A5009195048- Bladder and Urothelial Cancer Treatments
- Urinary and Genital Oncology Studies
- Cancer Immunotherapy and Biomarkers
- Metastasis and carcinoma case studies
- Genital Health and Disease
- Pancreatic and Hepatic Oncology Research
- Immunotherapy and Immune Responses
- Urologic and reproductive health conditions
- Renal and related cancers
- Urological Disorders and Treatments
- Cancer Cells and Metastasis
- Renal cell carcinoma treatment
- Neuroendocrine Tumor Research Advances
- Cervical Cancer and HPV Research
- Advanced Battery Materials and Technologies
- Advancements in Battery Materials
- Sarcoma Diagnosis and Treatment
- Thyroid Cancer Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Hedgehog Signaling Pathway Studies
- Colorectal and Anal Carcinomas
- Chromatin Remodeling and Cancer
- Prostate Cancer Treatment and Research
- Cancer Genomics and Diagnostics
- Cancer Diagnosis and Treatment
Johns Hopkins Hospital
2009-2020
Johns Hopkins University
2010-2019
Johns Hopkins Medicine
2005-2019
Indian Institute of Integrative Medicine
2009-2018
Academy of Scientific and Innovative Research
2013-2018
Dr. Shakuntala Misra National Rehabilitation University
2018
Dr. Mohan's Diabetes Specialities Centre
2018
Indian Institute of Technology Roorkee
2012-2018
Avantha Centre for Industrial Research & Development
2018
University of Jammu
2012-2018
Although many human cancers such as melanoma express tumor antigens recognized by T cells, host immune responses often fail to control growth for yet unexplained reasons. Here, we found a strong association between melanocyte expression of B7-H1 (PD-L1), an immune-inhibitory molecule, and the presence tumor-infiltrating lymphocytes (TILs) in melanocytic lesions: 98% B7-H1(+) tumors were associated with TILs compared only 28% B7-H1(-) tumors. Indeed, melanocytes almost always localized...
Poorly differentiated neuroendocrine carcinomas (NECs) of the pancreas are rare malignant neoplasms with a poor prognosis. The aim this study was to determine clinicopathologic and genetic features poorly NECs compare them other types pancreatic neoplasms. We investigated alterations KRAS, CDKN2A/p16, TP53, SMAD4/DPC4, DAXX, ATRX, PTEN, Bcl2, RB1 by immunohistochemistry and/or targeted exomic sequencing in surgically resected specimens 9 small cell NECs, 10 large 11 well-differentiated...
Pancreatic ductal adenocarcinoma (PDAC) is considered a "nonimmunogenic" neoplasm. Single-agent immunotherapies have failed to demonstrate significant clinical activity in PDAC and other tumors, part due complex tumor microenvironment (TME) that provides formidable barrier immune infiltration function. We designed neoadjuvant adjuvant trial comparing an irradiated, granulocyte-macrophage colony-stimulating factor (GM-CSF)-secreting, allogeneic vaccine (GVAX) given as single agent or...
Surgical resection provides the only possibility of cure for pancreas cancer. A standard adjuvant approach has not been established. We tested safety and efficacy a granulocyte-macrophage colony-stimulating factor (GM-CSF)-based immunotherapy administered in patients with resected pancreatic adenocarcinoma.A single institution phase II study 60 adenocarcinoma was performed. Each treatment consisted total 5 × 108 GM-CSF-secreting cells distributed equally among 3 lymph node regions. The first...
Cancers comprise a heterogeneous group of human diseases. Unifying characteristics include unchecked abilities tumor cells to proliferate and spread anatomically, the presence clonal advantageous genetic changes. However, universal highly specific markers are unknown. Herein, we report widespread long interspersed element-1 (LINE-1) repeat expression in cancers. We show that nearly half all cancers immunoreactive for LINE-1-encoded protein. LINE-1 protein is common feature many types...
Abstract Recent evidence suggests that blockade of aberrant Hedgehog signaling can be exploited as a therapeutic strategy for pancreatic cancer. Our previous studies using the prototype small-molecule antagonist cyclopamine had shown striking inhibition systemic metastases on in spontaneously metastatic orthotopic xenograft models. Cyclopamine is natural compound with suboptimal pharmacokinetics, which impedes clinical translation. In present study, novel, orally bioavailable inhibitor,...
