A5100392923- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Cancer Mechanisms and Therapy
- Cancer-related Molecular Pathways
- Gastric Cancer Management and Outcomes
- Gastrointestinal Tumor Research and Treatment
- Microtubule and mitosis dynamics
- Peptidase Inhibition and Analysis
- Pediatric Hepatobiliary Diseases and Treatments
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Gallbladder and Bile Duct Disorders
- DNA and Nucleic Acid Chemistry
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Genomics, phytochemicals, and oxidative stress
- Pancreatic and Hepatic Oncology Research
- Plant Toxicity and Pharmacological Properties
- Drug-Induced Hepatotoxicity and Protection
- MicroRNA in disease regulation
- Endoplasmic Reticulum Stress and Disease
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer, Lipids, and Metabolism
Southwest Medical University
2022-2025
Sun Yat-sen University
2019-2025
Xuzhou Medical College
2018-2025
Sun Yat-sen Memorial Hospital
2025
Huazhong University of Science and Technology
2019-2025
City of Hope
2019-2025
PowerChina (China)
2024-2025
Wuhan National Laboratory for Optoelectronics
2025
Nanjing Drum Tower Hospital
2015-2024
Beckman Research Institute
2019-2024
Abstract miR-375 is a tumor-suppressive microRNA (miRNA) in gastric cancer (GC). However, its molecular mechanism remains unclear. The aim of this study to comprehensively investigate how involved Hippo pathway by targeting multiple oncogenes. expression cell lines and primary GC was investigated qRT-PCR. regulation YAP1, TEAD4, CTGF evaluated qRT-PCR, western blot, luciferase reporter assays, respectively. functional roles the related genes were examined siRNA-mediated knockdown or ectopic...
The nucleosome is the fundamental unit for packaging genome. A detailed molecular picture its conformational dynamics crucial understanding transcription and gene regulation. We investigate disassembly of single nucleosomes using a predictive coarse-grained protein DNA model with transferable force fields. This quantitatively describes thermodynamic stability both histone core complex predicts rates transient opening that match experimental measurements. Quantitative characterization...
Gastric cancer (GC) is one of the frequent causes cancer-related death in eastern Asian population. IGF2BP2 lists top rank up-regulated genes GC, but its functional role unclear.The expression IGF2BP3 GC cell lines and primary samples was examined by qRT-PCR Western blot. The biological revealed a series vitro studies. Its regulation microRNAs (miRNAs) predicted TargetScan confirmed luciferase assays rescue experiments.IGF2BP3 ranked No.1 microarray analysis lines. level observed tissues...
Protein regulator of cytokinesis-1 (PRC1) belongs to the microtubule-associated proteins (MAPs) family, and is involved in cytokinesis. Recent investigations suggest PRC1 involvement human carcinogenesis, including breast carcinoma, hepatocellular carcinoma etc. However, whether contributes lung adenocarcinoma tumorigenesis remains unknown. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR), Western blotting Immunohistochemical staining (IHC) were used evaluate contrast...
Abstract Background Colon cancer (CC) cells can exhibit stemness and expansion capabilities, which contribute to resistance conventional chemotherapies. Aberrant expression of CBX8 has been identified in many types cancer, but the cause this aberrant whether is associated with properties CC remain unknown. Methods qRT-PCR IHC were applied examine levels normal chemoresistant tissues. Cancer cell chemosensitivity evaluated by spheroid formation, colony Western blot flow cytometry assays....
Polysaccharide-based hydrogels have been developed for many years to treat burn wounds. Essential oils extracted from aromatic plants generally exhibit superior biological activity, especially antibacterial properties. Studies shown that mixed with essential great potential wound healing. This study aimed develop an polysaccharide-based hydrogel oil skin repair.Eucalyptus (EEO), ginger (GEO) and cumin (CEO) were employed the preparation of effective physically crosslinked by carboxymethyl...
Lung cancer is one of the most common malignant cancers worldwide. Searching for specific targets and developing efficient therapies with lower toxicity urgently needed. HPS90 a key chaperon protein that has multiple client proteins involved in development cancer. In this study, we investigated transcriptional levels HSP90 isoforms cancerous normal tissues lung patients datasets. The higher expression HSP90AA1 correlated poorer overall survival was observed. transcription activity AKT1/ERK...
Acute myeloid leukemia (AML) is an aggressive and often deadly malignancy associated with proliferative immature blasts. Here, we identified CD84 as a critical survival regulator in AML. High levels of expression provided advantage to cells, whereas downregulation disrupted their proliferation, clonogenicity engraftment capabilities both human cell lines patient derived xenograft cells. Critically, loss also markedly blocked MLL-AF9 inv(16) AML mouse models, highlighting its pivotal role...
Abstract To investigate reversal effects of pantoprazole (PPZ) on multidrug resistance (MDR) in human gastric adenocarcinoma cells vivo and vitro. Human cell SGC7901 was cultured RPMI‐1640 medium supplemented with 10% fetal bovine serum antibiotics a humidified 5% CO 2 atmosphere at 37°C. Adriamycin (ADR)‐resistant were addition 0.8 µg/ml ADR maintaining MDR phenotype. used to calculate releasing index; CCK‐8 Assay performed evaluate the cytotoxicity anti‐tumor drugs; BCECF‐AM pH‐sensitive...
