A5075531927- Epigenetics and DNA Methylation
- HIV Research and Treatment
- Genetic factors in colorectal cancer
- HIV/AIDS drug development and treatment
- Colorectal Cancer Screening and Detection
- RNA modifications and cancer
- Nuclear Structure and Function
- Cancer Genomics and Diagnostics
- Prostate Cancer Treatment and Research
- HIV/AIDS Research and Interventions
- Cancer-related gene regulation
- Enzyme Structure and Function
- Metastasis and carcinoma case studies
- Cancer-related molecular mechanisms research
- T-cell and Retrovirus Studies
- Prostate Cancer Diagnosis and Treatment
- Gastric Cancer Management and Outcomes
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Cancer, Lipids, and Metabolism
- Protein Structure and Dynamics
- Ferroptosis and cancer prognosis
- Iron Metabolism and Disorders
- Islamic Finance and Communication
- Congenital Diaphragmatic Hernia Studies
OncoMed (United States)
2012-2023
Ghent University
2017
MDxHealth (Netherlands)
2011-2017
Spaarne Gasthuis
2017
Novartis (Switzerland)
2013
Maastricht University
2009-2011
Amsterdam UMC Location Vrije Universiteit Amsterdam
2009
Sidney Kimmel Comprehensive Cancer Center
2009
University Medical Center
2009
University of Colorado Health
2009
The genetic diversity of human immunodeficiency virus (HIV) is a major concern thought to impact on immunologic escape and eventual vaccine efficacy. Here, simple rapid methods are described for the detection estimation divergence between HIV strains basis observation that DNA heteroduplexes formed related sequences have reduced mobility in polyacrylamide gels proportional their degree divergence. Reliable phylogenetic subtypes were assigned HIV-1 from around world. Relationships viruses...
Objective To determine the extent of genetic variation among internationally collected HIV-1 isolates, to analyse phylogenetic relationships and geographic distribution different variants. Design Phylogenetic comparison 70 isolates in 15 countries on four continents. Methods sequence complete gag genome build multiple alignments construct trees using distance matrix methods maximum parsimony algorithms. Results tree analysis identified seven distinct genotypes. The genotypes were evident by...
Upon prolonged treatment with various antiretroviral nucleoside analogs such as 3'-azido-3'-deoxythymidine, 2',3'-dideoxyinosine, 2',3'-dideoxycytidine, (-)- beta-L-2', 3'dideoxy-3'thiacytidine and 2',3'-didehydro-3'-deoxythymidine, selection of human immunodeficiency virus type 1 (HIV-1) strains mutations in the reverse transcriptase (RT) gene has been reported. We designed a hybridization line probe assay (LiPA) for rapid simultaneous characterization following variations RT gene: M41 or...
We have used a gene expression array-based strategy to identify the methylation of tissue factor pathway inhibitor 2 (TFPI2), potential tumor suppressor gene, as frequent event in human colorectal cancers (CRC). TFPI2 belongs recently described group embryonic cell Polycomb (PcG)-marked genes that may be predisposed aberrant DNA early stages carcinogenesis. Aberrant was detected almost all CRC adenomas (97%, n = 56) and I IV CRCs (99%, 115). further explored biomarker for detection using...
Identification of hypermethylated tumor suppressor genes in body fluids is an appealing strategy for the noninvasive detection colorectal cancer. Here we examined role N-Myc downstream-regulated gene 4 (NDRG4) as a novel and biomarker cancer.NDRG4 promoter methylation was analyzed human cancer cell lines, tissue, noncancerous colon mucosa by using methylation-specific polymerase chain reaction (PCR) bisulfite sequencing. NDRG4 mRNA protein expression were studied real-time-PCR...
The genetic diversity of the envelope glycoprotein human immunodeficiency virus type 1 (HIV-1) isolates was studied. HIV-1 were obtained from eight countries in Africa: Djibouti, Gabon, Kenya, Senegal, Somalia, Uganda, Zaire, and Zambia. DNA sequences encoding complete protein PCR amplified sequenced. Phylogenetic relationships among 21 this study 32 previously published full-length env determined. Twenty newly sequenced African could be assigned to subtypes A, C, D, G. One isolate,...
Abstract Purpose: The transcription factors GATA4 and GATA5 are involved in gastrointestinal development inactivated by promoter hypermethylation colorectal cancer. Here, we evaluated GATA4/5 methylation as potential biomarkers for noninvasive cancer detection, investigated the role of Experimental Design: Promoter was analyzed tissue fecal DNA from patients healthy controls using methylation-specific PCR. function tumor suppressors studied inducing overexpression human cell lines. Results:...
Journal Article Two Antigenically Distinct Subtypes of Human Immunodeficiency Virus Type 1: Viral Genotype Predicts Neutralization Serotype Get access John R. Mascola, Mascola Division Retrovirology, Walter Reed Army Institute Research, Henry M. Jackson Foundation for the Advancement Military Medicine, and SRA Laboratories, Inc., Rockville, National Naval Medical Center, Bethesda, Maryland Reprints or correspondence: Dr. 13 Taft Court, Suite 20 L MD 20850. Search other works by this author...