The goal of this study was to evaluate prospectively the engraftment rate, factors influencing engraftment, and predictability clinical outcome low-passage xenografts from patients with resectable pancreatic ductal adenocarcinoma (PDA) establish a bank PDA xenografts.Patients scheduled for resection at Johns Hopkins Hospital were eligible. Representative pieces tumor implanted in nude mice. status SMAD4 gene content tumor-generating cells determined by immunohistochemistry. Gene expression...
The histologic distinction between high-grade prostate cancer and infiltrating urothelial may be difficult, has significant implications because each disease treated very differently (ie, hormone therapy for chemotherapy cancer). Immunohistochemistry of novel established prostatic markers using tissue microarrays (TMAs) were studied. Prostatic studied included: prostate-specific antigen (PSA), prostein (P501s), membrane (PSMA), NKX3.1 (an androgen-related tumor suppressor gene), proPSA...
Curcumin or diferuloylmethane is a yellow polyphenol extracted from the rhizome of turmeric (Curcuma longa). A large volume (several hundreds) published reports has established anticancer and chemopreventative properties curcumin in preclinical models every known major cancer type. Nevertheless, clinical translation been significantly hampered due to its poor systemic bioavailability, which mandates that patients consume up 8 10 g free drug orally each day achieve detectable levels...
Abstract NOTCH1 mutations have been reported to occur in 10% 15% of head and neck squamous cell carcinomas (HNSCC). To determine the significance these mutations, we embarked upon a comprehensive study NOTCH signaling cohort 44 HNSCC tumors 25 normal mucosal samples through set expression, copy number, methylation, mutation analyses. Copy number increases were identified pathway genes, including ligand JAG1. Gene analysis defined differential expression relative tissues. Analysis individual...
Merkel Cell Virus (MCV) is a newly discovered polyomavirus, recently found in rare skin cancer, cell carcinoma (MCC). However, MCV has also been detected some normal tissue samples. We tested and compared the relative quantity of set diverse human samples with MCC The levels MCCs were over 60 times higher than highest values all other tissues. Low quantities independently malignant or benign histologic status. Higher virus upper aerodigestive tract, digestive system, saliva to lung...
The aggressiveness of pancreatic ductal adenocarcinoma (PDA) is characterized by its high metastatic potential and lack effective therapies, which the result a understanding mechanisms involved in promoting PDA metastases. We identified Annexin A2 (ANXA2), member family calcium-dependent phospholipid binding proteins, as new molecule that promotes invasion found ANXA2 to be PDA-associated antigen recognized post-treatment sera patients who demonstrated prolonged survival following treatment...
Tyrosine kinase inhibitors (TKI) targeting angiogenesis via inhibition of the vascular endothelial growth factor pathway have changed medical management metastatic renal cell carcinoma. Although treatment with TKIs has shown clinical benefit, these drugs will eventually fail patients. The potential mechanisms resistance to are poorly understood. To address this question, we obtained an excisional biopsy a skin metastasis from patient clear carcinoma who initially had response sunitinib and...
Distinguishing invasive high-grade urothelial carcinoma (UC) from other carcinomas occurring in the genitourinary tract may be difficult. The differential diagnosis includes prostatic adenocarcinoma, spread an anal squamous cell (SCC), or a uterine cervical SCC. In terms of metastatic UC, most common problem is differentiating UC to lung primary pulmonary Immunohistochemical analysis (IHC) for GATA binding protein 3 (GATA3), thrombomodulin (THROMBO), and uroplakin III was performed on tissue...
Despite the importance of recognizing neuroendocrine differentiation when diagnosing tumors thoracic cavity, sensitivity traditional markers is suboptimal, particularly for high-grade carcinomas such as small cell lung carcinoma and large carcinoma. To increase sensitivity, are routinely ordered panels multiple immunostains where any single positive marker regarded sufficient evidence differentiation. Insulinoma-associated protein 1 (INSM1) a well-validated transcription factor that has only...
Background New markers to distinguish benign reactive glands from infiltrating ductal adenocarcinoma of the pancreas are needed. Design The gene expression patterns 24 surgically resected primary adenocarcinomas were compared with 18 non-neoplastic samples using Affymetrix U133 Plus 2.0 Arrays and Gene Logic GeneExpress Software System. fragments 4 genes (annexin A8, claudin 18, CXCL5, S100 A2) selected found be highly expressed in when normal tissues. protein these was examined...