A5101806776- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- Ubiquitin and proteasome pathways
- DNA Repair Mechanisms
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- DNA and Nucleic Acid Chemistry
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Cancer therapeutics and mechanisms
- Kruppel-like factors research
- Cell death mechanisms and regulation
- Black Holes and Theoretical Physics
- Telomeres, Telomerase, and Senescence
- Estrogen and related hormone effects
- Cancer-related gene regulation
- Cerebrovascular and Carotid Artery Diseases
- Metabolism, Diabetes, and Cancer
- Rheumatoid Arthritis Research and Therapies
- Plant nutrient uptake and metabolism
Waseda University
2023
University of Tsukuba
2011-2021
Life University
2016
Tohoku University
2008-2014
National Taiwan University
2007-2008
National Health Research Institutes
2008
The University of Tokyo
1996-2008
Tokyo University of Science
2007
Japan Science and Technology Agency
2006-2007
RIKEN Advanced Science Institute
2004-2006
13S condensin is a multisubunit protein complex essential for mitotic chromosome condensation in Xenopus egg extracts. Purified introduces positive supercoils into DNA the presence of topoisomerase I and adenosine triphosphate vitro. The supercoiling activity 13Scondensin was regulated by mitosis-specific phosphorylation. Immunodepletion, vitro phosphorylation, peptide-mapping experiments indicated that Cdc2 likely to be kinase phosphorylates activates condensin. Multiple phosphorylation...
We analyze the moduli space of low-energy limit 3-dimensional Maxwell-Chern-Simons theories described by circular quiver diagrams, as for 4-dimensional elliptic models. define using D3-NS5-(k,1)5-brane systems with an arbitrary number fivebranes. The supersymmetry is expected to be enhanced in limit. show that Higgs branch, which all bifundamental scalar fields develop vacuum expectation values, abelian orbifold ℂ4. confirm same geometry obtained M-theory dual brane system. also consider...
Estrogen is a growth factor that stimulates cell proliferation. The effects of estrogen are mediated through the receptors, ERalpha and ERbeta, which function as ligand-induced transcription factors belong to nuclear receptor superfamily. On other hand, TGF-beta acts inhibitor, its signaling transduced by Smads. Although number studies have been made on cross-talk between estrogen/ERalpha TGF-beta/Smad signaling, whose molecular mechanisms remain be determined. Here, we show inhibits...
The 5S ribonucleoprotein particle (RNP) complex, consisting of RPL11, RPL5, and rRNA, is implicated in p53 regulation under ribotoxic stress. Here, we show that the RNP contributes to activation promotes cellular senescence response oncogenic or replicative Oncogenic stress accelerates rRNA transcription delays processing, resulting RPL11 RPL5 accumulation ribosome-free fraction, where they bind MDM2. Experimental upregulation downregulation mimicking nucleolus stress, respectively, also...
13S condensin is a five-subunit protein complex that plays central role in mitotic chromosome condensation. The was originally identified and purified fromXenopus egg extracts shown to have an ATP-dependent positive supercoiling activity vitro. We report here the characterization of human from HeLa cell nuclear extracts. has exactly same composition as its Xenopuscounterpart, being composed two structural maintenance chromosomes (human chromosome-associated polypeptide (hCAP)-C hCAP-E)...
Condensin is a multisubunit protein complex that reconfigures DNA structure in an ATP-dependent manner vitro and plays central role mitotic chromosome condensation Xenopus egg cell-free extracts. The 13S condensin (13SC) composed of two subcomplexes: 8S core subcomplex (8SC) consisting structural maintenance chromosomes (SMC) subunits (XCAP-C -E) 11S regulatory (11SR) containing three non-SMC (XCAP-D2, -G, -H). We report here the biochemical functional dissection this machinery. Although...
A family of serine/threonine kinase Aurora constitutes a key regulator in the orchestration mitotic events. The human paralogues Aurora-A, Aurora-B, and Aurora-C have highly conserved catalytic domain. Extensive studies on role Aurora-A Aurora-B revealed distinct localizations functions regulating processes, whereas little is known about Aurora-C. present study shows that chromosomal passenger protein forms complexes with inner centromere (INCENP), which are proteins. We show INCENP binds...