The spread of the human immunodeficiency virus type 1 (HIV-1) is by now virtually worldwide. An understanding genetic, biological, and immunological differences among isolates collected in different geographic locales crucial for development globally effective vaccines. Here we report genetic characteristics 21 HIV-1 from Brazil. were initially characterized using a heteroduplex mobility assay. majority (17 21) related to North American/European reference subtype B. Four belonged more...
In addition to genetic changes, the occurrence of epigenetic alterations is associated with accumulation both and events that promote development progression human cancer. Previously, we reported a set candidate genes comprise part emerging "cancer methylome". present study, first tested 23 for promoter methylation in small number primary colon tumor tissues controls. Based on these results, then examined frequency Oncostatin M receptor-beta (OSMR) larger tissue stool DNA samples collected...
The fecal immunochemical test (FIT) for detecting hemoglobin is used widely noninvasive colorectal cancer (CRC) screening, but its sensitivity leaves room improvement.To identify novel protein biomarkers in stool that outperform or complement CRC and advanced adenomas.Case-control study.Colonoscopy-controlled referral population from several centers.315 samples one series of 12 patients with 10 persons without neoplasia (control samples) a second 81 CRC, 40 adenomas, 43 nonadvanced as well...
Summary: For 4 years, we determined the mode and risk of mother-to-child transmission HTLV-I in a prospective cohort 34 children born to seropositive mothers Franceville, Gabon. We also prevalence antibodies HTLV-I/II siblings mothers. Antibodies were detected by Western blot, proviral DNA was polymerase chain reaction (PCR). The seroconversion anti-HTLV-I for years follow-up 17.5%. Anti-HTLV-I/II only after age 18 months. observed seroprevalence rate 15% among Furthermore, report case...
Using a bioinformatics-based strategy, we set out to identify hypermethylated genes that could serve as biomarkers for early detection of colorectal cancer (CRC) in stool. In addition, the complementary value Fecal Immunochemical Test (FIT) was evaluated. Candidate were selected by applying cluster alignment and computational analysis promoter regions microarray-expression data adenomas carcinomas. DNA methylation measured quantitative methylation-specific PCR on 34 normal colon mucosa, 71...
To identify HIV-1 envelope sequence subtypes in infected individuals from the Russian Federation and Belarus.A cohort of children after exposure to non-sterile needles during 1988-1989 epidemic southern Russia (n = 20) HIV-1-seropositive 1) Belarus 7) via sexual transmission.DNA samples derived peripheral blood mononuclear cells were analysed for their genotypes by heteroduplex mobility assay (HMA). The 1.3 kilobase-pair env gene fragments encoding a portion gp120 amplified nested polymerase...
A cluster sampling survey was performed in 1989 Libreville, Gabon, to determine HTLV-I and HTLV-II prevalence compare the efficacy of polymerase chain reaction (PCR) serology detecting infections. total 322 sera from adults were tested by ELISA Western blot (WB). The WB patterns interpreted according WHO criteria those manufacturer. PCR analysis using primer pairs gag pol region, with a specific probe for HTLV-II, on lymphocytes adults. In addition, 134/322 samples re-tested tax primers,...
Abstract The goal of this retrospective, multicenter study was to evaluate the ability a newly developed refinement quantitative methylation-specific PCR assay detect prostate cancer in histopathologically negative biopsy samples collected from men who were later positively diagnosed during follow-up procedure. Biomarkers tested included much-studied glutathione-S-transferase P1 gene and others reported be frequently methylated cancer. Core tissue subjects with serial biopsies served as...
Colitis-associated colorectal cancer affects individuals with inflammatory bowel disease (IBD) more often and earlier than in the general population. Colonoscopy provides surveillance gold standard. Changes to intervals depending on endoscopic activity have been made, given data demonstrating that this is an important predictor of future dysplasia or cancer, but adjuvant, noninvasive clinical tools are still warranted improve outcomes assist management interpretation dysplasia. Methylation...
Identifying biomarkers in body fluids may improve the noninvasive detection of colorectal cancer. Previously, we identified N-Myc downstream-regulated gene 4 (NDRG4) and GATA binding protein 5 (GATA5) methylation as promising for cancer stool DNA. Here, examined utility NDRG4, GATA5, two additional markers [Forkhead box E1 (FOXE1) spectrin repeat containing nuclear envelope 1 (SYNE1)] promoter plasma Quantitative methylation-specific PCR was performed on DNA from 220 patients with 684...
ABSTRACT The present study evaluated a new confirmatory assay for antibodies to human T-cell lymphotropic virus type 1 and 2 (HTLV-1 HTLV-2) proteins performed with serum samples from various commercial sources. test is line immunoassay (LIA) nylon membrane sensitized the most relevant antigens of HTLVs: envelope gp46 gp21 as well gag p24 p19 antigens, represented by either recombinant or synthetic peptides. A total 176 plasma were tested, which 66 HTLV-1 positive, 72 HTLV-2 38 HTLV...