A5065914607- Viral-associated cancers and disorders
- Genetic factors in colorectal cancer
- Cervical Cancer and HPV Research
- Hippo pathway signaling and YAP/TAZ
- Cancer-related molecular mechanisms research
- Cytomegalovirus and herpesvirus research
- RNA modifications and cancer
- Cellular Mechanics and Interactions
- Histiocytic Disorders and Treatments
- Polyomavirus and related diseases
- Protease and Inhibitor Mechanisms
- Lung Cancer Research Studies
- Cell Adhesion Molecules Research
- Cancer, Hypoxia, and Metabolism
- Parvovirus B19 Infection Studies
- Immune Cell Function and Interaction
- Peptidase Inhibition and Analysis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Fibroblast Growth Factor Research
- FOXO transcription factor regulation
- Virus-based gene therapy research
- Neuroendocrine Tumor Research Advances
- DNA and Nucleic Acid Chemistry
- Extracellular vesicles in disease
- Advanced Breast Cancer Therapies
Chinese University of Hong Kong
2015-2025
Prince of Wales Hospital
2023-2025
University of Hong Kong
2011-2022
HKU-Pasteur Research Pole
2017
Hokkaido University
2008
Epstein-Barr virus (EBV) is a herpesvirus associated with nasopharyngeal carcinoma (NPC), gastric (GC), and other malignancies. EBV the first human found to express microRNAs (miRNAs), functions of which remain largely unknown. We report on regulation cellular protein named p53 up-regulated modulator apoptosis (PUMA) by an miRNA known as miR-BART5, abundantly expressed in NPC EBV-GC cells. Modulation PUMA expression miR-BART5 anti–miR-BART5 oligonucleotide was demonstrated EBV-positive In...
Undifferentiated nasopharyngeal carcinomas (NPCs) are commonly present with latent EBV infection. However, events regulating infection at early stages of the disease and role in pathogenesis largely undefined. Genetic alterations leading to activation cyclin D1 signaling premalignant epithelial (NPE) cells have been postulated predispose We previously reported that loss p16, a negative regulator signaling, is frequent feature NPC tumors. Here, we report lesions epithelium overexpress D1....
Abstract The lack of representative nasopharyngeal carcinoma (NPC) models has seriously hampered research on EBV carcinogenesis and preclinical studies in NPC. Here we report the successful growth five NPC patient-derived xenografts (PDXs) from fifty-eight attempts transplantation specimens into NOD/SCID mice. take rates for primary recurrent are 4.9% 17.6%, respectively. Successful establishment a new EBV-positive cell line, NPC43, is achieved directly patient tissues by including...
Abnormal metabolism and uncontrolled angiogenesis are two important characteristics of malignant tumors. The occurrence both events involves many key molecular changes including miRNA. However, EBV encoded miRNAs rarely mentioned as capable regulating tumor angiogenesis. Here, we reported that one the by EBV, EBV-miR-Bart1-5P, can significantly promote nasopharyngeal carcinoma (NPC) cell glycolysis induces in vitro vivo. Mechanistically, EBV-miR-Bart1-5P directly targets α1 catalytic subunit...
Abstract The outbreak caused by the novel coronavirus SARS-CoV-2 has been declared a global health emergency. G-quadruplex structures in genomes have long considered essential for regulating number of biological processes plethora organisms. We analyzed and identified 25 four contiguous GG runs (G2NxG2NyG2NzG2) RNA genome, suggesting putative G-quadruplex-forming sequences (PQSs). Detailed analysis PQSs revealed their locations open reading frames ORF1 ab, spike (S), ORF3a, membrane (M)...
Abstract Interplay between EBV infection and acquired genetic alterations during nasopharyngeal carcinoma (NPC) development remains vague. Here we report a comprehensive genomic analysis of 70 NPCs, combining whole-genome sequencing (WGS) microdissected tumor cells with oncogene expression to reveal multiple aspects cellular-viral co-operation in tumorigenesis. Genomic aberrations along EBV-encoded LMP1 underpin constitutive NF-κB activation 90% NPCs. A similar spectrum somatic viral gene...
