A5067832646- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Genetic factors in colorectal cancer
- Cancer Research and Treatments
- Cancer-related gene regulation
- Protein Degradation and Inhibitors
- Bone and Dental Protein Studies
- Genomics and Chromatin Dynamics
- Hippo pathway signaling and YAP/TAZ
- Peptidase Inhibition and Analysis
- Cancer, Lipids, and Metabolism
- Cancer Immunotherapy and Biomarkers
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Bone health and treatments
- Helicobacter pylori-related gastroenterology studies
- Gut microbiota and health
- Endoplasmic Reticulum Stress and Disease
- Histone Deacetylase Inhibitors Research
- Cancer Genomics and Diagnostics
- Liver Disease Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- Colorectal Cancer Treatments and Studies
- Wnt/β-catenin signaling in development and cancer
- Oral microbiology and periodontitis research
Chinese University of Hong Kong
2016-2025
Chinese University of Hong Kong, Shenzhen
2016-2025
Zhejiang University
2011-2024
Fourth People’s Hospital of Jinan
2024
Guiyang Medical University
2024
The Fourth People's Hospital
2024
Affiliated Hospital of Guizhou Medical University
2024
Sunnybrook Health Science Centre
2024
Health Sciences Centre
2024
University of Toronto
2024
Nonalcoholic fatty liver disease (NAFLD)-induced hepatocellular carcinoma (HCC) is an emerging malignancy in the developed world; however, mechanisms that contribute to its formation are largely unknown, and targeted therapy currently not available. Our RNA sequencing analysis of NAFLD-HCC samples revealed squalene epoxidase (SQLE) as top outlier metabolic gene overexpressed patients. Hepatocyte-specific Sqle transgenic expression mice accelerated development high-fat, high-cholesterol...
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A recent mutation analysis suggested that Non-Structural Protein 6 (NSP6) of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a key determinant viral pathogenicity. Here, by transcriptome analysis, we demonstrated inflammasome-related NOD-like receptor signaling was activated in SARS-CoV-2-infected lung epithelial cells and Disease 2019 (COVID-19) patients' tissues. The induction inflammasomes/pyroptosis patients with severe COVID-19 confirmed serological markers....
N6-methyladenosine (m6A) governs the fate of RNAs through m6A readers. Colorectal cancer (CRC) exhibits aberrant modifications and expression regulators. However, how readers interpret oncogenic methylome to promote malignant transformation remains be illustrated.YTH RNA binding protein 1 (Ythdf1) knockout mouse was generated determine effect Ythdf1 in CRC tumorigenesis vivo. Multiomic analysis RNA-sequencing, methylated immunoprecipitation sequencing, YTHDF1 proteomics were performed...
The role of N
Background & AimsGut microbial dysbiosis has pivotal involvement in colorectal cancer (CRC). However, the intratumoral microbiota and its association with CRC progression remain elusive. We aimed to determine community architecture within a neoplasia (CRC or adenoma) contribution carcinogenesis.MethodsWe collected 436 tissue biopsies from patients (n = 36) adenoma 32) (2–6 plus 2–5 adjacent normal tissues per individual). Microbial profiling was performed using 16S ribosomal RNA gene...
Background & AimsPien Tze Huang (PZH) is a well-established traditional medicine with beneficial effects against inflammation and cancer. We aimed to explore the chemopreventive effect of PZH in colorectal cancer (CRC) through modulating gut microbiota.MethodsCRC mouse models were established by azoxymethane plus dextran sulfate sodium treatment or Apcmin/+ mice treated without (270 mg/kg 540 mg/kg). Gut barrier function was determined means intestinal permeability assays transmission...
Background Gastric cancer (GC) is one of the most common worldwide. We analyzed expression m 6 A regulatory genes in GC cohorts and revealed that YTHDF1 was uniquely upregulated as compared with adjacent normal tissues. In this study, we role cells modulation tumor immune microenvironment. Methods Three (cohort 1, n=101; cohort 2, n=278, Cancer Genome Atlas cohort, n=375) were for expression. Function determined cell lines. Role antitumor immunity investigated allograft models. Results...
Non-alcoholic fatty liver disease (NAFLD) is an emerging risk factor of hepatocellular carcinoma (HCC). However, the mechanism and target therapy NAFLD-HCC are still unclear. Here, we identify that N6-methyladenosine (m6A) methyltransferase METTL3 promotes NAFLD-HCC. Hepatocyte-specific Mettl3 knockin exacerbated formation, while knockout exerted opposite effect in mice. Single-cell RNA sequencing revealed suppressed antitumor immune response by reducing granzyme B (GZMB+) interferon...
