A5017352422- Carbohydrate Chemistry and Synthesis
- Advanced Biosensing Techniques and Applications
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Gene expression and cancer classification
- Antimicrobial Peptides and Activities
- Alcohol Consumption and Health Effects
- Bacteriophages and microbial interactions
- Liver Disease Diagnosis and Treatment
- Biosensors and Analytical Detection
- Advanced Proteomics Techniques and Applications
- Biochemical and Structural Characterization
- Diverse Musicological Studies
- Chemical Synthesis and Analysis
- Hepatitis C virus research
- Cultural Heritage Materials Analysis
- Enzyme Structure and Function
- Cardiac Arrest and Resuscitation
- RNA modifications and cancer
- Synthesis of Organic Compounds
- Bacterial Genetics and Biotechnology
- Reproductive tract infections research
National Cheng Kung University
2009-2025
National Central University
2013-2023
Fu Jen Catholic University
2016-2023
Taipei Tzu Chi Hospital
2018-2021
Tzu Chi University
2018-2021
Tainan University of Technology
2009-2020
Johns Hopkins University
2006-2018
Johns Hopkins Medicine
2009-2018
Institute of Biological Chemistry, Academia Sinica
2002-2012
National Taiwan University
2002-2012
Chronic liver disease with cirrhosis is the 12th leading cause of death in United States, and alcoholic accounts for approximately half all deaths. alcohol consumption associated intestinal bacterial dysbiosis, yet we understand little about contribution fungi, or mycobiota, to disease. Here have demonstrated that chronic administration increases mycobiota populations translocation fungal β-glucan into systemic circulation mice. Treating mice antifungal agents reduced overgrowth, decreased...
Biofouling on medical devices generally causes adverse complications, such as thrombosis, infection, and pathogenic calcification. Silicone is a widely used material for applications. Its surface modification typically encounters undesirable "hydrophobic recovery", leading to deterioration of engineering. In this study, we developed stable superhydrophilic zwitterionic interface polydimethylsiloxane (PDMS) elastomer by covalent silanization sulfobetaine silane (SBSi) resist nonspecific...
Antimicrobial peptides (AMPs) act either through membrane lysis or by attacking intracellular targets. Intracellular targeting AMPs are a resource for antimicrobial agent development. Several have been identified as peptides; however, the targets of many these remain unknown. In present study, we used an Escherichia coli proteome microarray to systematically identify protein three AMPs: bactenecin 7 (Bac7), hybrid pleurocidin and dermaseptin (P-Der), proline-arginine-rich peptide (PR-39)....
Specific antimicrobial antibodies present in the sera of patients with inflammatory bowel disease (IBD) have been proven to be valuable serological biomarkers for diagnosis/prognosis disease. Herein we describe use a whole Escherichia coli proteome microarray as novel high throughput proteomics approach screen and identify new IBD. Each protein array, which contains 4,256 E. K12 proteins, was screened using individual serum from healthy controls (n = 39) clinically well characterized IBD (66...
Reversible lysine acetylation is one of the most important protein posttranslational modifications that plays essential roles in both prokaryotes and eukaryotes. However, only a few deacetylases (KDACs) have been identified prokaryotes, perhaps part due to their limited sequence homology. Herein, we developed ‘clip-chip’ strategy enable unbiased, activity-based discovery novel KDACs Escherichia coli proteome. In-depth biochemical characterization confirmed YcgC serine hydrolase involving...
Abstract Helicobacter pylori (H. ) infection is a major cause of chronic gastritis and highly related to duodenal ulcer (DU) gastric cancer (GC). To identify H. -related GC biomarkers with high seropositivity in patients, differences levels protein expression between from DU patients were analyzed by isobaric tag for relative absolute quantitation (iTRAQ). In total, 99 proteins showed increased (>1.5-fold) compared 40 these categorized KEGG pathway. The four human disease-related adhesin...
[structure: see text] Ferrocene compounds bearing multiple amido groups selectively bind with the H(2)PO(4)(-) ion over other anions. The one-arm receptors form 1:1 complexes ion, whereas two-arm can accommodate two ions. complexation modes and binding strengths are deduced from NMR, calorimetry, fluorescence, cyclic voltammetry studies. ferrocene hexamide likely exist in synclinal conformations to incorporate tetrahydrofuran molecules
Stem-loop I (SL1) located in the 5' untranslated region of hepatitis C virus (HCV) genome initiates binding to miR-122, a microRNA required for HCV replication. However, proteins that bind SL1 remain elusive. In this study, we employed human proteome microarray, comprised ∼17,000 individually purified full-length, and identified 313 recognize SL1. Eighty-three were annotated as liver-expressing proteins, twelve which known be associated with virus. siRNA-induced silencing eight out 12...
Ectopic ATP synthase on the plasma membrane (eATP synthase) has been found in various cancer types and is a potential target for therapy. However, whether it provides functional role tumor progression remains unclear. Here, quantitative proteomics reveals that cells under starvation stress express higher eATP enhance production of extracellular vesicles (EVs), which are vital regulators within microenvironment. Further results show generates to stimulate EV secretion by enhancing P2X7...
Abstract A novel and convenient route for the synthesis of biologically potent rare L ‐hexose derivatives from D ‐glucose is described. Conversion diacetone‐α‐ ( 14 ) into 1,2:3,5‐di‐ O ‐isopropylidene‐β‐ ‐idofuranose 19 was efficiently carried out in two steps. Orthogonal isopropylidene rearrangement compound led to 1,2:5,6‐di‐ 27 ), which underwent regioselective epimerization at C3 position give ‐talo‐ 3‐functionalized ‐idofuranosyl derivatives. Hydrolysis under acidic conditions...
Fluorescent liposomal nanovesicles (liposomes) are commonly used for lipid research and/or signal enhancement. However, the problem of self-quenching with conventional fluorescent liposomes limits their applications because these must be lysed to detect signals. Here, we developed a nonquenched (NQF)1 liposome by optimizing proportion sulforhodamine B (SRB) encapsulant and lissamine rhodamine B-dipalmitoyl phosphatidylethanol (LRB-DPPE) on surface amplification. Our study showed that 0.3%...
The acid-hydrolyzed fragments of Ganoderma lucidum polysaccharides (GLPS) obtained by Smith degradation were separated size-exclusion chromatography into two major water-soluble fractions: peptidoglycans (GLPS-SF1) and oligosaccharides (GLPS-SF2). Both fractions induced CD69 in human peripheral blood mononuclear cells (hPB-MNCs), they displayed distinct immunomodulating properties. GLPS-SF1, with a molecular weight around 20 kDa, heterogeneous composed glucose/mannose (4:1) that exhibited...
Heterogeneous ribonucleoprotein K (hnRNP K) binds to the 5′ untranslated region of hepatitis C virus (HCV) and is required for HCV RNA replication. The hnRNP binding site on overlaps with sequence recognized by liver-specific microRNA, miR-122. A proteome chip containing ∼17,000 unique human proteins probed miR-122 identified as one strong proteins. In vitro kinetic study showed a nanomolar dissociation constant, in which short pyrimidine-rich residues central 3′ portion were binding. liver...