A5082379064- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Global Cancer Incidence and Screening
- Cancer Risks and Factors
- Histone Deacetylase Inhibitors Research
- Esophageal Cancer Research and Treatment
- Health, Environment, Cognitive Aging
- BRCA gene mutations in cancer
- Fibroblast Growth Factor Research
- Nutrition, Genetics, and Disease
- Cancer-related Molecular Pathways
- Breast Cancer Treatment Studies
- Cardiovascular Health and Risk Factors
- Genetic factors in colorectal cancer
- Nutritional Studies and Diet
- Pesticide and Herbicide Environmental Studies
- Alcohol Consumption and Health Effects
- Folate and B Vitamins Research
- Digital Radiography and Breast Imaging
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- Genomic variations and chromosomal abnormalities
- Weed Control and Herbicide Applications
- Metastasis and carcinoma case studies
University of California, Irvine
2015-2025
UC Irvine Health
2022-2024
Johns Hopkins University
2004-2023
University of California System
2023
Cancer Research Institute
2008-2023
International University of the Caribbean
2020
Biomedical Research Institute
2013
Los Angeles Medical Center
2013
The Barbara Ann Karmanos Cancer Institute
2013
Fred Hutch Cancer Center
2013
Abstract DNA methylation has a role in mediating epigenetic silencing of CpG island genes cancer and other diseases. Identification all gene promoters methylated cells “the methylome” would greatly advance our understanding regulatory networks tumorigenesis. We previously described new method identifying tumor suppressor based on pharmacologic unmasking the promoter region detection re-expression microarray analysis. In this study, we modified improved selection candidates structure...
Objective The aim of this study was to determine whether a dietary intervention designed reduce fat intake and increase fruit, vegetables, whole grains, weight loss, reduces vasomotor symptoms (VMS; ie, hot flashes or night sweats) in postmenopausal women. Methods We included 17,473 US women, ages 50 79 years, at baseline who participated the Women's Health Initiative Dietary Modification trial were not taking menopausal hormone therapy. Logistic regression used evaluate associations....
The human cysteine dioxygenase 1 (CDO1) gene is a non-heme structured, iron-containing metalloenzyme involved in the conversion of to sulfinate, and plays key role taurine biosynthesis. In our search for novel methylated promoters, we have analyzed differential RNA expression profiles colorectal cancer (CRC) cell lines with or without treatment 5-aza-2′-deoxycytidine. Among genes identified, CDO1 promoter was found be differentially primary CRC tissues high frequency compared normal colon...
p63 is a member of the p53 tumor suppressor gene family, which regulates downstream target expression by binding to sequence-specific response elements similar those p53. By using oligonucleotide microarray analysis and analyzing promoters p63-induced genes, we have identified novel p63-specific (p63-REs) in promoter regions EVPL SMARCD3. These p63-REs exhibit characteristic differences from canonical p53-RE (RRRCWWGYYY) both core-binding element (CWWG) as well RRR and/or YYY stretches....
Abstract PGP9.5/UCHL1 is a member of the carboxyl-terminal ubiquitin hydrolase family with potential role in carcinogenesis. We previously identified PGP9.5 as putative tumor-suppressor gene and methylation promoter cancer-specific event primary cancer tissues. In this current study, we analyzed 50 esophageal squamous cell carcinoma (ESCC) tumors well characterized clinicopathologic variables including patient outcome. Two independent modalities for analysis (TaqMan methylation-specific PCR...
Abstract Promoter hypermethylation accompanied by gene silencing is a common feature of human cancers. We identified previously several new tumor suppressor genes based on pharmacologic unmasking the promoter region and detection reexpression microarray analysis. In this study, we modified selection candidates from our previous data excluding that showed basal expression in cancer cell lines. With method, found novel methylated with 90% accuracy. Among these 33 esophageal squamous carcinoma...
Analysis of the length circulating DNA in plasma has been reported as a marker for solid tumor detection. We assessed sensitivity and specificity increased to identify patients with head neck squamous cell carcinoma (HNSCC) monitor posttreatment disease status. Fifty-eight HNSCC paired pre- postoperative 47 samples from control subjects were analyzed using quantitative PCR determine integrity index. found that mean index was significantly greater patients, 0.24 (95% CI: 0.11, 0.38), when...
In addition to genetic changes, the occurrence of epigenetic alterations is associated with accumulation both and events that promote development progression human cancer. Previously, we reported a set candidate genes comprise part emerging "cancer methylome". present study, first tested 23 for promoter methylation in small number primary colon tumor tissues controls. Based on these results, then examined frequency Oncostatin M receptor-beta (OSMR) larger tissue stool DNA samples collected...
