A5079918383- Sarcoma Diagnosis and Treatment
- Reproductive Biology and Fertility
- Influenza Virus Research Studies
- Ovarian cancer diagnosis and treatment
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- Uterine Myomas and Treatments
- Ultrasound and Hyperthermia Applications
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Microtubule and mitosis dynamics
- Viral-associated cancers and disorders
- Advanced optical system design
- Genetics, Aging, and Longevity in Model Organisms
- Ophthalmology and Visual Impairment Studies
- Vascular Tumors and Angiosarcomas
- Liver Disease Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- Cytomegalovirus and herpesvirus research
- PARP inhibition in cancer therapy
University of Hong Kong
2014-2024
Queen Mary Hospital
2024
HKU-Pasteur Research Pole
2011-2021
State Key Laboratory of Respiratory Disease
2015
Public health risks associated to infection by human coronaviruses remain considerable and vaccination is a key option for preventing the resurgence of severe acute respiratory syndrome coronavirus (SARS-CoV). We have previously reported that antibodies elicited SARS-CoV vaccine candidate based on recombinant, full-length Spike-protein trimers, trigger immune cell lines. These observations prompted us investigate molecular mechanisms responses antibody-mediated in macrophages. used primary...
ABSTRACT A novel avian-origin influenza A/H7N9 virus emerged in 2013 to cause more than 130 cases of zoonotic human disease, with an overall case fatality rate around 30% detected. It has been shown that E-to-K amino acid change at residue 627 polymerase basic protein 2 (PB2) occurred frequently the H7N9 isolates obtained from humans but not viruses isolated poultry. Although this mutation reported confer increased mammalian pathogenicity other avian subtypes, it experimentally investigated...
Abstract The unique virus-cell interaction in Epstein-Barr virus (EBV)-associated malignancies implies targeting the viral latent-lytic switch is a promising therapeutic strategy. However, lack of specific and efficient agents to induce lytic cycle these cancers major challenge facing clinical implementation. We develop synthetic transcriptional activator that specifically activates endogenous BZLF1 efficiently induces reactivation EBV-positive cancer cells. A lipid nanoparticle...
A novel avian-origin influenza A/H7N9 virus infecting humans was first identified in March 2013 and, as of 30 May 2013, has caused 132 human infections leading to 33 deaths. Phylogenetic studies suggest that this is a reassortant, with the surface hemagglutinin (HA) and neuraminidase (NA) genes being derived from duck wild-bird viruses, respectively, while six "internal gene segments" were poultry H9N2 viruses. Here we determine pathogenicity A/Shanghai/2/2013 (Sh2/H7N9) healthy adult mice...
Influenza A subtype H9N2 is widespread and prevalent in poultry. It has repeatedly transmitted zoonotically to cause mild influenza-like illness humans regarded as a potential pandemic candidate. In additon, the six internal genes of H7N9 H10N8 viruses which caused infection human China well some highly pathogenic H5N1 strains are origined from H9N2. Previous studies have shown that mammalian adaptation PB2-Q591K contributes pathogenicity viruses. However, role mutation still not...
Background The second wave of avian influenza H7N9 virus outbreak in humans spread to the Guangdong province China by August 2013 and this is now endemic poultry region. Methods Five patients with infection admitted our hospital during February 2014 were intensively investigated. Viral load respiratory tract was determined quantitative polymerase chain reaction (Q-PCR) cytokine levels measured bead-based flow cytometery. Results Four survived one died. different clinical specimens correlated...
Avian influenza A (H7N9) viruses emerged in China 2013 and caused zoonotic disease associated with a case-fatality ratio of over 30%. Transcriptional profiles peripheral blood reflect host responses can help to elucidate pathogenesis.We correlated serial transcriptomic patients avian virus infection determined the biological significances from analysis.We found that specific gene expression were strongly Pao 2/Fio 2 viral load lower respiratory tract. Cell cycle leukocyte-related immunity...
A novel avian-origin influenza (H7N9) virus emerged in China 2013 and has caused zoonotic disease over 1123 persons with an overall mortality around 30%. Amino acid changes at the residues 591, 627 701 of polymerase basic protein 2 (PB2) have been found frequently human H7N9 isolates but not viruses isolated from avian species. We recently identified a cluster ducks which circulated prior to first recognition 2013. These duck genetic background distinct lineage. that introduction PB2...
