A5114204446- Viral Infections and Immunology Research
- Immune Cell Function and Interaction
- Eosinophilic Disorders and Syndromes
- Thyroid Disorders and Treatments
- Microscopic Colitis
- Cytokine Signaling Pathways and Interactions
- Cardiac Fibrosis and Remodeling
- Celiac Disease Research and Management
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- IL-33, ST2, and ILC Pathways
- Diabetes and associated disorders
- Cancer Immunotherapy and Biomarkers
- Eosinophilic Esophagitis
- Tryptophan and brain disorders
- Immunodeficiency and Autoimmune Disorders
- CAR-T cell therapy research
- Whipple's Disease and Interleukins
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- Thyroid Cancer Diagnosis and Treatment
- Atherosclerosis and Cardiovascular Diseases
- Immune cells in cancer
- Inflammatory Bowel Disease
- Drug-Induced Hepatotoxicity and Protection
Johns Hopkins University
2016-2025
Johns Hopkins Medicine
2015-2025
University of Baltimore
2007-2021
University of Maryland, Baltimore
2019-2020
Massachusetts General Hospital
2019-2020
Carleton College
2020
Bloomberg (United States)
2020
University of South Carolina
2020
Kent State University
2019
University of Maryland, College Park
2019
Rationale : One-third of myocarditis cases progresses to dilated cardiomyopathy (DCM), but the mechanisms controlling this process are largely unknown. CD4 + T helper (Th)17 cells have been implicated in pathogenesis autoimmune diseases, role Th17-produced cytokines during inflammation-induced cardiac remodeling has not previously studied. Objective We examined importance interleukin (IL)-17A progression DCM using a mouse model. Methods and Results Immunization mice with myocarditogenic...
Inflammatory dilated cardiomyopathy (DCMi) is a major cause of heart failure in individuals below the age 40. We recently reported that IL-17A required for development DCMi. show novel pathway connecting IL-17A, cardiac fibroblasts (CFs), GM-CSF, and heart-infiltrating myeloid cells with pathogenesis Il17ra−/− mice were protected from DCMi, this was associated significantly diminished neutrophil Ly6Chi monocyte/macrophage (MO/MΦ) infiltrates. Depletion MO/MΦ also Mechanistically, stimulated...
Antibodies to thyroglobulin (Tg), thyroperoxidase (TPO), and TSH receptor (TSH-R) are prevalent in autoimmune thyroid diseases. We aimed assess whether females with Graves disease or Hashimoto thyroiditis more likely than age-matched controls have antibodies before clinical diagnosis measure the timing of antibody seroconversion.This was a nested case-control study using Department Defense Serum Repository Medical Surveillance System, 1998-2007. assessed serum 522 female, active-duty,...
Inflammatory dilated cardiomyopathy (DCMi) is a major cause of heart failure in children and young adults. DCMi develops up to 30% myocarditis patients, but the mechanisms involved disease progression are poorly understood. Patients with eosinophilia frequently develop cardiomyopathies. In this study, we used experimental autoimmune (EAM) model determine role eosinophils DCMi. Eosinophils were dispensable for induction required Eosinophil-deficient ΔdblGATA1 mice, contrast WT showed no signs...
Immune checkpoint inhibitors (ICIs) are an effective therapy for various cancers; however, they can induce immune-related adverse events (irAEs) as a side effect. Myocarditis is uncommon, but fatal, irAE caused after ICI treatments. Currently, the mechanism of ICI-associated myocarditis unclear. Here, we show development in A/J mice induced by anti-PD-1 monoclonal antibody (mAb) administration alone without tumor cell inoculation, immunization, or viral infection. Mice with have increased...
Myocarditis and myopericarditis may occur after COVID-19 vaccination with an incidence of two to twenty cases per 100,000 individuals, but underlying mechanisms related disease onset progression remain unclear. Here, we report a case following the first dose mRNA-1273 vaccine in young man who had history mild three months before vaccination. The patient presented chest pain, elevated troponin I level, electrocardiogram abnormality. His endomyocardial biopsy revealed diffuse CD68+ cell...
The involvement of macrophages (MΦs) in Th17-cell responses is still poorly understood. While neutrophils are thought to be the predominant effector responses, IL-17 also known induce myelotropic chemokines and growth factors. Other T-cell-derived cytokines non-classical functions, suggesting that sigxnaling may similarly elicit unique MΦ functions. Here, we characterized expression subunits receptor on primary murine MΦs from different anatomical compartments. greatest receptors was...
Myocarditis is a leading cause of sudden cardiac failure in young adults. Natural killer (NK) cells, subset the innate lymphoid cell compartment, are protective viral myocarditis. Herein, we demonstrated that these qualities extend to suppressing autoimmune inflammation. Experimental myocarditis (EAM) was initiated BALB/c mice by immunization with myocarditogenic peptide. During EAM, activated NK cells secreted interferon γ, perforin, and granzyme B, expressed CD69, tumor necrosis...
Cardiac manifestations are a major cause of morbidity and mortality in patients with eosinophil-associated diseases. Eosinophils thought to play pathogenic role myocarditis. We investigated the pathways that recruit eosinophils heart using model eosinophilic myocarditis, which experimental autoimmune myocarditis (EAM) is induced IFNγ-/- IL-17A-/- mice. Two conditions necessary for efficient eosinophil trafficking heart: high eotaxin (CCL11, CCL24) expression receptor CCR3 by eosinophils....
