A5048533064- Epigenetics and DNA Methylation
- Cervical Cancer and HPV Research
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Ovarian cancer diagnosis and treatment
- Head and Neck Cancer Studies
- Cancer-related gene regulation
- Cancer Genomics and Diagnostics
- Kruppel-like factors research
- Molecular Biology Techniques and Applications
- Bladder and Urothelial Cancer Treatments
- Macrophage Migration Inhibitory Factor
- Salivary Gland Disorders and Functions
- Oral microbiology and periodontitis research
- Ferroptosis and cancer prognosis
- Cancer-related Molecular Pathways
- Gut microbiota and health
- Health, Environment, Cognitive Aging
- Global Cancer Incidence and Screening
- MicroRNA in disease regulation
- Renal cell carcinoma treatment
- Urological Disorders and Treatments
- Plasma Applications and Diagnostics
- Esophageal Cancer Research and Treatment
- Birth, Development, and Health
Johns Hopkins University
2012-2024
Johns Hopkins Medicine
2012-2024
University of Puerto Rico System
2010-2020
University of Baltimore
2010-2018
University of Puerto Rico at Río Piedras
2014
Indiana University School of Medicine
2012-2014
Pontificia Universidad Católica de Chile
2013
Universidad de La Frontera
2013
Institute of Physiology and Basic Medicine
2012
Vaccine Research Institute of San Diego
2012
Plasma is an ionised gas that typically generated in high-temperature laboratory conditions. However, recent progress atmospheric plasmas has led to the creation of cold with ion temperature close room temperature.Both in-vitro and in-vivo studies revealed selectively kill cancer cells.We show that: (a) plasma application eradicates cells vitro without damaging normal cells; (b) significantly reduces tumour size vivo. It shown reactive oxygen species metabolism oxidative stress responsive...
// Rafael Guerrero-Preston 1, 2 , Filipa Godoy-Vitorino 3 Anne Jedlicka 4 Arnold Rodríguez-Hilario Herminio González Jessica Bondy 1 Fahcina Lawson Oluwasina Folawiyo Christina Michailidi Amanda Dziedzic Rajagowthamee Thangavel 5 Tal Hadar Maartje G. Noordhuis 6 William Westra 7 Wayne Koch David Sidransky Department of Otolaryngology and Head Neck Surgery, Johns Hopkins University School Medicine, Baltimore, Maryland, USA Obstetrics Gynecology, Puerto Rico San Juan, Natural...
The treatment of locoregional recurrence (LRR) head and neck squamous cell carcinoma (HNSCC) often requires a combination surgery, radiation therapy and/or chemotherapy. Survival outcomes are poor the morbid. Cold atmospheric plasma (CAP) is an ionized gas produced at room temperature under laboratory conditions. We have previously demonstrated that with CAP jet device selectively targets cancer cells using in vitro melanoma vivo bladder models. In present study, we wished to examine...
Environmental exposures in-utero may alter the epigenome, thus impacting chromosomal stability and gene expression. We hypothesized that in utero to maternal smoking perfluoroalkyl compounds (PFCs) are associated with global DNA hypomethylation umbilical cord serum. Our objective was determine if methylation could be used as a biomarker of PFCs. Using an ELISA-based method, quantified serum from 30 newborns high (>10 ng/ml, mean 123.8 ng/ml), low (range 1-10 1.6 ng/ml) very (<1 0.06 cotinine...
Merkel Cell Virus (MCV) is a newly discovered polyomavirus, recently found in rare skin cancer, cell carcinoma (MCC). However, MCV has also been detected some normal tissue samples. We tested and compared the relative quantity of set diverse human samples with MCC The levels MCCs were over 60 times higher than highest values all other tissues. Low quantities independently malignant or benign histologic status. Higher virus upper aerodigestive tract, digestive system, saliva to lung...
