A5107999131- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Cancer Research and Treatments
- Cancer Cells and Metastasis
- Environmental DNA in Biodiversity Studies
- Microbial Community Ecology and Physiology
- Genetic factors in colorectal cancer
- Gut microbiota and health
- Escherichia coli research studies
- Chemotherapy-induced cardiotoxicity and mitigation
- Helicobacter pylori-related gastroenterology studies
- Colorectal Cancer Screening and Detection
- Kruppel-like factors research
- Cytokine Signaling Pathways and Interactions
- Cancer-related molecular mechanisms research
- Mollusks and Parasites Studies
- RNA modifications and cancer
- Cancer Mechanisms and Therapy
- Identification and Quantification in Food
- Hippo pathway signaling and YAP/TAZ
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Digestive system and related health
- Cancer-related cognitive impairment studies
- Ovarian cancer diagnosis and treatment
National Chung Cheng University
2016-2025
Kaohsiung Medical University
2024
University Health Network
2024
Krembil Research Institute
2024
Hong Kong Green Building Council
2024
Chinese University of Hong Kong
2001-2023
Southern Medical University
2023
Nanfang Hospital
2023
University of Toronto
2016-2020
Trillium Health Centre
2020
Abstract The objective of this study was to identify and characterize a self-renewing subpopulation human ovarian tumor cells (ovarian cancer-initiating cells, OCICs) fully capable serial propagation their original phenotype in animals. Ovarian serous adenocarcinomas were disaggregated subjected growth conditions selective for self-renewing, nonadherent spheroids previously shown derive from tissue stem cells. To affirm the existence OCICs, xenoengraftment as few 100 dissociated spheroid...
Abstract Common sites of breast cancer metastasis include the lung, liver, and bone, these secondary metastatic sites, estrogen receptor α (ERα)–positive often favors bone. Within organs, cells would predictably encounter tissue-specific fibroblasts or their soluble factors, yet our understanding how directly affect cell growth rates survival remains largely unknown. Therefore, we tested hypothesis that mesenchymal isolated from common provide a more favorable microenvironment with respect...
Abstract Testing for DNA methylation has potential in cancer screening. Most previous studies of cervical used a candidate gene approach. The aim our study was to identify novel genes that are methylated cancers and test their clinical applications. We did differential hybridization using CpG island (CGI) microarray containing 8640 CGI tags uncover squamous cell carcinomas (SCC) the uterine cervix. Pooled from tissues normal swabs were comparison. Methylation‐specific polymerase chain...
A recent hypothesis for cancer chemoresistance posits that cytotoxic survival of a subpopulation tumor progenitors drives the propagation recurrent disease, underscoring need new therapeutics target such primitive cells. To discover novel compounds active against drug-resistant ovarian cancer, we identified subset chemoresistant cells fulfilling current definitions cancer-initiating from cell lines and patient tumors using multiple stemness phenotypes, including expression stem markers,...
Abstract Background Altered microbiome composition and aberrant promoter hypermethylation of tumor suppressor genes (TSGs) are two important hallmarks colorectal cancer (CRC). Here we performed concurrent 16S rRNA gene sequencing methyl-CpG binding domain-based capture in 33 tissue biopsies (5 normal colonic mucosa tissues, 4 pairs adenoma adenoma-adjacent 10 CRC CRC-adjacent tissues) to identify significant associations between TSG CRC-associated bacteria, followed by functional validation...
Studies of environmental microbiota typically target only specific groups microorganisms, with most focusing on bacteria through taxonomic classification 16S rRNA gene sequences. For a more holistic understanding microbiome, strategy to characterize the viral, bacterial, and eukaryotic components is necessary. We developed method for metagenomic amplicon-based analysis freshwater samples involving concentration size-based separation eukaryotic, viral fractions. Next-generation sequencing...
Selective detection of biomarkers at low concentrations in blood is crucial for the clinical diagnosis many diseases but remains challenging. In this work, we aimed to develop an ultrasensitive immunoassay that can detect serum with attomolar limit (LOD). We proposed a sandwich-type heterogeneous immunosensor 3 × well array format by integrating resonant waveguide grating (RWG) substrate upconversion nanoparticles (UCNPs). UCNPs were used label target biomarker captured capture antibody...
While promoter hypermethylation is a common molecular alteration of human colorectal cancer that could be detected in the bloodstream, we tested feasibility quantitative detection aberrant DNA methylation multiple genes serum samples patients.The pre-therapeutic 49 patients and 41 age-matched controls with normal colonoscopy were examined. The presence methylated APC (adenomatous polyposis coli), hMLH1 (human MutL homolog 1), HLTF (helicase-like transcription factor) was by...