Clinical evidence suggests that antiestrogens inhibit the development of androgen-insensitive prostate cancer. Here, we show estrogen receptor β (ERβ) mediates inhibition by antiestrogen ICI 182,780 (ICI) and its enhancement estrogen. ERβ associated with gene promoters through tumor-suppressing transcription factor KLF5 (Krüppel-like zinc finger 5). treatment increased recruitment coactivator CBP [CREB (adenosine 3',5'-monophosphate response element-binding protein)-binding protein] to...
Responding to various stimuli is indispensable for the maintenance of homeostasis. The downregulation ribosomal RNA (rRNA) transcription one mechanisms involved in response by cellular processes, such as cell cycle arrest and apoptosis. Cell differentiation caused intra- extracellular associated with rRNA well reduced growth. during considered contribute However, can induce processes; therefore, it may positively regulate differentiation. To test this possibility, we specifically...
Previous studies of Epstein-Barr virus (EBV) replication focused mainly on the viral and cellular factors involved in compartment assembly controlling cell cycle. However, little is known about how EBV reorganizes nuclear architecture chromatin territories. In EBV-positive nasopharyngeal carcinoma NA cells or Akata cells, we noticed that becomes highly condensed upon reactivation. searching for possible mechanisms involved, found transient expression BGLF4 kinase induces unscheduled...
During mitosis, chromosome condensation takes place, which entails the conversion of interphase chromatin into compacted mitotic chromosomes. Condensin I is a five-subunit protein complex that plays central role in this process. targeted to chromosomes mitosis-specific manner, regulated by phosphorylation kinases. Phosphorylation histone H3 at serine 10 (Ser10) occurs during mitosis and its physiological longstanding question. We examined function Aurora B, kinase phosphorylates Ser10,...
Recent studies have shed light on the ligand-dependent transactivation mechanisms of nuclear receptors (NRs). When ligand dose is reduced, transcriptional activity NRs should be downregulated. Here we show that a ubiquitin-proteasome pathway plays key role in turning off transcription mediated by estrogen receptor beta (ERbeta). ERbeta shows estrogen-dependent proteolysis, and its degradation regulated two regions receptor. The N-terminal 37-amino acid-region necessary for recruitment...
Inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease, is a chronic intestinal inflammatory condition initiated by integrins-mediated leukocyte adhesion to the activated colonic microvascular endothelium. Calreticulin (CRT), calcium-binding chaperone, known as partner in activation of integrin α subunits (ITGAs). The relationship between their interaction pathogenesis IBD largely unknown. Here we show that small molecule, orally active ER-464195-01, inhibits CRT...
Condensin, one of the most abundant components mitotic chromosomes, is a conserved protein complex composed two structural maintenance chromosomes (SMC) subunits (SMC2- and SMC4-type) three non-SMC subunits, it plays an essential role in chromosome condensation. Purified condensin reconfigures DNA structure using energy provided by ATP hydrolysis. To know regulation somatic cells, expression level, subcellular localization, phosphorylation status human were examined during cell cycle. The...
The present study investigates the regulatory effects of glycosaminoglycans such as heparin and heparan sulfate on T cell proliferation induced by thymic stromal monolayer or its derived growth factor (TCGF). A clone (MRL104.8a) supported Ag-specific, IL-2-dependent Th (9-16) in absence Ag IL-2 producing a unique TCGF designated stroma-derived (TSTGF). addition to cultures which 9-16 cells was otherwise stimulated MRL104.8a semipurified sample TSTGF resulted dose-dependent inhibition...
Tetramerization of p53 is crucial to exert its biological activity, and nucleolar disruption sufficient activate p53. We previously demonstrated that stress induces translocation the protein MYBBP1A from nucleolus nucleoplasm enhances activity. However, whether how regulates tetramerization in response remain unclear. In this study, we tetramerization, followed by acetylation under stress. found has two regions directly bind lysine residues C-terminal regulatory domain. formed a...