Abstract Epstein‐Barr virus (EBV) infection has been postulated to be an early event involved in the pathogenesis of nasopharyngeal carcinomas (NPC). The lack representative premalignant epithelial cell system for EBV hampered research investigation into regulation and involvement NPC pathogenesis. We have compared efficiency cells with different biological properties including immortalized, primary cancerous cells. could achieved all examined variable rate. TGF‐β effectively enhanced both...
Abstract Background Cortidis rhizoma (Huanglian) and its major therapeutic component, berberine, have drawn extensive attention in recent years for their anti-cancer properties. Growth inhibitory effects of berberine on multiple types human cancer cells been reported. Berberine inhibits invasion, induces cell cycle arrest apoptosis cells. The anti-inflammatory property involving inhibition S ignal T ransducer A ctivator ranscription 3 (STAT3) activation, has also documented. Methods In this...
Nasopharyngeal carcinoma (NPC) is etiologically associated with Epstein-Barr virus (EBV) infection. However, the exact role of EBV in NPC pathogenesis remains elusive. Activation signal transducer and activator transcription 3 (STAT3) common human cancers including plays an important progression cancers. Interleukin-6 (IL-6), a major inflammatory cytokine, potent STAT3. In this study, we report that EBV-infected immortalized nasopharyngeal epithelial (NPE) cells often acquire enhanced...
Nasopharyngeal carcinoma (NPC) is strongly associated with Epstein-Barr virus (EBV). We reported that suberoylanilide hydroxamic acid (SAHA) induced EBV lytic cycle in EBV-positive gastric cells and mediated enhanced cell death. However, expression of proteins was thought to exert antiapoptotic effect EBV-infected cells. Here, we examined the vitro vivo effects SAHA on induction NPC investigated cellular consequences. Micromolar concentrations significantly Increased apoptosis proteolytic...
ABSTRACT Accumulating evidence indicates that oncogenic viral protein plays a crucial role in activating aerobic glycolysis during tumorigenesis, but the underlying mechanisms are largely undefined. Epstein-Barr virus (EBV)-encoded latent membrane 1 (LMP1) is transmembrane with potent cell signaling properties and has tumorigenic transformation property. Activation of NF-κB major pathway mediating many downstream LMP1. Here we report activation mTORC1 by LMP1 key modulator for to mediate...
Abstract Nasopharyngeal cancer (NPC), endemic in Southeast Asia, lacks effective diagnostic and therapeutic strategies. Even high-income countries the 5-year survival rate for stage IV NPC is less than 40%. Here we report high somatostatin receptor 2 (SSTR2) expression multiple clinical cohorts comprising 402 primary, locally recurrent metastatic NPCs. We show that SSTR2 induced by Epstein–Barr virus (EBV) latent membrane protein 1 (LMP1) via NF-κB pathway. Using cell-based preclinical...
Abstract Background Gastric cancer (GC) is one of the most common tumours in East Asia countries and associated with Helicobacter pylori infection. H. utilizes virulence factors, CagA VacA, to up‐regulate pro‐inflammatory cytokines activate NF‐κB signaling. Meanwhile, PIEZO1 upregulation cancer‐associated fibroblast (CAF) enrichment were found GC progression. However, mechanisms its involvement progression have not been fully elucidated. Methods The CAF clinical significance investigated...
Abstract The unique virus-cell interaction in Epstein-Barr virus (EBV)-associated malignancies implies targeting the viral latent-lytic switch is a promising therapeutic strategy. However, lack of specific and efficient agents to induce lytic cycle these cancers major challenge facing clinical implementation. We develop synthetic transcriptional activator that specifically activates endogenous BZLF1 efficiently induces reactivation EBV-positive cancer cells. A lipid nanoparticle...
Nasopharyngeal carcinoma (NPC) is common among southern Chinese including the ethnic Cantonese population living in Hong Kong. Epstein-Barr virus (EBV) infection detected all undifferentiated type of NPC this endemic region. Establishment stable and latent EBV premalignant nasopharyngeal epithelial cells an early event development may contribute to its pathogenesis. Immortalized primary represent important tool for investigation tumorigenic potential special cells. However, limited...