Aberrant promoter hypermethylation of several known or putative tumor suppressor genes occurs frequently during the pathogenesis various cancers including breast cancer. Many epigenetically inactivated involved in cancer development remain to be identified. Therefore, this study we used a pharmacologic unmasking approach cell lines with 5-aza-2'-deoxycytidine (5-aza-dC) followed by microarray expression analysis identify cancer.Breast were treated 5-aza-dC We then bisulfite DNA sequencing,...
Background: Glyphosate is the most commonly used herbicide in world and purported to have a variety of health effects, including endocrine disruption an elevated risk several types cancer. Blood DNA methylation has been shown be associated with many other environmental exposures, but our knowledge, no studies date examined association between blood glyphosate exposure. Objective: We conducted epigenome-wide study identify loci urinary its metabolite aminomethylphosphonic acid (AMPA) levels....
Abstract Background: Alcoholic beverages and the main metabolite of alcohol, acetaldehyde, are known carcinogens. A genetic variant in aldehyde dehydrogenase 2 (ALDH2, G>A, rs671) leads to decreased efficiency metabolizing acetaldehyde is associated with increased cancer risk. Since alcohol consumption a modifiable risk factor for various cancers, identification ALDH2 deficiency presents an opportunity precision prevention. Methods: Our primary objectives were examine prevalences...
Diffuse-type gastric cancer (DGC) is the most deadly form of and frequently accompanied by peritoneal dissemination metastasis. The specific molecular events involved in DGC pathogenesis remain elusive. Accumulating evidence epigenetic inactivation tumor suppressor genes led us to conduct a comprehensive screen identify novel methylated human cancers using pharmacologic unmasking subsequent microarray analysis. We compared differential RNA expression profiles intestinal-type (IGC) cell lines...
NMDA receptor Type 2B (NMDAR2B) is a candidate TSG first identified in esophageal squamous cell carcinoma (ESCC). To evaluate NMDAR2B methylation gastric cancer progression, we performed quantitative methylation-specific PCR (MSP), RT-PCR and immnunohistochemistry (IHC) primary tissues colony formation assays lines. We found that the expression of was reactivated by demethylating agent, 5-aza-2'-deoxycytidine, with or without trichostatin A Moreover, inactivation to be closely correlated...
Statins are a class of cholesterol-lowering drugs that affect many intracellular pathways may have implications for chemoprevention against cancer. Epidemiologic data on statins and breast cancer conflicting. We analyzed updated from the Women's Health Initiative (WHI) to assess relationship between risk.
Abstract Promoter DNA hypermethylation with gene silencing is a common feature of human cancer, and cancer-prone methylation believed to be landmark tumor suppressor genes (TSG). Identification novel methylated would not only aid in the development markers but also elucidate biological behavior cancers. We identified several epigenetically silenced candidate TSGs by pharmacologic unmasking esophageal squamous cell carcinoma (ESCC) lines demethylating agents (5-aza-2′-deoxycitidine...
Environmental and lifestyle factors are believed to account for >80% of breast cancers; however, it is not well understood how when these affect risk which exposed individuals will actually develop the disease. While alcohol consumption, obesity, hormone therapy some known cancer, other exposures associated with cancer have yet been identified or characterized. In this paper, proposed that identification blood epigenetic markers personal, in utero, ancestral environmental can help...
p63 mutations have been identified in several developmental abnormalities, including splithand/foot malformation (SHFM). In this study, we demonstrate that the C-terminal domain of p63αassociates with E2 ubiquitin conjugating enzyme, Ubc9. A p63α mutation, Q634X, whichnaturally occurs SHFM modulated interaction Ubc9 yeast genetic assay.Furthermore, catalyzed conjugation small modifier-1 (SUMO-1),which covalently modified vitro and vivo at two positions (K549E K637E), eachsituated...
Abstract Deleted in Colorectal Cancer (DCC) is a putative tumor suppressor gene, whose loss has been implicated colorectal tumorigenesis. Decreased or of DCC expression demonstrated number human cancers, including esophageal cancer. In this study, we analyzed squamous cell carcinoma (ESCC) lines and primary ESCCs as well normal tissues for methylation by bisulfite sequencing, methylation‐specific PCR (MSP) and/or quantitative (qMSP). When qMSP cut‐off value positivity was set to 1.0,...
The ability of tamoxifen and raloxifene to decrease breast cancer risk varies among different subtypes. It is important determine one’s subtype-specific when considering chemoprevention. A number single nucleotide polymorphisms (SNPs), including one in caspase-8 (CASP8), have been previously associated with developing cancer. Because an protein involved receptor-mediated apoptosis whose activity affected by estrogen, we hypothesized that additional SNPs CASP8 could be risk, perhaps a manner....
There is strong evidence that leisure-time physical activity protective against postmenopausal breast cancer risk but the association with premenopausal less clear. The purpose of this study was to examine developing cancer.