Tumor suppressor genes (TSGs) are commonly inactivated by somatic mutation and/or promoter methylation; yet, recent high-throughput genomic studies have not identified key TSGs both mechanisms. We pursued an integrated molecular analysis based on methylation binding domain sequencing (MBD-seq), 450K Methylation arrays, whole exome sequencing, and genome gene expression arrays in primary head neck squamous cell carcinoma (HNSCC) tumors matched uvulopalatopharyngoplasty tissue samples (UPPPs)....
Purpose: To establish a novel panel of cancer-specific methylated genes for cancer detection and prognostic stratification early-stage non-small cell lung (NSCLC).Experimental Design: Identification differentially regions (DMR) was performed with bumphunter on "The Cancer Genome Atlas (TCGA)" dataset, clinical utility assessed using quantitative methylation-specific PCR assay in multiple sets primary NSCLC body fluids that included serum, pleural effusion, ascites samples.Results: A...
Abstract Differentially methylated oral squamous cell carcinoma (OSCC) biomarkers, identified in vitro and validated well-characterized surgical specimens, have shown poor clinical correlation cohorts with different risk profiles. To overcome this lack of relevance, we used the HumanMethylation27 BeadChip, publicly available methylation expression array data, quantitative specific PCR to uncover differential OSCC samples heterogeneous A two stage design consisting discovery prevalence...
Cisplatin was shown to induce the ataxia telangiectasia mutated (ATM)-dependent phosphorylation of tumor protein p63 isoform, (ΔNp63α), leading a transcriptional regulation specific genes implicated in control cell death squamous carcinoma (SCC) cells. We previously observed that cisplatin-induced phosphorylated (p)-ΔNp63α transcriptionally regulates expression microRNAs (miRNAs) SCC found here cisplatin exposure cells led modulation members autophagic pathway, such as Atg1/Ulk1, Atg3,...
// Rafael Guerrero-Preston 1, 2 , James Robert White 3 Filipa Godoy-Vitorino 4 Arnold Rodríguez-Hilario Kelvin Navarro Herminio González Christina Michailidi 1 Anne Jedlicka 5 Sierra Canapp Jessica Bondy Amanda Dziedzic Barbara Mora-Lagos 6 Gustavo Rivera-Alvarez Carmen Ili-Gangas 6, 7 Priscilla Brebi-Mieville William Westra Wayne Koch Hyunseok Kang 8 Luigi Marchionni Young Kim 9 and David Sidransky Department of Otolaryngology Head Neck Surgery, Johns Hopkins University, School Medicine,...
In addition to genetic changes, the occurrence of epigenetic alterations is associated with accumulation both and events that promote development progression human cancer. Previously, we reported a set candidate genes comprise part emerging "cancer methylome". present study, first tested 23 for promoter methylation in small number primary colon tumor tissues controls. Based on these results, then examined frequency Oncostatin M receptor-beta (OSMR) larger tissue stool DNA samples collected...
Methylation alterations of CpG islands, island shores and first exons are key events in the formation progression human cancer, an increasing number differentially methylated regions genes have been identified breast cancer. Recent studies cancer methylome using deep sequencing microarray platforms providing a novel insight on different roles aberrant methylation plays molecular subtypes Accumulating evidence from subset suggests that promoter tumor-suppressor associated with can be...
Cisplatin chemoresistance is a clinical problem that leads to treatment failure in various human epithelial cancers. Members of tumor protein (TP) p53 family play critical roles the multiple molecular mechanisms underlying cells. However, in-depth cellular response cisplatin-induced cell death are still under thorough investigation. We previously showed squamous carcinoma (SCC) cells exposed cisplatin display an ATM-dependent phosphorylation ΔNp63α, leading specific function phosphorylated...
// Francesca Pirini 1, * , Sassan Noazin 2, Martha H. Jahuira-Arias 3, 4, Sebastian Rodriguez-Torres 3 Leah Friess Christina Michailidi Jaime Cok 5 Juan Combe 6 Gloria Vargas 7 William Prado 8 Ethan Soudry Jimena Pérez Tikki Yudin Andrea Mancinelli Helen Unger Carmen Ili-Gangas 9, 10 Priscilla Brebi-Mieville Douglas E. Berg 11, 12 Masamichi Hayashi 13 David Sidransky Robert Gilman 4 Rafael Guerrero-Preston 14 1 Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura...
