A5051273597- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Breast Cancer Treatment Studies
- RNA and protein synthesis mechanisms
- Advanced Breast Cancer Therapies
- Biological and pharmacological studies of plants
- Advanced Nanomaterials in Catalysis
- Biosensors and Analytical Detection
- Cancer Genomics and Diagnostics
- Breast Lesions and Carcinomas
- Protein Interaction Studies and Fluorescence Analysis
- Nanocluster Synthesis and Applications
- HER2/EGFR in Cancer Research
- Bacteriophages and microbial interactions
- Carbon and Quantum Dots Applications
- Viral gastroenteritis research and epidemiology
- Photosynthetic Processes and Mechanisms
- Cancer Treatment and Pharmacology
- Proteins in Food Systems
- Molecular Sensors and Ion Detection
- Drug Solubulity and Delivery Systems
- MicroRNA in disease regulation
- Phytochemistry and biological activity of medicinal plants
- Ginseng Biological Effects and Applications
Chinese Academy of Medical Sciences & Peking Union Medical College
2023-2025
Jilin University
2016-2024
Soochow University
2024
City University of Hong Kong
2019-2024
State Key Laboratory of Marine Pollution
2024
Cancer Hospital of Chinese Academy of Medical Sciences
2024
Kowloon Hospital
2019
Zhengzhou University
2017-2018
Fudan University Shanghai Cancer Center
2018
Abstract The outbreak caused by the novel coronavirus SARS-CoV-2 has been declared a global health emergency. G-quadruplex structures in genomes have long considered essential for regulating number of biological processes plethora organisms. We analyzed and identified 25 four contiguous GG runs (G2NxG2NyG2NzG2) RNA genome, suggesting putative G-quadruplex-forming sequences (PQSs). Detailed analysis PQSs revealed their locations open reading frames ORF1 ab, spike (S), ORF3a, membrane (M)...
Abstract Rational design of aptamers to incorporate unnatural nucleotides and special chemical moieties can expand their functional complexity diversity. Spiegelmer (L-RNA aptamer) is a unique class aptamer that composed L-RNA nucleotides, so far there are limited candidates applications being reported. Moreover, the target binding properties current require significant improvement. Here, using L-Apt.4-1c as an example, we develop simple robust strategy generate first circular aptamer,...
RNA encodes sequence- and structure-dependent interactions to modulate the assembly properties of biomolecular condensates. G-quadruplexes (rG4s) formed by guanine-rich sequences can trigger formation liquid- or solid-like condensates that are involved in many aberrant phase transitions. However, exactly how rG4 motifs different transitions impart distinct material is unclear. Here, using oligonucleotides cationic peptides as model systems, we show RNA-peptide exhibit tunability over a wide...
G-quadruplexes (G4) are special nucleic acid structures with diverse conformational polymorphisms. Selective targeting of G-quadruplex conformations and regulating their biological functions provide promising therapeutic intervention. Despite the large repertoire G4-binding tools, only a limited number them can specifically target particular G4 conformation. Here, we introduce novel method, G4-SELEX-Seq report development first L-RNA aptamer, L-Apt12-6, high binding selectivity to parallel...
Poorly soluble drugs have low bioavailability after oral administration, thereby hindering effective drug delivery. A novel drug-delivery system of docetaxel (DTX)-based stearic acid (SA)-modified Bletilla striata polysaccharides (BSPs) copolymers was successfully developed. Particle size, zeta potential, encapsulation efficiency (EE), and loading capacity (LC) were determined. The DTX release percentage in vitro determined using high performance liquid chromatography (HPLC). hemolysis...
We developed self-microemulsifying drug-delivery systems (SMEDDS), including bile salts, to improve the oral bioavailability of pueraria flavones (PFs). The physical properties SMEDDS using Cremophor RH 40, and salts as mixed surfactants at weight ratios 10:0⁻0:10 were determined. particle sizes PFs-SMEDDSNR containing sodium taurocholate (NaTC) PFs-SMEDDSR 40 measured upon dilution with deionized water other aqueous media. Dilution volume presented no remarkable effects on size, whereas...
A label-free biosensor was developed for highly sensitive and selective determination of Exo III based on poly(T) molecular beacon-templated CuNPs.
Guanine quadruplex (G4) structure is a four-stranded nucleic acid secondary motif with unique chemical properties and important biological roles. Amyloid precursor protein (APP) an Alzheimer's disease (AD)-related gene, recently, we reported the formation of RNA G4 (rG4) at 3'UTR APP mRNA demonstrated its repressive role in translation. Herein, apply rG4-SELEX to develop novel L-RNA aptamer, L-Apt.8f, which binds D-rG4 strongly subnanomolar affinity. We structurally characterize aptamer find...
Abstract Background This study sought to compare the benefits and safety of agents including Cyclin-dependent kinase 4/6 (CDK4/6) inhibitors, phosphoinositide 3-kinase (PI3K)/mammalian target rapamycin (mTOR) histone deacetylase (HDAC) inhibitors as second-line treatments for these patients by conducting a comprehensive systematic review network meta-analysis. Methods The Medline, Embase Cochrane Library databases were searched randomized trials comparing CDK4/6 PI3K/mTOR or HDAC vs. placebo...
Pathological complete response (pCR) has been proven to be related prognosis. pCR can further classified as of the breast (bpCR), axillary lymph nodes (apCR) or both tumors. The aim this study was elucidate outcomes and clinicopathological characteristics associated with different patterns pCR. Patients node-positive disease who received neoadjuvant chemotherapy between August 2009 July 2016 achieved in nodes, were included. Multivariate logistic regression used identify factors Among 271...
Amphiphilic copolymers of stearic acid (SA)-modified Bletilla striata polysaccharides (BSPs-SA) with three different degrees substitution (DSs) were synthesized. The effects DS values on the properties BSPs-SA nanoparticles evaluated. Drug state, cytotoxicity, and histological studies carried out. affinity ability bovine serum albumin (BSA) was also characterized utilizing ultraviolet fluorescence spectroscopy. Besides, bioavailability tissue distribution docetaxel (DTX)-loaded assessed....
Based on target-induced catalytic hairpin assembly and two fluorescent dyes, we have developed a new label-free ratiometric detection method for microRNAs.