A5081464841- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Virology and Viral Diseases
- Respiratory viral infections research
- T-cell and Retrovirus Studies
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Reproductive tract infections research
- Synthesis and Biological Evaluation
- Vector-Borne Animal Diseases
- Animal Disease Management and Epidemiology
- Parasites and Host Interactions
- Syphilis Diagnosis and Treatment
- Bacteriophages and microbial interactions
- vaccines and immunoinformatics approaches
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Immunodeficiency and Autoimmune Disorders
- Sex work and related issues
- Microbial infections and disease research
- Mosquito-borne diseases and control
- COVID-19 epidemiological studies
- Plant Virus Research Studies
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
Hospices Civils de Lyon
2013-2015
Université Claude Bernard Lyon 1
2013
Fujirebio (Belgium)
1998-2003
Fundação Oswaldo Cruz
1999
Hospital Universitário Clementino Fraga Filho
1999
Universidade Federal do Rio de Janeiro
1999
Czech Academy of Sciences, Institute of Microbiology
1999
Universidade Federal do Piauí
1999
University of British Columbia
1992-1996
Diversité, adaptation et développement des plantes
1995
ISGlobal work is supported by the Departament d’Universitats i Recerca de la Generalitat Catalunya, Spain (AGAUR; 017SGR00924) and Instituto Salud Carlos III (ISCIII) RICET Network for Cooperative Research in Tropical Diseases (ISCIII; RD16/0027/0004 - PI1290) FEDER. MJP research Ministry of Health, Government Catalonia (PERIS 2016-2010 SLT008/18/00132). ICA, JG, FT are grant number U01AI129783 from National Institute Allergy Infectious (NIAID), Institutes Health (NIH). ICA also partly...
Background Chagas disease is due to the parasite Trypanosoma cruzi, a protist disseminated by Triatome vector. This endemic Latin America and considered WHO as one of 17 world's neglected diseases. In Europe in North America, imported cases are also detected, migration population outside region. Diagnosis T. cruzi infection usually made indirectly detection specific antibodies antigens. Following initial diagnostic evaluation or screening test (qualifying discarding blood donation),...
Residents of nursing homes (NHs) are at high risk coronavirus disease 2019 (COVID-19)-related and death may respond poorly to vaccination because old age frequent comorbid conditions.Seventy-eight residents 106 staff members, naive infection or previously infected with severe acute respiratory syndrome (SARS-CoV-2), were recruited in NHs Belgium before immunization 2 doses 30 µg BNT162b2 messenger RNA (mRNA) vaccine days 0 21. Binding antibodies (Abs) SARS-CoV-2 receptor-binding domain...
Rubella virus (RV)-specific immunoglobulin G antibodies were studied by enzyme-linked immunosorbent assay (ELISA) techniques in sera from RV (RA 27/3)-vaccinated individuals, patients experiencing natural infection, congenital rubella syndrome patients, and individuals failing to respond repeated immunization. Results obtained using whole-RV ELISAs (detergent-solubilized M33 strain or intact Gilchrist strain) hemagglutination inhibition (HAI) neutralization (NT) assays compared with results...
Trypanosoma cruzi parasite, the causative agent of Chagas disease, infects about six million individuals in more than 20 countries. Monitoring parasite persistence infected is utmost importance to develop and evaluate treatments control disease. Routine screening for human achieved by serological assays; PCR testing monitor spontaneous or therapy-induced parasitological cure has limitations due low fluctuating parasitic load circulating blood. The aim present study a newly developed antibody...
ABSTRACT We evaluated the sensitivity and specificity of a new confirmatory test for treponemal antibodies, INNO-LIA Syphilis (Innogenetics NV, Ghent, Belgium), on large number sera from clinical laboratory. This multiparameter line immunoassay (LIA) uses recombinant synthetic polypeptide antigens derived Treponema pallidum proteins. In single-blinded cross-sectional retrospective study, 289 seronegative sera, 219 seropositive 23 with an indeterminate serological status syphilis were...