Two types of monocytes, Ly6Chi and Ly6Clo, infiltrate the heart in murine experimental autoimmune myocarditis (EAM). We discovered a role for cardiac fibroblasts facilitating monocyte-to-macrophage differentiation both Ly6Clo cells, allowing these macrophages to perform divergent functions progression. During acute phase EAM, IL-17A is highly abundant. It signals through attenuate efferocytosis monocyte-derived (MDMs) simultaneously prevents differentiation. demonstrated an inverse clinical...
Specific antimicrobial antibodies present in the sera of patients with inflammatory bowel disease (IBD) have been proven to be valuable serological biomarkers for diagnosis/prognosis disease. Herein we describe use a whole Escherichia coli proteome microarray as novel high throughput proteomics approach screen and identify new IBD. Each protein array, which contains 4,256 E. K12 proteins, was screened using individual serum from healthy controls (n = 39) clinically well characterized IBD (66...
Transcriptomic studies hold great potential towards understanding the human aging process. Previous transcriptomic have identified many genes with age-associated expression levels; however, small samples sizes and mixed cell types often make these results difficult to interpret. Using profiles in CD14+ monocytes from 1,264 participants of Multi-Ethnic Study Atherosclerosis (aged 55–94 years), we 2,704 differentially expressed chronological age (false discovery rate, FDR ≤ 0.001). We further...
CD4(+) T cells play a central role in inflammatory heart disease, implicating cytokine product associated with Th cell effector function as necessary mediator of this pathophysiology. IFN-γ-deficient mice developed severe experimental autoimmune myocarditis (EAM), which are immunized cardiac myosin peptide, whereas IL-17A-deficient were protected from progression to dilated cardiomyopathy. We generated IFN-γ(-/-)IL-17A(-/-) assess whether IL-17 signaling was responsible for the EAM...
Introduction Complete Freund's Adjuvant (CFA) emulsified with an antigen is a widely used method to induce autoimmune disease in animal models, yet the contribution of CFA immune response not well understood. We compared effectiveness Incomplete (IFA) or TiterMax Gold (TMax) experimental myocarditis (EAM) male mice. Methods EAM was induced A/J, BALB/c, and IL6KO BALB/c mice by injection myocarditogenic peptide CFA, IFA, TMax on days 0 7. severity analyzed histology day 21. In addition,...
The causative effect of GM-CSF produced by cardiac fibroblasts to development heart failure has not been shown. We identified the pathological GM-CSF-producing fibroblast subset and specific deletion IL-17A signaling these cells attenuated inflammation failure. describe here CD45- CD31- CD29+ mEF-SK4+ PDGFRα+ Sca-1+ periostin+ (Sca-1+ ) as main producer in both experimental autoimmune myocarditis myocardial infarction mouse models. Specific ablation (PostnCre Il17rafl/fl protected mice from...
About one in three persons with a schizophrenia related disorder (SRD) have elevated anti-gliadin IgG antibodies (AGA). This AGA positive (AGA+) subgroup of SRD clinically has higher burden negative symptoms and are associated high functional impairments lack effective therapeutics. Alterations T cells been demonstrated SRD, we previously shown regulatory (Tregs) increased correlate fewer compared healthy controls. To further elucidate the role immune system AGA+ pathology, investigated...
Schizophrenia and related disorders (SRD) are characterized by positive negative symptoms, such as anhedonia avolition. There no current FDA approved treatments for which is a critical gap in our treatment of people with SRDs, since they major determinant functional impairment. An emerging literature suggests that SRDs have relationship immune function inflammation. Recently an SRD subgroup high inflammation elevated levels anti-gliadin antibodies (AGA) immunoglobulin G type (IgG) ha s been...
Summary In patients with anti-neutrophil cytoplasmic antibodies (ANCA)-associated vasculitis, indirect immunofluorescence (IF) distinguishes between (C-ANCA) and perinuclear (P-ANCA) neutrophil staining patterns. primary systemic vasculitis such as Wegener's granulomatosis, microscopic polyangiitis Churg–Strauss syndrome, these IF patterns correspond broadly to the two major antigens: C-ANCA pattern is associated generally serine protease 3 (PR3) P-ANCA myeloperoxidase (MPO). However, some...
Background and Aims Immune-mediated, drug-induced liver injury (DILI) triggered by drug haptens is more prevalent in women than men. However, mechanisms responsible for this sex bias are not clear. Immune regulation CD4+CD25+FoxP3+ regulatory T-cells (Tregs) 17β-estradiol crucial the pathogenesis of cancer autoimmunity. Therefore, we investigated their role a mouse model immune-mediated DILI. Methods To DILI, immunized BALB/c, BALB/cBy, IL-6–deficient, castrated BALB/c mice with...
Innate lymphoid cells (ILC) are a subset of leukocytes with properties that lack antigen specific receptors. They can be stimulated by and exert their effect via cytokine axes, whereas Natural Killers (NK) the only known cytotoxic member this family. ILCs considered key in linking innate adaptive response physiologic pathologic environments. In study, we investigated non-cytotoxic cardiac physiologic, inflammatory, ischemic conditions. We found healthy humans mice, predominantly type...
<h3>Background:</h3> Approximately one-third of people with schizophrenia have elevated levels antigliadin antibodies the immunoglobulin G type (AGA IgG) — a higher rate than seen in healthy controls. We performed first double-blind clinical trial gluten-free versus gluten-containing diets subset patients who were positive for AGA IgG. <h3>Methods:</h3> In this pilot feasibility study, 16 participants or schizoaffective disorder had IgG (≥ 20 U) but negative celiac disease admitted to an...