Urothelial cell carcinoma (UCC) is the second most common genitourinary malignant disease in USA, and tobacco smoking major known risk factor for UCC development. Exposure to carcinogens, such as those contained smoke, directly or indirectly damage DNA, causing mutations, chromosomal deletion events epigenetic alterations UCC. Molecular studies have shown that chromosome 9 P53, RAS, RB PTEN mutations are among frequent Recent suggested continuous carcinogen exposure drives enhances selection...
Persons of Hispanic/Latino descent may represent different ancestries, ethnic and cultural groups countries birth. In the U.S., population is projected to constitute 29% by 2060. A personalized approach focusing on individual variability in genetics, environment, lifestyle socioeconomic determinants health advance understanding some major factors contributing disparities experienced Hispanics/Latinos other thus leading new strategies that improve care outcomes. However, there are gaps our...
Clinically useful molecular tools to triage women for a biopsy upon referral colposcopy are not available. We aimed develop panel detect cervical intraepithelial neoplasia (CIN) grade 2 or higher lesions (CIN2+) in with abnormal cytology and high-risk HPV (HPV+). tested biomarker epithelium DNA obtained from 211 evaluated cancer clinic Chile 2006 2008. Results were verified prospective cohort of 107 Puerto Rico 2013 2015. Promoter methylation ZNF516, FKBP6, INTS1 discriminated brush samples...
Differentially methylated regions (DMRs) can be used as head and neck squamous cell carcinoma (HNSCC) diagnostic, prognostic therapeutic targets in precision medicine workflows. DNA from 21 HNSCC 10 healthy oral tissue samples was hybridized to a genome-wide tiling array identify DMRs discovery cohort. Downstream analyses identified differences promoter methylation patterns oral, laryngeal oropharyngeal anatomical associated with tumor differentiation, nodal involvement survival. Genome-wide...
Late‑stage cancers lack effective treatment, underscoring the need for early diagnosis to improve prognosis and decrease mortality rates. Molecular markers, such as DNA methylation, offer promise in cancer detection. The present study compared commercial kits analyzing from cervical liquid cytology samples screening. Rapid bisulfite conversion using silica spin‑columns magnetic beads were assessed against standard extraction methods profiling methylation quantitative methylation‑specific...
Cervical cancer is a major health concern among women in Latin America due to its high incidence and mortality. Therefore, the discovery of molecular markers for cervical screening triage imperative. The aim this study was use genome wide DNA methylation approach identify novel biomarkers cancer. from normal mucosa tissue samples Chile enriched with Methylated Immunoprecipitation (MeDIP), hybridized oligonucleotide microarrays analyzed stringent bioinformatics pipeline differentially...
Poor prognosis in gallbladder cancer is due to late presentation of the disease, lack reliable biomarkers for early diagnosis and limited targeted therapies. Early diagnostic markers novel therapeutic targets can significantly improve clinical management cancer. Proteomic analysis four cell lines based on invasive property (non-invasive highly invasive) was carried out using isobaric tags relative absolute quantitation labeling-based quantitative proteomic approach. The expression macrophage...
Chewing tobacco is a common practice in certain socio-economic sections of southern Asia, particularly the Indian subcontinent and has been well associated with head neck squamous cell carcinoma. The molecular mechanisms chewing which leads to malignancy remains unclear. In large majority studies, short-term exposure evaluated. From biological perspective, however, long-term (chronic) mimics pathogenesis oral cancer more closely. We developed line model investigate chronic effects tobacco....
We have previously identified a putative tumor suppressor gene, NISCH, whose promoter is methylated in lung tissue as well plasma obtained from cancer patients. NISCH was observed to be more frequently smoker patients than non-smoker Here, we investigated the effect of tobacco smoke exposure on methylation gene. tested after oral keratinocytes were exposed mainstream and side stream cigarette extract culture. Methylation region gene also evaluated lifetime non-smokers light smokers (< 20...