A5021070959- Viral-associated cancers and disorders
- Cytomegalovirus and herpesvirus research
- Parvovirus B19 Infection Studies
- Lymphoma Diagnosis and Treatment
- Herpesvirus Infections and Treatments
- Air Quality and Health Impacts
- Histiocytic Disorders and Treatments
- Virus-based gene therapy research
- Polyomavirus and related diseases
- Gut microbiota and health
- Peptidase Inhibition and Analysis
- Toxic Organic Pollutants Impact
- Nuclear Structure and Function
- NF-κB Signaling Pathways
- Eosinophilic Disorders and Syndromes
- Vehicle emissions and performance
- Immune Cell Function and Interaction
- T-cell and Retrovirus Studies
- Heat shock proteins research
- Tuberculosis Research and Epidemiology
- Mitochondrial Function and Pathology
- interferon and immune responses
- Plant Virus Research Studies
- Immune Response and Inflammation
- RNA modifications and cancer
Research Center for Eco-Environmental Sciences
2025
University of Chinese Academy of Sciences
2025
State Key Joint Laboratory of Environment Simulation and Pollution Control
2025
Chinese Academy of Sciences
2025
Duke University
2025
Inner Mongolia Agricultural University
2025
National Taiwan University
2013-2024
Second Hospital of Hebei Medical University
2023-2024
Dalian Medical University
2017-2024
First Affiliated Hospital of Dalian Medical University
2017-2024
The cellular endosomal sorting complex required for transport (ESCRT) machinery participates in membrane scission and cytoplasmic budding of many RNA viruses. Here, we found that expression dominant negative ESCRT proteins caused a blockade Epstein-Barr virus (EBV) release retention viral BFRF1 at the nuclear envelope. adaptor protein Alix was redistributed partially colocalized with rim replicating cells. Following transient transfection, associated proteins, reorganized induced perinuclear...
The BGLF4 protein kinase of Epstein-Barr virus (EBV) is a member the conserved family herpesvirus kinases which, to some extent, have function similar that cellular cyclin-dependent in regulating multiple and viral substrates. In yeast two-hybrid screening assay, splicing variant interferon (IFN) regulatory factor 3 (IRF3) was found interact with protein. This interaction defined further by coimmunoprecipitation transfected cells glutathione S-transferase (GST) pull-down vitro. Using...
DNA viruses adopt various strategies to modulate the cellular environment for efficient genome replication and virion production. Previously, we demonstrated that BGLF4 kinase of Epstein-Barr virus (EBV) induces premature chromosome condensation through activation condensin topoisomerase IIalpha (C. P. Lee, J. Y. Chen, T. Wang, K. Kimura, A. Takemoto, C. Lu, M. R. Virol. 81:5166-5180, 2007). In this study, show interacts with lamin A/C phosphorylates A protein in vitro. Using a green...
Epstein-Barr virus (EBV) expresses few viral proteins in nasopharyngeal carcinoma (NPC) but high levels of BamHI-A rightward transcripts (BARTs), which include long noncoding RNAs (lncRNAs) and BART microRNAs (miRNAs). It is hypothesized that the mechanism for regulation BARTs may relate to EBV pathogenesis NPC. We showed nuclear factor-κB (NF-κB) activates promoters modulates expression EBV-infected NPC cells introduction mutations into putative NF-κB binding sites abolished activation by...
Abstract The unique virus-cell interaction in Epstein-Barr virus (EBV)-associated malignancies implies targeting the viral latent-lytic switch is a promising therapeutic strategy. However, lack of specific and efficient agents to induce lytic cycle these cancers major challenge facing clinical implementation. We develop synthetic transcriptional activator that specifically activates endogenous BZLF1 efficiently induces reactivation EBV-positive cancer cells. A lipid nanoparticle...
EBNA-1 is essential for replication of the latent episomal form Epstein-Barr virus genome and involved in regulation viral latency promoters. activity mediated through direct DNA binding. The binding dimerization functions have previously been located to a carboxy-terminal domain, amino acids (aa) 459 607. To identify define subdomains these two functions, we created an extensive series deletions point mutations (aa 408 641) background. ability mutants heterodimerize with wild-type...
Host immune system is designed (or evolved) to fight against different pathogens. Many viruses infect the cells for propagation of new progenies, thus infection may modulate host homeostasis. It has been more than 45 years since discovery Epstein–Barr virus (EBV) from a Burkitt's lymphoma derived cell line. The ability EBV transform primary B in vitro leads suggestion its oncogenic potential. However, except clear understanding role post-transplantation lymphoproliferative disease, it...