Immune checkpoint blockade therapy benefits only a small subset of patients with colorectal cancer (CRC), and identification CRC-intrinsic events modulating immune efficacy is an unmet need. We found that AlkB homolog 5 (ALKBH5), RNA N
Carnobacterium maltaromaticum was found to be specifically depleted in female patients with colorectal cancer (CRC). Administration of C. reduces intestinal tumor formation two murine CRC models a female-specific manner. Estrogen increases the attachment and colonization via increasing colonic expression SLC3A2 that binds DD-CPase this bacterium. Metabolomic transcriptomic profiling unveils increased gut abundance vitamin D-related metabolites mucosal activation D receptor (VDR) signaling...
Background & AimsMutant KRAS promotes glutaminolysis, a process that uses steps from the tricarboxylic cycle to convert glutamine α-ketoglutarate and other molecules via glutaminase SLC25A22. This results in inhibition of demethylases epigenetic alterations cells increase proliferation stem cell features. We investigated whether mutant KRAS-mediated glutaminolysis affects epigenomes activities colorectal cancer (CRC) cells.MethodsWe created ApcminKrasG12D mice with intestine-specific...
Cyclooxygenase (COX)-2 is upregulated in hepatocellular carcinoma (HCC). However, the direct causative effect of COX-2 spontaneous HCC formation remains unknown. We thus investigate role and molecular pathogenesis by using liver-specific transgenic (TG) mice. found with elevated inflammatory infiltrates neovessels male TG mice (3/21, 14.3%), but not any WT (0/19). Reduced representation bisulfite sequencing (RRBS) gene expression microarrays were performed tumor non-HCC liver tissues to...
Abstract Cancer stem cells (CSCs) are responsible for tumor progression, recurrence, and drug resistance. To identify genetic vulnerabilities of colon cancer, we performed targeted CRISPR dropout screens comprising 657 Drugbank targets 317 epigenetic regulators on two patient-derived CSC-enriched spheroids. Next-generation sequencing pooled genomic DNAs isolated from surviving yielded therapeutic candidates. We unraveled 44 essential genes spheroids propagation, including key cholesterol...
BackgroundThe outbreak of Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 infection has become a global health emergency. We aim to decipher infected cell types, the consequent host immune response and their interplay in lung COVID-19 patients.MethodsWe analyzed single-cell RNA sequencing (scRNA-seq) data bronchoalveolar lavage fluid (BALF) samples from 10 healthy donors, 6 severe patients 3 mild recovered patients. The expressions receptors (ACE2 TMPRSS2) were examined among...
Abstract Therapeutic targeting of KRAS -mutant colorectal cancer (CRC) is an unmet need. Here, we show that Proprotein Convertase Subtilisin/Kexin type 9 (PSCK9) promotes APC/KRAS-mutant CRC and a therapeutic target. Using patient cohorts, isogenic cell lines transgenic mice, identify de novo cholesterol biosynthesis induced in APC / mutant CRC, accompanied by increased geranylgeranyl diphosphate (GGPP)─a metabolite necessary for activation. PCSK9 the top up-regulated cholesterol-related...
Background and Aims: NASH-HCC is inherently resistant to immune checkpoint blockade, but its tumor microenvironment largely unknown. Approach Results: We applied the imaging mass cytometry construct a spatially resolved single-cell atlas from formalin-fixed paraffin-embedded tissue sections patients with NASH-HCC, virus-HCC (HBV-HCC HCV-HCC), healthy donors. Based on 35 biomarkers, over 750,000 individual cells were categorized into 13 distinct cell types, together expression of key...
Dietary fibers are mainly fermented by the gut microbiota, but their roles in colorectal cancer (CRC) largely unclear. Here, we investigated associations of different with tumorigenesis mice.
KRAS is an important tumor intrinsic factor driving immune suppression in colorectal cancer (CRC). In this study, we demonstrate that SLC25A22 underlies mutant KRAS-induced CRC. immunocompetent male mice and humanized models, knockout inhibits KRAS-mutant CRC growth with reduced myeloid derived suppressor cells (MDSC) but increased CD8+ T-cells, implying the reversion of KRAS-driven immunosuppression. Mechanistically, find plays a central role promoting asparagine, which binds activates SRC...
Abstract Pyogenic liver abscess (PLA) is a common intra-abdominal infection in adults. In this study, we aim to explore demographic and clinical characteristics of PLA focusing on Klebsiella pneumoniae (K. ) induced (KP-PLA) mainland China. A retrospective review medical records from all patients with KP-PLA admitted tertiary teaching hospital over 21-year period (1994–2015) was performed. Among 296 cases confirmed culture-positive data, K. revealed as the predominant pathogen ( n = 189,...