been established whether a specific tumor suppressor gene (TSG) sufficient to fully transform somatic stem cell. In this study, we addressed question using novel targeted methylation technique methylate the promoters of HIC1 and RassF1A, two well-established TSGs, along two-component reporter system visualize successful targeting human bone marrow–derived mesenchymal cells...
Hypoxia inducible factor-1 (HIF-1) is the master transcriptional regulator of cellular response to altered oxygen levels. HIF-1α protein elevated in most solid tumors and contributes poor disease outcome by promoting tumor progression, metastasis, resistance chemotherapy. To date, relationship between HIF-1 these processes, particularly chemoresistance, has remained largely unexplored. Here, we show that expression MAPK-specific phosphatase dual-specificity phosphatase-2 (DUSP2) markedly...
Cancer stem-like cells (CSC) evolve to overcome the pressures of reduced oxygen, nutrients or chemically induced cell death, but mechanisms driving this evolution are incompletely understood. Here, we report that hypoxia-mediated downregulation dual specificity phosphatase 2 (DUSP2) is critical for accumulation CSC in colorectal cancer. Reduced expression DUSP2 led overproduction COX-2-derived prostaglandin E2, which promoted cancer stemness via EP2/EP4 signaling pathways. Genetic and...