A5027298891- Single-cell and spatial transcriptomics
- Breast Cancer Treatment Studies
- Breast Lesions and Carcinomas
- Neuroinflammation and Neurodegeneration Mechanisms
- Immune cells in cancer
- Cancer and Skin Lesions
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Salivary Gland Tumors Diagnosis and Treatment
- Barrier Structure and Function Studies
- Epigenetics and DNA Methylation
- Prenatal Screening and Diagnostics
- Smoking Behavior and Cessation
- Cancer Immunotherapy and Biomarkers
- MicroRNA in disease regulation
- Lung Cancer Research Studies
- Genomics and Chromatin Dynamics
- Cancer, Lipids, and Metabolism
- Air Quality and Health Impacts
- Hereditary Neurological Disorders
- Ear and Head Tumors
- Cancer Cells and Metastasis
- Air Quality Monitoring and Forecasting
- Genomic variations and chromosomal abnormalities
- Peripheral Neuropathies and Disorders
Chinese University of Hong Kong
2011-2025
University of Hong Kong
2023
First People's Hospital of Foshan
2023
Max Planck Institute for Heart and Lung Research
2019
German Center for Lung Research
2019
John Wiley & Sons (United Kingdom)
2019
Hudson Institute
2019
City University of Hong Kong
2015
Prince of Wales Hospital
2008-2011
Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers
2009
Significance The human placenta is a dynamic and cellular heterogeneous organ, which critical in fetomaternal homeostasis the development of preeclampsia. Previous work has shown that placenta-derived cell-free RNA increases during pregnancy. We applied large-scale microfluidic single-cell transcriptomic technology to comprehensively characterize heterogeneity placentas identified multiple placental cell-type–specific gene signatures. Analysis signature expression maternal plasma enabled...
Objective Therapy-induced tumour microenvironment (TME) remodelling poses a major hurdle for cancer cure. As the majority of patients with hepatocellular carcinoma (HCC) exhibits primary or acquired resistance to antiprogrammed cell death (ligand)-1 (anti-PD-[L]1) therapies, we aimed investigate mechanisms underlying adaptation immune-checkpoint targeting. Design Two immunotherapy-resistant HCC models were generated by serial orthotopic implantation cells through anti-PD-L1-treated...
Abstract The molecular signatures of cells in the brain have been revealed unprecedented detail, yet ageing-associated genome-wide expression changes that may contribute to neurovascular dysfunction neurodegenerative diseases remain elusive. Here, we report zonation-dependent transcriptomic aged mouse endothelial (ECs), which prominently implicate altered immune/cytokine signaling ECs all vascular segments, and functional impacting blood–brain barrier (BBB) glucose/energy metabolism...
Genomic screening uncovered interferon-gamma (IFNγ) pathway defects in tumours refractory to immune checkpoint blockade (ICB). However, its non-mutational regulation and reversibility for therapeutic development remain less understood.
Although abnormal nuclear structure is an important criterion for cancer diagnostics, remarkably little known about its relationship to tumor development. Here we report that loss of lamin B1, a determinant architecture, plays key role in lung cancer. We found B1 levels were reduced patients. Lamin silencing epithelial cells promoted epithelial-mesenchymal transition, cell migration, growth, and metastasis. Mechanistically, show recruits the polycomb repressive complex 2 (PRC2) alter...
Abstract Pharmacological reversal of brain aging is a long-sought yet challenging strategy for the prevention and treatment age-related neurodegeneration, due to diverse cell types complex cellular pathways impacted by process. Here, we report genome-wide transcriptomic signatures in multiple major types, including glial mural cells, systemic glucagon-like peptide-1 receptor (GLP-1R) agonist (GLP-1RA) treatment. The expression changes reversed GLP-1RA encompass both shared type-specific...
