A5041975586- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Cancer-related gene regulation
- Genetic factors in colorectal cancer
- Cancer Research and Treatments
- Bladder and Urothelial Cancer Treatments
- Histone Deacetylase Inhibitors Research
- Ovarian cancer diagnosis and treatment
- MicroRNA in disease regulation
- Hippo pathway signaling and YAP/TAZ
- Head and Neck Cancer Studies
- Cancer Cells and Metastasis
- FOXO transcription factor regulation
- Genomics and Chromatin Dynamics
- Cancer Immunotherapy and Biomarkers
- Estrogen and related hormone effects
- Cancer, Lipids, and Metabolism
- Helicobacter pylori-related gastroenterology studies
- Medical Imaging Techniques and Applications
- Immune Cell Function and Interaction
- Gut microbiota and health
- Nuclear Receptors and Signaling
- Brain Metastases and Treatment
- TGF-β signaling in diseases
Salk Institute for Biological Studies
2025
Howard Hughes Medical Institute
2025
Houston Methodist
2025
National Chung Cheng University
2015-2024
Kaohsiung Medical University
2018-2024
NYU Langone Health
2022-2024
BC Centre for Disease Control
2018-2024
The Ohio State University
2005-2023
Buddhist Tzu Chi Medical Foundation
2023
Chiayi Chang Gung Memorial Hospital
2023
The dysregulation of transforming growth factor-β (TGF-β) signaling plays a crucial role in ovarian carcinogenesis and maintaining cancer stem cell properties. Classified as member the ATP-binding cassette (ABC) family, ABCA1 was previously identified by methylated DNA immunoprecipitation microarray (mDIP-Chip) to be lines, A2780 CP70. By microarray, it also found upregulated immortalized surface epithelial (IOSE) cells following TGF-β treatment. Thus, we hypothesized that may involved its...
Objective Therapy-induced tumour microenvironment (TME) remodelling poses a major hurdle for cancer cure. As the majority of patients with hepatocellular carcinoma (HCC) exhibits primary or acquired resistance to antiprogrammed cell death (ligand)-1 (anti-PD-[L]1) therapies, we aimed investigate mechanisms underlying adaptation immune-checkpoint targeting. Design Two immunotherapy-resistant HCC models were generated by serial orthotopic implantation cells through anti-PD-L1-treated...
We report our preliminary outcomes following high-dose image-guided intensity modulated radiotherapy (IG-IMRT) for skull base chordoma and chondrosarcoma.Forty-two consecutive IG-IMRT patients, with either (n = 24) or chondrosarcoma 18) treated between August 2001 December 2012 were reviewed. The median follow-up was 36 months (range, 3-90 mo) in the cohort, 67 15-125) cohort. Initial surgery included biopsy (7% of patients), subtotal resection (57% gross total (36% patients). doses cohorts...
Hepatocellular carcinomas (HCC) exhibit distinct promoter hypermethylation patterns, but the epigenetic regulation and function of transcriptional enhancers remain unclear. Here, our affinity- bisulfite-based whole-genome sequencing analyses reveal global enhancer hypomethylation in human HCCs. Integrative epigenomic characterization further pinpoints a recurrent hypomethylated CCAAT/enhancer-binding protein-beta (C/EBPβ) which correlates with C/EBPβ over-expression poorer prognosis...
Affiliative social connections facilitate well-being and survival in numerous species. Engaging interactions requires positive or negative motivational drive, elicited through coordinated activity across neural circuits. However, the identity, interconnectivity, functional encoding of information within these circuits remains poorly understood. Here, we focus on downstream projections dorsal raphe nucleus (DRN) dopamine neurons (DRN DAT ), which previously implicated motivation alongside an...
Abstract Estrogen imprinting is used to describe a phenomenon in which early developmental exposure endocrine disruptors increases breast cancer risk later adult life. We propose that long-lived, self-regenerating stem and progenitor cells are more susceptible the injury than terminally differentiated epithelial duct. Mammospheres, containing enriched progenitors, were as an system simulate this vitro. Using MeDIP-chip, methylation microarray screening method, we found 0.5% (120 loci) of...
Photodynamic therapy (PDT) stands to benefit from improved approaches real-time treatment monitoring. One method is use activatable photosensitizers that can both induce cell death (via singlet oxygen) and monitor it caspase detection). Here, we report porphyrins as caspase-responsive Forster Resonance Energy Transfer (FRET) acceptors organic fluorophore donors. Compared porphyrin FRET donor constructs, oxygen generation was unquenched prior activation, resulting in more efficient...
Ovarian cancer is the fifth leading cause of death and most deadly gynecological malignancy in women. Epigenetic modifications play an important role regulating gene transcription. Specifically, aberrant promoter hypermethylation has been implicated as a hallmark cancer. In order to identify genes that are differentially methylated ovarian cancer, we performed meDIP-chip various cell lines using Agilent 244K CpG island microarray. One targets, ARNTL which core component circadian clock...
High frequency loss of 3p21.3 region where RASSF1A located was demonstrated in several tumors. We aimed to investigate the methylation status and LOH bladder cancer. Three cancer cell lines, 40 cases TCC 14 paired voided urine samples were subjected analysis. By specific PCR, complete promoter gene detected lines T24 UMUC3. Demethylation treatment re-expressed these 2 lines. Methylation also 47.5% (19/40) but not 6 carcinoma situ (CIS) or normal urothelium samples. For study, 57.9% (11/19)...