Epstein-Barr virus (EBV) induces an uncoordinated S-phase-like cellular environment coupled with multiple prophase-like events in cells replicating the virus. The EBV encoded Ser/Thr kinase BGLF4 has been shown to induce premature chromosome condensation through activation of condensin and topoisomerase II reorganization nuclear lamina facilitate egress nucleocapsids a pathway mimicking Cdk1. However, observation that RB is hyperphosphorylated presence raised possibility may have Cdk2-like...
ABSTRACT The Epstein-Barr virus (EBV) open reading frame BGLF4 was identified as a potential Ser/Thr protein kinase gene through the recognition of amino acid sequence motifs characteristic conserved regions within catalytic domains kinases. In order to investigate this activity, expressed in Escherichia coli and purified used generate specific antiserum. Recombinant vaccinia vTF7-3, which expresses T7 RNA polymerase, infect 293 293T cells after transient transfection with plasmid containing...
Previous studies of Epstein-Barr virus (EBV) replication focused mainly on the viral and cellular factors involved in compartment assembly controlling cell cycle. However, little is known about how EBV reorganizes nuclear architecture chromatin territories. In EBV-positive nasopharyngeal carcinoma NA cells or Akata cells, we noticed that becomes highly condensed upon reactivation. searching for possible mechanisms involved, found transient expression BGLF4 kinase induces unscheduled...
ABSTRACT A conserved family of herpesvirus protein kinases plays a crucial role in DNA replication and virion production. However, despite the fact that these are potential therapeutic targets, no systematic studies have been performed to identify their substrates. We generated an Epstein-Barr virus (EBV) array evaluate targets EBV kinase BGLF4. Multiple proteins involved lytic assembly were identified as previously unrecognized substrates for BGLF4, illustrating broad played by this kinase....
Abstract Background Previous studies on the effects of aging in human and mouse mesenchymal stem cells suggest that a decline number differentiation potential may contribute to aging-related diseases. In this report, we used stromal isolated from adipose tissue (ADSCs) young (8-10 weeks), adult (5 months), old (21 months) mice test hypothesis mechanical loading modifies changes self-renewal osteogenic adipogenic these cells. Results We show significantly reduced proliferation increased...
The cellular endosomal sorting complex required for transport (ESCRT) was recently found to mediate important morphogenesis processes at the nuclear envelope (NE). We previously showed that Epstein-Barr virus (EBV) BFRF1 protein recruits ESCRT-associated Alix modulate NE structure and promote EBV egress. Here, we uncover new factors mechanisms involved in this process. BFRF1-induced vesicles are similar those observed following reactivation. is ubiquitinated, elimination of possible...
Abstract Question Quantifying the duration and drivers of seedling persistence is critical for understanding dynamics species co‐existence in plant communities. In this study, we incorporated data from multiple censuses to characterize patterns a tropical karst forest. Specifically, evaluated effects density dependence, habitat heterogeneity recruitment timing on persistence. Location A forest Taiwan. Methods Using 144 plots censused every 3 mo 2007 2012, examined times 6399 seedlings 36...
Abstract Background Atopic dermatitis (AD) often arises in infancy, and gut microbial dysbiosis is associated with the development of AD. However, less known about specific changes early‐life microbiome AD severity. This study aims to reveal composition function profiles severity infants. Methods Sixty‐two infants (mean [SD] age, 4.7[1.9] months) different severities were enrolled divided into three groups (mild, moderate severe) according Scoring Dermatitis (SCORAD) index. The analysed by...
BGLF4 is the only serine/threonine protein kinase identified in Epstein-Barr virus (EBV); it known to phosphorylate viral DNA polymerase processivity factor, EA-D (BMRF1), EBNA-LP, EBNA-2, cellular EF-1delta and nucleoside analogue ganciclovir. However, expression biological functions of have not yet been clearly demonstrated EBV-infected cells. To reveal authentic within viral-replicating cells, a panel specific monoclonal antibodies was generated characterized. The major immunogenic...
ABSTRACT Epstein-Barr virus (EBV) BGLF4 is a member of the conserved herpesvirus kinases that regulate multiple cellular and viral substrates play an important role in lytic cycles. has been found to phosphorylate several transcription factors, modulate their activities, downstream events. In this study, we identify NF-κB coactivator, UXT, as substrate BGLF4. downregulates not only transactivation reporter assays response tumor necrosis factor alpha (TNF-α) poly(I·C) stimulation, but also...
ABSTRACT Epstein-Barr virus (EBV) BKRF3 shares sequence homology with members of the uracil-N-glycosylase (UNG) protein family and has DNA glycosylase activity. Here, we explored how participates in replication complex contributes to viral replication. Exogenously expressed Flag-BKRF3 was distributed mostly cytoplasm, whereas translocated into nucleus colocalized EBV polymerase BALF5 compartment during lytic The expression level increased gradually replication, coupled a decrease cellular...