RING finger protein 187 (RNF187) belongs to domain-containing E3 ligases family, which was recently reported be involved in oncogenesis and development of several cancers. This research aims clarify the role RNF187 colorectal cancer (CRC) development.The expression miR-144-4p were determined by quantitative real-time polymerase chain reaction (qRT-PCR). The levels assessed western blot analysis. Cell Counting Kit-8 (CCK8) assay, clonogenic cell scratch test transwell assay used determine...
Abstract Background Gastric cancer (GC) is one of the most common tumours in East Asia countries and associated with Helicobacter pylori infection. H. utilizes virulence factors, CagA VacA, to up‐regulate pro‐inflammatory cytokines activate NF‐κB signaling. Meanwhile, PIEZO1 upregulation cancer‐associated fibroblast (CAF) enrichment were found GC progression. However, mechanisms its involvement progression have not been fully elucidated. Methods The CAF clinical significance investigated...
Identification of positive regulators T-cell immunity induced during autoimmune diseases is critical for developing novel therapies. The endoplasmic reticulum resident ubiquitin ligase Hrd1 has recently emerged as a regulator dendritic cell antigen presentation, but its role in unknown. Here we show that genetic deletion mice inhibits proliferation, production IL-2, and differentiation Th1 Th17 cells, consequently protects from experimental encephalomyelitis. facilitates proliferation by the...
Recently, several studies have shown that piperlongumine (PL) can selectively kill cancer cells by targeting reactive oxygen species (ROS). However, the potential therapeutic effects and detailed mechanism of PL in gastric are still not clear. In current report, we found significantly suppressed both vitro vivo. obviously increased ROS generation cells. Anti-oxidant glutathione (GSH) N-acetyl-l-cysteine (NAC) abrogate PL-induced cell death proliferation inhibition. GADD45α was induced...
Fis protein is a nucleoid-associated that plays many roles in transcriptional regulation and DNA site-specific recombination. In contrast to the naïve expectation based on stoichiometry, recent single-molecule studies have shown dissociation of from accelerated by increasing concentration protein. Because detailed molecular mechanism facilitated still not clear, this study, we employ computational methods explore binding landscapes Fis:DNA complexes with various stoichiometries. When two...
NCAPG2 is a component of the condensin II complex and contributes to chromosome segregation via microtubule-kinetochore attachment during mitosis. It well known that plays critical role in cell mitosis; however, altered expression its transcriptional regulatory function cancer development remains mostly unknown. Here, for first time we reported was evidently increased non-small lung tissues compared adjacent normal tissues. Clinicopathological data analysis showed overexpression...
Background and Objective The significance of ubiquitin-specific protease 22 (USP22) as a potential marker has been growing in the field oncology. aim this study was to investigate role USP22 association with its targets oral squamous cell carcinoma (OSCC). Methods Immunohistochemistry used determine expression protein 319 OSCC patients comparison 42 healthy controls. clinical correlations prognostic aberrantly expressed evaluated identify novel biomarker OSCC. Results incidence positive...
Gastric carcinoma is one of the most common malignancies worldwide and second frequent cause cancer-related death in China. Protein regulator cytokinesis 1 (PRC1) involved plays key roles microtubule organization eukaryotes. This study was aimed to analyse expression investigate functional role PRC1 gastric tumorigenesis. The evaluated by qRT-PCR, Western blot immunohistochemistry. biological function determined CCK-8 proliferation assays, monolayer colony formation, xenografted nude mice...
CCCTC-binding factor (CTCF) is a DNA-binding protein that interacts with large number of highly divergent target sequences throughout the genome. It implicated in variety functions, including chromatin organization and transcriptional control. The functional role CTCF tumour pathogenesis remains elusive. We showed frequently upregulated subset primary hepatocellular carcinomas (HCCs) as compared non-tumoural liver. Overexpression was associated shorter disease-free survival patients. Short...
In many gastric cancer patients, the disease is diagnosed in an advanced stage and therefore mortality levels are high. Because there a need to identify novel early diagnostic prognostic biomarkers, we tested whether SIRT1 STAT3 good candidates. Towards this, used patient tissues representing different stages of including pre-cancerous lesions, cancer, probed SIRT1, phosphorylated (pSTAT3) using immunohistochemistry. Our results revealed upregulated expression all compared with noncancerous...
Significance BACE1 is a rate-limiting enzyme for amyloid beta polypeptide production, which plays crucial role in Alzheimer’s disease (AD) pathogenesis. However, how this essential protease posttranslationally regulated remains incompletely understood. In the current study, we show that K501 residue on BACE1, ubiquitin modification site, also competitively SUMOylated. We discovered SUMOylation of augments its stability and enzymatic activity, resulting senile plaque formation cognitive...
Abstract Aberrant Notch activation has been implicated in multiple malignancies and the identification of NOTCH receptors related pathways is critical for targeted therapy. In this study, we aim to delineate most prominent dysregulated receptor comprehensively reveal its deregulation gastric cancer (GC). four members, NOTCH3 was found uniformly upregulated associated with poor clinical outcomes GC datasets. siRNA-mediated knockdown demonstrated antitumor effects by suppressing cell...
Abstract Normal Tau promotes the assembly and stabilization of microtubules, thus, maintaining axon transport. In Alzheimer’s disease (AD), aggregation causes it to lose these above-mentioned functions. However, molecular mechanism leading in AD remains ambiguous. Here, we report that USP10, one important deubiquitinases (DUBs), is involved aggregation. We found USP10 upregulated postmortem human APP/PS1 mice brains, but not P301S brains. Moreover, primary neuronal cultures, Aβ 42 induces a...