A5010903919- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Viral Infections and Immunology Research
- RNA modifications and cancer
- Cardiovascular Effects of Exercise
- Cardiomyopathy and Myosin Studies
- Eosinophilic Disorders and Syndromes
- Cardiac Structural Anomalies and Repair
- RNA Research and Splicing
- Single-cell and spatial transcriptomics
- Circular RNAs in diseases
- Transplantation: Methods and Outcomes
- Connective tissue disorders research
- Advanced Glycation End Products research
- Cardiac electrophysiology and arrhythmias
- Cardiovascular Function and Risk Factors
- Molecular Biology Techniques and Applications
- Aortic Disease and Treatment Approaches
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Aortic aneurysm repair treatments
- Cancer Cells and Metastasis
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Systemic Lupus Erythematosus Research
- Sports injuries and prevention
Cleveland Clinic
2013-2025
Johns Hopkins University
2015-2024
Johns Hopkins Medicine
2015-2024
Cleveland Clinic Lerner College of Medicine
2023
Duke University
2021
Sidney Kimmel Comprehensive Cancer Center
2017-2018
Johns Hopkins Hospital
2006-2017
Daewoong Pharmaceutical (South Korea)
2017
University of Alabama at Birmingham
2017
University of Pennsylvania
2017
Aortic aneurysm and dissection are manifestations of Marfan syndrome (MFS), a disorder caused by mutations in the gene that encodes fibrillin-1. Selected MFS reflect excessive signaling transforming growth factor-beta (TGF-beta) family cytokines. We show aortic mouse model is associated with increased TGF-beta can be prevented antagonists such as TGF-beta-neutralizing antibody or angiotensin II type 1 receptor (AT1) blocker, losartan. AT1 antagonism also partially reversed noncardiovascular...
Purpose Contrary to the extensive data accumulated regarding pancreatic carcinogenesis, clinical and molecular features characteristic of advanced stage (stage III IV) disease are unknown. A comprehensive study cancers from patients who have succumbed their has potential greatly expand our understanding most lethal this identify novel areas for intervention. Materials Methods Rapid autopsies were performed on 76 with documented cancer. The histologic end determined correlated at initial...
Recent controversies surrounding prostate cancer overtreatment emphasize the critical need to delineate molecular features associated with progression lethal metastatic disease. Here, we have used whole-genome sequencing and pathological analyses characterize cell clone in a patient who died of cancer. We tracked evolution from primary metastases through samples collected during disease at time death. Surprisingly, these revealed that arose small, relatively low-grade focus tumor, not bulk,...
The diagnosis of arrhythmogenic right ventricular cardiomyopathy (ARVC) can be challenging because the clinical presentation is highly variable and genetic penetrance often low.To determine whether a change in distribution desmosomal proteins used as sensitive specific diagnostic test for ARVC, we performed immunohistochemical analysis human myocardial samples.We first tested myocardium from 11 subjects with ARVC; these samples, 8 had gene mutations. We also obtained at autopsy 10 no or...
Purpose To determine the utility of cardiac magnetic resonance (MR) T1 mapping for quantification diffuse myocardial fibrosis compared with standard endomyocardial biopsy. Materials and Methods This HIPAA-compliant study was approved by institutional review board. Cardiomyopathy patients were retrospectively identified who had undergone biopsy MR at one institution during a 5-year period. Forty-seven (53% male; mean age, 46.8 years) diagnostic Thirteen healthy volunteers (54% 38.1 underwent...
Enterohemorrhagic E. coli (EHEC) causes over 70,000 episodes of foodborne diarrhea annually in the USA. The early sequence events which precede life-threatening hemorrhagic colitis and hemolytic uremic syndrome are not fully understood due to initial asymptomatic phase disease lack a suitable animal model. aim this study was determine molecular interaction between EHEC human colonic epithelium.Human colonoids derived from adult proximal stem cells were developed into monolayers...
Large scale human genetic studies require technologies for generating millions of genotypes with relative ease but also at a reasonable cost and high accuracy. We describe highly parallel method genotyping single nucleotide polymorphisms (SNPs), using generic high-density oligonucleotide arrays that contain thousands preselected 20-mer tags. First, marker-specific primers are used in PCR amplifications genomic regions containing SNPs. Second, the amplification products as templates base...
A critical clinical application of the Human Genome Project is to identify functional variation in genes related disease or responses xenobiotics. This study moved toward that goal by combining polymorphism detection with assays for therapeutic target gene cyclooxygenase 1 (COX-1). Cyclooxygenase (prostaglandin endoperoxide G/H synthase [PTGS1]) catalyzes metabolism arachidonic acid prostaglandin H(2), which subsequently metabolized thromboxane A(2).Thirty-eight healthy participants were...
Abstract BACKGROUND. Sunitinib malate is a novel multitargeted receptor tyrosine kinase inhibitor with established efficacy in the treatment of metastatic renal cell carcinoma and imatinib‐resistant gastrointestinal stromal tumor. This report describes development heart failure cancer patients who received this agent. METHODS. A retrospective study was conducted at M. D. Anderson Cancer Center during 1‐year period on sunitinib developed failure. RESULTS. During 2006, 6 224 (2.7%) (HF) that...
Sustained pressure overload induces pathological cardiac hypertrophy and dysfunction. Oxidative stress linked to nitric oxide synthase (NOS) uncoupling may play an important role. We tested whether tetrahydrobiopterin (BH4) can recouple NOS reverse preestablished advanced hypertrophy, fibrosis, dysfunction.C57/Bl6 mice underwent transverse aortic constriction for 4 weeks, increasing mass (190%) diastolic dimension (144%), lowering ejection fraction (-46%), triggering oxidative stress. Oral...
Cellular identity and differentiation are determined by epigenetic programs. The characteristics of these programs in normal human mammary epithelium their similarity to those stem cells unknown. To begin investigating issues, we analyzed the DNA methylation gene expression profiles distinct subpopulations epithelial using MSDK (methylation-specific digital karyotyping) SAGE (serial analysis expression). We identified discrete cell-type state-specific patterns that were maintained a subset...
Abstract Purpose: A comprehensive comparison of biomarker expression between patients' primary breast carcinoma (PBC) and their metastatic carcinomas (MBC) has not been done. Experimental Design: We did rapid autopsies (postmortem intervals, 1-4 hours) on 10 consenting patients who died MBC. constructed single-patient tissue microarrays from the archived PBC multiple different MBCs harvested at autopsy, which were immunohistochemically labeled for biomarkers. Methylation gene promoters was...