BACKGROUND Detection of fecal DNA is a promising approach to colorectal cancer screening. However, the sensitivity current tests for polyps low. We evaluated feasibility detecting aberrantly methylated or cyclooxygenase-2 (COX-2) mRNA in feces patients with polyps. METHODS Fecal samples were collected prior colonoscopy from 20 cancer, 30 polyps, and subjects normal colonic examination. Presence hypermethylated 7 tumor-related genes (APC, ATM, hMLH1, sFRP2, HLTF, MGMT, GSTP1) stool was...
Select bacteria, such as Escherichia coli or coliforms, have been widely used sentinels of low water quality; however, there are concerns regarding their predictive accuracy for the protection human and environmental health. To develop improved monitoring systems, a greater understanding bacterial community structure, function, variability across time is required in context different pollution types, agricultural urban contamination. Here, we present year-long survey free-living DNA...
Abstract Plant–animal interactions (PAI) represent major channels of energy transfer through ecosystems, where both positive and antagonistic simultaneously contribute to ecosystem functioning. Monitoring PAI therefore increases the understanding environmental health, integrity, functioning, studying complex accurate, cost‐effective sampling can aid in management detrimental anthropogenic impacts. Environmental DNA (eDNA)‐based monitoring represents an increasingly common, nondestructive...
Abstract Background Gastric cancer (GC) is one of the most common tumours in East Asia countries and associated with Helicobacter pylori infection. H. utilizes virulence factors, CagA VacA, to up‐regulate pro‐inflammatory cytokines activate NF‐κB signaling. Meanwhile, PIEZO1 upregulation cancer‐associated fibroblast (CAF) enrichment were found GC progression. However, mechanisms its involvement progression have not been fully elucidated. Methods The CAF clinical significance investigated...
Promoter hypermethylation is an alternative mechanism of gene silencing in human cancers including gastric cancer. While intestinal metaplasia (IM) generally regarded as a precancerous lesion the stomach, our study examines presence promoter IM patients with and without We examined 31 samples cancer, 36 (21 associated cancer 15 from noncancer patients) 10 normal biopsies. Tissues containing foci were carefully microdissected paraffin-embedded section. Bisulfite-modified DNA was for...
Abstract Trefoil factor family (TFF) domain peptides consist of three members that play a role in intestinal mucosal defence and repair, tumourigenesis. The the TFF gastric carcinogenesis cascade remains poorly defined. This study examined seven cell lines, 50 cancers their adjacent non‐cancer tissues, tissues from 40 patients, order to elucidate chronology expression various stages carcinogenesis. was determined by RT‐PCR, immunohistochemistry, western blot. Aberrant TFF1, TFF2, TFF3...
While there is no reliable serum biomarker for the diagnosis and monitoring of patients with gastric cancer, we tested potential diagnostic prognostic values detecting methylation changes in cancer patients. DNA was extracted from pretherapeutic 60 confirmed adenocarcinoma 22 age-matched noncancer controls. Promoter hypermethylation 10 tumour-related genes (APC, E-cadherin, GSTP1, hMLH1, MGMT, p15, p16, SOCS1, TIMP3 TGF-beta RII) determined by quantitative methylation-specific PCR...
The interleukin-mediated Janus kinase (JAK)/STAT pathway plays a crucial role in carcinogenesis. Recently, increased STAT3 activity was found hepatocellular carcinoma and multiple myeloma which there silencing of SOCS-1 (suppressor cytokine signalling-1) by gene promoter hypermethylation. We investigated the expression level interleukin-6 (IL-6) gastric cancer cell lines. Expression correlated with IL-6 most lines, except for AGS cells absent despite high production. Methylation analysis...
Patients with subarachnoid hemorrhage treated using external ventricular drainage due to obstructive hydrocephalus commonly remain shunt-dependent. Based on identified risk factors for drain (EVD) challenge failure, the authors sought determine likelihood that a patient will require permanent shunt.The reviewed 89 consecutive cases of aneurysmal parameters associated EVD failure and shunt requirement. Significant were combined in discriminant function analysis create index (FRI). Linear...
Rationale: Hyperactivation of Hippo-Yes-associated protein (YAP) signaling pathway governs tumorigenesis gastric cancer (GC). Here we reveal that minichromosome maintenance complex component 6 (MCM6) is a critical transcriptional target YAP in GC. We aim to investigate the function, mechanism action, and clinical implication MCM6 Methods: The downstream targets were screened by RNA sequencing (RNA-seq) microarray, further validated chromatin immunoprecipitation PCR luciferase reporter...