Human Disabled-2 (DAB2), is a multi-function signalling molecule that it frequently down-regulated in human cancers. We aimed to investigate the possible tumour suppressor effect of DAB2 nasopharyngeal carcinoma (NPC). studied expression NPC cell lines, xenografts and primary samples. The status promoter methylation was assessed by specific PCR bisulfite sequencing. functional role investigated re-introducing into line C666-1. Decrease or absent transcript observed lines xenografts. Loss...
Oral squamous cell carcinoma (OSCC) constitutes >90% of oral cancers and is the sixth most common malignancy among males worldwide fourth leading cause death due to cancer in Taiwan. However, patients do not receive a diagnosis OSCC until late stages, which have lower survival rate. The use molecular marker analysis identify early-stage would permit optimal timing for treatments consequently prolong survival. aim this study was biomarkers using Illumina GoldenGate Methylation Cancer Panel,...
DNA methylation contributes to tumor formation, development and metastasis. Epigenetic dysregulation of stem cells is thought predispose malignant development. The clinical significance in ovarian tumor-initiating (OTICs) remains unexplored. We analyzed the methylomic profiles OTICs (CP70sps) their derived progeny using a human array. qRT-PCR, quantitative methylation-specific PCR (qMSP) pyrosequencing were used verify gene expression cancer cell lines. status genes was validated...
Forkhead box P3 (Foxp3) is the major transcription factor controlling development and function of regulatory T (Treg) cells. Previous studies have indicated epigenetic regulation Foxp3 expression. Here, we investigated whether deoxyribonucleic acid (DNA) methyltransferase inhibitor 5-aza-2′-deoxycytidine (5-Aza) applied peripherally could modulate central nervous system (CNS) inflammation, by using a mouse experimental autoimmune encephalomyelitis (EAE) model. We found that disease activity...
Transforming growth factor-beta (TGF-β)/SMAD signaling is a key regulatory pathway often dysregulated in ovarian cancer and other malignancies. Although loss of TGF-β–mediated inhibition has been shown to contribute aberrant cell behavior, the epigenetic consequence(s) impaired TGF-β/SMAD on target genes not well established. In this study, we show that TGF-β1 causes normal surface epithelial cells, induction nuclear translocation SMAD4, up-regulation ADAM19 (a disintegrin metalloprotease...
The dissemination of antibiotic resistant bacteria from anthropogenic sources into the environment poses an emerging public health threat. Antibiotic resistance genes (ARGs) and gene-capturing systems such as integron-associated integrase (intI) play a key role in alterations microbial communities spread environment. In order to assess effect activities on watersheds southwestern British Columbia, presence putative was analyzed microbiome agricultural, urban influenced, protected watersheds....
Abstract While aberrant JAK/STAT signaling is crucial to the development of gastric cancer (GC), its effects on epigenetic alterations transcriptional targets remains unclear. In this study, by expression microarrays coupled with bioinformatic analyses, we identified a putative STAT3 target gene, NR4A3 that was downregulated in MKN28 GC daughter cells overexpressing constitutively activated mutant (S16), as compared an empty vector control (C9). Bisulphite pyrosequencing and demethylation...
OBJECT The authors performed a pattern-of-failure analysis, with focus on epidural disease progression, in patients treated postoperative spine stereotactic body radiotherapy (SBRT). METHODS Of the 70 75 spinal metastases (cases) SBRT, there were 26 cases of local recurrence and 25 component progression. Twenty-four had preoperative subsequent this cohort was epidural-specific investigation. Preoperative, postoperative, follow-up MRI scans reviewed, characterized based location according to...
Abstract Aberrant Notch activation has been implicated in multiple malignancies and the identification of NOTCH receptors related pathways is critical for targeted therapy. In this study, we aim to delineate most prominent dysregulated receptor comprehensively reveal its deregulation gastric cancer (GC). four members, NOTCH3 was found uniformly upregulated associated with poor clinical outcomes GC datasets. siRNA-mediated knockdown demonstrated antitumor effects by suppressing cell...
Fermented fruits and vegetables play an important role in safeguarding food security world-wide. Recently, robust sequencing-based microbial community analysis platforms have improved safety assessment. This study aimed to examine the composition of bacteria evaluate bacterial fermented fruit products using high-throughput 16S-rRNA metagenomic analysis. The operational taxonomic unit-based classification DNA sequences revealed 53 genera. However, amplicon sequencing variant (ASV)-based...
Abstract Purpose: High-grade serous ovarian cancer (HGSOC), the most aggressive subtype, has poor outcomes due to late-stage diagnosis and a complex tumor microenvironment (TME). This study investigates exosomal microRNAs (miRNAs) in HGSOC, focusing on their role immune evasion mechanisms within TME potential as biomarkers for early detection therapeutic targets. Methods: We conducted comprehensive miRNA sequencing exosomes derived from nine human cell lines, including those representing...
The purpose of this study was to evaluate the efficacy circle Willis CT angiography with shaded-surface display in detection and assessment suspected intracranial aneurysms.Twenty-three patients who had clinical or radiologic suspicion aneurysms were studied prospectively conventional angiography. images obtained interpreted a double-blind fashion by different radiologists. Conventional angiography, which reference standard study, diagnosed 17 15 patients. usefulness two types for surgical...
Abstract Purpose: Repetitive ribosomal DNA (rDNA) genes are GC-rich clusters in the human genome. The aim of study was to determine methylation status two rDNA subunits, 18S and 28S genes, ovarian tumors correlate levels with clinicopathologic features a cohort cancer patients. Experimental Design: examined by quantitative methylation-specific PCR 74 late-stage cancers, 9 histologically uninvolved, 11 normal surface epithelial samples. In addition, gene expression rDNAs cell lines were...