Background: Hepatocellular carcinoma (HCC) is a significant global health concern, ranking as the sixth leading cause of cancer-related deaths worldwide. While immune-checkpoint blockade (ICB) has revolutionized cancer treatment, its effectiveness limited by immunosuppressive tumor microenvironment (TME) and diverse etiologies HCC, resulting in response rates below 30%. We have previously identified key transcriptional epigenetic regulators that promote immune exclusion myeloid-mediated...
Tse G M, Tan P‐H, Lacambra M D, Jara‐Lazaro A R, Chan S‐K, Lui P C, Ma T K F, Vong J S, Ng D C H, Shi H‐J & Lam W (2010) Histopathology 56 , 481–488 Papillary lesions of the breast—accuracy core biopsy Aims: To assess accuracy diagnosing papillary breast in needle biopsy. Methods and results: One hundred specimens were reviewed compared with final excisional diagnoses. The discordant stained for oestrogen receptor (ER), cytokeratin (CK) 14 p63, these reclassified based on results....
Abstract BACKGROUND Recent studies have suggested that single-stranded DNA (ssDNA) library preparation can enrich short species from the plasma of healthy individuals, cancer patients, and transplant recipients. Based on previous observations fetal molecules in maternal are shorter than molecules, ssDNA may potentially provide substantial improvement noninvasive prenatal testing. METHODS We tested this hypothesis by comparing sequencing results using 2 types methods a standard...
Background: Cytological diagnosis of mammary papillary lesions is difficult. Aim: To review the previous cytology 23 papillomas and 11 carcinomas specific cytological features that may assist in differentiating these entities. Methods: The preparations were reviewed for: (i) overall cellularity; (ii) epithelial cell ball devoid fibrovascular cores; (iii) background single cells; (iv) fragments their morphology. Results: diagnostic accuracy was 59%, atypical rate 24%, error (combined false...
Human plasma contains RNA transcripts released by multiple cell types within the body. Single-cell transcriptomic analysis allows cellular origin of circulating molecules to be elucidated at high resolution and has been successfully utilized in pregnancy context. We explored application a similar approach develop markers for cancer detection.Single-cell sequencing was performed decipher profiles single cells from hepatocellular carcinoma (HCC) samples. Cell-type-specific were identified used...
Aims: To understand the correlation between expression status of different biological markers in breast cancers elderly. Methods and results: Three hundred ninety‐seven cases were evaluated for hormone receptors [oestrogen (ER) α β, progesterone receptor (PR)], basal [p63, cytokeratin (CK) 5/6 CK14] others (HER2/neu, synaptophysin chromogranin). The rates 60, 29, 25, 6, 14, 8, 28, 17 5%, respectively, these markers. ER PR, chromogranin at any level correlated with low nuclear or tumour...
Cell-free DNA (cfDNA) fragments in maternal plasma contain damage and may negatively impact the sensitivity of noninvasive prenatal testing (NIPT). However, some these damages are potentially reparable. We aimed to recover damaged cfDNA molecules using PreCR repair mix.cfDNA was extracted from 20 samples repaired sequenced by Illumina platform. Size profiles fetal fraction changes were characterized. Targeted sequencing chromosome Y sequences used enrich following repair. Single-molecule...
Abstract With advances in single-cell genomics, molecular signatures of cells comprising the brain vasculature are revealed unprecedented detail 1,2 , yet ageing-associated cell subtype transcriptomic changes which may contribute to neurovascular dysfunction neurodegenerative diseases 3–7 remain elusive. Here, we performed profiling endothelial (EC) young adult and aged mice characterize their genome-wide expression changes. We identified zonation-dependent EC subtypes, with capillary ECs...
Abstract Background Electronic cigarettes (e-cigarettes) have been advertised as a healthier alternative to traditional cigarettes; however, their exact effects on the bronchial epithelium are poorly understood. Air-liquid interface (ALI) culture allows human primary epithelial cells differentiate into (ALI-HBE), providing an in vitro model that simulates biological characteristics of normal epithelium. Methods Single-cell RNA sequencing ALI-HBE was used reveal previously unrecognized...