A5045067702- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Circadian rhythm and melatonin
- Mitochondrial Function and Pathology
- Trace Elements in Health
- RNA modifications and cancer
- Chemical Synthesis and Analysis
- Genetics, Aging, and Longevity in Model Organisms
- Neurobiology and Insect Physiology Research
- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Receptor Mechanisms and Signaling
- Heat shock proteins research
- Photoreceptor and optogenetics research
- RNA and protein synthesis mechanisms
- FOXO transcription factor regulation
- Mast cells and histamine
- Cell Adhesion Molecules Research
- Pancreatic function and diabetes
- Light effects on plants
- Peptidase Inhibition and Analysis
- RNA Research and Splicing
- Machine Learning in Bioinformatics
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Renin-Angiotensin System Studies
University of Tsukuba
2014-2024
Life Science Center for Survival Dynamics, Tsukuba Advanced Research Alliance (TARA)
2016-2023
National Institute of Advanced Industrial Science and Technology
1997-2003
Institute of Applied Biochemistry
1993-2002
The <i>period</i> (<i>per</i>) gene, controlling circadian rhythms in <i>Drosophila</i>, is expressed throughout the body a manner. A homolog of <i>Drosophila per</i> was isolated from rat and designated as<i>rPer2</i>. rPER2 protein showed 39 95% amino acid identity with mPER1 mPER2 (mouse homologs <i>per</i>) proteins, respectively. robust fluctuation <i>rPer2</i> mRNA expression discovered not only suprachiasmatic nucleus (SCN) hypothalamus but also other tissues including eye, brain,...
ABSTRACT Ribosomal RNAs (rRNAs) act as scaffolds and ribozymes in ribosomes, these functions are modulated by post-transcriptional modifications. However, the biological role of base methylation, a well-conserved modification rRNA, is poorly understood. Here, we demonstrate that nucleolar factor, nucleomethylin (NML; also known RRP8), required for N1-methyladenosine (m1A) 28S rRNAs human mouse cells. NML contributes to 60S ribosomal subunit formation. Intriguingly, depletion increases...
Clinical evidence suggests that antiestrogens inhibit the development of androgen-insensitive prostate cancer. Here, we show estrogen receptor β (ERβ) mediates inhibition by antiestrogen ICI 182,780 (ICI) and its enhancement estrogen. ERβ associated with gene promoters through tumor-suppressing transcription factor KLF5 (Krüppel-like zinc finger 5). treatment increased recruitment coactivator CBP [CREB (adenosine 3',5'-monophosphate response element-binding protein)-binding protein] to...
Cox17p is essential for the assembly of functional cytochrome c oxidase (CCO) and delivery copper ions to mitochondrion insertion into enzyme in yeast. Although this small protein has already been cloned or purified from humans, mice, pigs, function mammalian system not yet elucidated. In vitro biochemical data indicate that binds sequence -KPCCAC-. mouse embryos homozygous COX17 disruption die between embryonic days E8.5 E10, they develop normally until E6.5. This phenotype strikingly...
PRMT8 directly hydrolyzes phosphatidylcholine, which is important for brain functions.
Immunological memory is a hallmark of the adaptive immune system. Although natural killer (NK) cells are innate important for immediate host defence, they can differentiate into NK cells. The molecular mechanisms controlling this differentiation yet to be fully elucidated. Here we identify scaffold protein Themis2 as critical regulator cell and function. Themis2-deficient expressing Ly49H, an activating receptor mouse cytomegalovirus (MCMV) antigen m157, show enhanced augment protection...
Apelin peptide is an endogenous ligand of APJ (a putative receptor protein related to the angiotensin II type 1 receptor), which a member G protein-coupled superfamily with seven transmembrane domains. Recent findings have suggested that apelin-APJ system plays potential role in cardiac contraction and cardioprotection. In present study, we show disrupted doxorubicin (Dox)-induced cardiotoxicity. We found downregulation apelin mRNA expression C57Bl/6J mouse hearts on days 5 after Dox...
Heart failure and chronic kidney disease are major causes of morbidity mortality internationally. Although these dysfunctions common frequently coexist, the factors involved in their relationship cardiorenal regulation still largely unknown, mainly due to a lack detailed molecular targets. Here, we found increased plasma histamine preclinical mouse model severe cardiac dysfunction, that had been cotreated with angiotensin II (Ang II), nephrectomy, salt (ANS). The ANS mice exhibited impaired...
ω-NG-monomethylarginine (MMA) and asymmetric ω-NG, ω-NG-dimethylarginine (ADMA), are endogenous competitive inhibitors for three isoforms of nitric oxide synthase (NOS). Although free methylarginines thought to be liberated through the intracellular proteolysis proteins methylated by protein arginine methyltransferases (PRMTs), degradation pathways arginine-methylated involved in biosynthesis have yet determined. In this study, with cultured cells was analyzed as follows: first, we...
RNAs are post-transcriptionally modified in all kingdoms of life. Of these modifications, base methylations highly conserved eukaryote ribosomal RNA (rRNA). Recently, rRNA processing protein 8 (Rrp8) and nucleomethylin (NML) were identified as factors N1-methyladenosine (m1A) modification yeast 25 S mammalian 28 rRNA, respectively. However, m1A is still poorly understood Caenorhabditis elegans (C. elegans). Here, using the liquid chromatography/tandem mass spectrometry analysis...
Protein arginine methyltransferase 1 (PRMT-1) catalyzes asymmetric dimethylation on cellular proteins and modulates various aspects of biological processes, such as signal transduction, DNA repair, transcriptional regulation. We have previously reported that the null mutant prmt-1 in Caenorhabditis elegans exhibits a slightly shortened life span, but physiological significance PRMT-1 remains largely unclear. Here we explored role mitochondrial function hinted by two-dimensional Western...
Previous works have reported the isolation of a novel polypeptide from porcine heart. Structural analysis has shown that it is mammalian homologue Cox17p, believed essential for assembly functional cytochrome c oxidase and delivery copper ions to mitochondrion insertion into enzyme in yeast. Although human, mouse homologs this small protein already been cloned or purified, function Cox17p system not yet elucidated. To investigate physiological mammals, we performed Northern blot using probes...
Protein arginine methyltransferases (PRMTs) catalyze the transfer of a methyl group from S-adenosylmethionine to residues and are classified into two types: type I producing asymmetric dimethylarginine (ADMA) II symmetric (SDMA). PRMTs have been shown regulate many cellular processes, including signal transduction, transcriptional regulation RNA processing. Since loss-of-function mutation PRMT1 PRMT5, each which is predominant II, respectively, causes embryonic lethality in mice, their...
Abstract: It has been suggested that murine neuroblastoma C1300 cells express endogenous neurokinin NK 2 receptors with features differ from those of characterized in other systems. In this study, we have further the receptor types present cell line. RNA blots showed mRNAs and 3 receptors, but not 1 were expressed cells. The increase cytosolic calcium concentration ([Ca 2+ ] i ) induced by 0.33 µ M A was completely inhibited SR 48968, an antagonist, whereas partial response to B unaffected,...