Female genital melanomas are rare. At diagnosis, most affected patients have advanced disease. Surgery remains the primary treatment, and adjuvant therapy is largely ineffective. Recently, immune checkpoints mitogen-activated protein kinase pathway been explored as treatment targets. However, evaluation of these biomarkers in limited. We evaluated clinicopathological features 20 vulvar, 32 vaginal, three cervical assessed programmed cell death ligand 1 (PD-L1) expression, CD8...
The Arias-Stella reaction (ASR) can mimic endometrial clear cell carcinoma (ECCC) in small biopsies, especially when drug or pregnancy history is unknown. A panel of immunohistochemical markers comprising napsin A, hepatocyte nuclear factor-1-beta (HNF-1β), estrogen and progesterone receptors (ER, PR) has been found useful confirming a diagnosis ECCC. However, the detailed characterization how expression this combination ECCC mimics ASR yet to be thoroughly evaluated.The frequency extent...
Antibody-dependent enhancement (ADE) of viral replication has been documented for viruses, such as dengue virus, Ross river other alpha and flaviviruses, HIV also influenza virus. ADE occurs when non-neutralised virus-antibody complexes find alternative receptors routes entry into the cell via Fc-receptor pathway. demonstrated predominantly in macrophages or bearing cells although types have occasionally implicated (1,2). Thus, viruses may to lacking usual virus receptor. Alternatively,...
To evaluate whether a selected group of 9 children with history congenital esotropia is capable producing vergence eye responses to fusional disparity stimuli.Nine and 5 age-matched normal binocular vision were examined. Using full-field target, base out 3 prism diopters placed in front both eyes recorded.In five patients, the initial response was saccade generated by dominant eye, followed disconjugate movement one or eyes. In two patients long standing uncorrected strabismus, almost purely...
Abstract Interferon stimulated gene 15 (ISG15) is a ubiquitin like modifier frequently induced during virus infections and involved in versatile host defense mechanisms. Not surprisingly, many viruses including SARS-CoV-2 have evolved de-ISGylating activities to antagonize its effect. In this study we compared ISG15-driven macrophage responses upon infection by influenza, Zika viruses. ISG15 modifying enzymes were upregulated human macrophages after with all three While influenza cellular...
Abstract Purpose: Uterine leiomyosarcoma (LMS) is an aggressive sarcoma and a subset of which exhibits DNA repair defects. Polo-like kinase 4 (PLK4) precisely modulates mitosis, its inhibition causes chromosome missegregation increased damage. We hypothesize that PLK4 effective LMS treatment. Experimental Design: Genomic profiling clinical uterine samples was performed, homologous recombination (HR) deficiency scores were calculated. A inhibitor (CFI-400945) with without ataxia...
<div>AbstractPurpose:<p>Uterine leiomyosarcoma (LMS) is an aggressive sarcoma and a subset of which exhibits DNA repair defects. Polo-like kinase 4 (PLK4) precisely modulates mitosis, its inhibition causes chromosome missegregation increased damage. We hypothesize that PLK4 effective LMS treatment.</p>Experimental Design:<p>Genomic profiling clinical uterine samples was performed, homologous recombination (HR) deficiency scores were calculated. A inhibitor...
<div>AbstractPurpose:<p>Uterine leiomyosarcoma (LMS) is an aggressive sarcoma and a subset of which exhibits DNA repair defects. Polo-like kinase 4 (PLK4) precisely modulates mitosis, its inhibition causes chromosome missegregation increased damage. We hypothesize that PLK4 effective LMS treatment.</p>Experimental Design:<p>Genomic profiling clinical uterine samples was performed, homologous recombination (HR) deficiency scores were calculated. A inhibitor...
<p>Supplementary Data S1. Small variants, copy number alterations, structural and fusion transcripts of DNA repair genes.</p>
<p>Supplementary Figure S5. Oncoplot of key genes responsible for DNA repair among sarcoma cell lines.</p>
<p>Supplementary Figure S6. Genomic alterations involving key genes responsible for DNA repair in uterine leiomyosarcoma.</p>
<p>Supplementary Figure S2. DNA content and centrosome number quantification in myogenic sarcoma cell lines after CFI-400945 treatment.</p>
<p>Supplementary Figure S4. Autopsy findings on two mice from different 5 mg/kg CFI-400945 treatment groups that were withdrawn the experiments (hematoxylin and eosin staining).</p>
<p>Supplementary Figure S4. Autopsy findings on two mice from different 5 mg/kg CFI-400945 treatment groups that were withdrawn the experiments (hematoxylin and eosin staining).</p>