ABSTRACT The commercially available diagnostic tests for syphilis are mostly based on the use of extracted antigens Treponema pallidum . Pronounced cross-reactivities with other spirochete often reported. aim this study was to validate a novel multiparametric assay (the performed kit) INNO-LIA Syphilis confirmation antibodies in set 840 documented human serum samples. All samples were previously tested at French World Health Organization reference center venereal diseases (Institute Alfred...
ABSTRACT We have evaluated a new serological confirmatory test (INNO-LIA HTLV I/II Ab [INNO-LIA]) for human T-cell leukemia virus (HTLV) using large collection of samples from Brazilian blood donors (São Paulo region) and compared the results with those obtained by Western blotting (WB) tests (WB2.3 WB2.4). Blood donations were initially screened enzyme-linked immunosorbent assays (ELISAs) based on viral lysates, repeatedly reactive further tested WB2.3. When available, also PCR, two...
ABSTRACT Although screening for Trypanosoma cruzi antibodies is mandatory in most South American countries, current tests are insensitive and have poor specificity. A recently optimized line immunoassay (the INNO-LIA Chagas assay) the serological confirmation of Chagas' disease was evaluated at a large blood bank São Paulo, Brazil. Sera from donors who reacted least one three assays ( n = 1,604) returned follow-up were retested, interviewed to assess their epidemiological risk. The results...
This work reports a comparison of an enzyme immunoassay (EIA) using two major Treponema pallidum recombinant antigens with T. hemagglutination (TPHA) assay and nontreponemal Venereal Disease Reference Laboratory (VDRL) test. A total 1,822 normal donor serum samples was tested for cardiolipin antibodies, respectively, by the VDRL EIA. Among these samples, 440 were further TPHA technology. Four found positive EIA, while all reported to be negative both routine assays. Subsequent testing...
Background Chagas disease is caused by the protozoan parasite Trypanosoma cruzi and a serious public health problem throughout Latin America. With 6 million people infected, there major international effort to develop new drugs. In chronic phase of disease, burden extremely low, infections are highly focal at tissue/organ level, bloodstream parasites only intermittently detectable. As result, clinical trials constrained difficulties associated with determining parasitological cure. Even...
Abstract Background Residents of nursing homes (NH) are at high risk COVID-19 related morbidity and death may respond poorly to vaccination because old age frequent comorbidities. Methods Forty residents forty staff members either naïve or previously infected with SARS-CoV-2 were recruited in two NH Belgium before immunization doses 30µg BNT162b2 mRNA vaccine day 0 21. Binding antibodies (Ab) receptor binding domain (RBD), spike domains S1 S2, RBD Ab avidity, neutralizing against wild type...
BACKGROUND: The transfusion of contaminated blood has become the major route transmission for Chagas' disease in Brazil. Current screening tests are insensitive and yield conflicting results, while confirmatory assays do not exist. A line immunoassay (INNO‐LIA Chagas Ab [INNO‐LIA]) combining relevant, immunodominant recombinant synthetic antigens on a single nylon membrane strip was evaluated serologic confirmation disease. STUDY DESIGN AND METHODS: Sera from 1062 patients healthy residents...
Cross-reactive antibodies are characterized by their recognition of antigens that different from the trigger immunogen. This happens when similarity between two antigenic determinants becomes adequate enough to enable a specific binding with such cross-reactive antibodies. In present manuscript, we report presence, at an "abnormal" high frequency, in blood samples French human subjects cross-reacting synthetic-peptide antigen derived Trypanosoma cruzi (T. cruzi) protein sequence. As vector...
ABSTRACT The present study evaluated a new confirmatory assay for antibodies to human T-cell lymphotropic virus type 1 and 2 (HTLV-1 HTLV-2) proteins performed with serum samples from various commercial sources. test is line immunoassay (LIA) nylon membrane sensitized the most relevant antigens of HTLVs: envelope gp46 gp21 as well gag p24 p19 antigens, represented by either recombinant or synthetic peptides. A total 176 plasma were tested, which 66 HTLV-1 positive, 72 HTLV-2 38 HTLV...
Rubella virus (RV)-specific immunoglobulin G (IgG) antibodies were studied in military recruits undergoing unselected immunization with live attenuated measles, mumps, and rubella (MMR) vaccine. Three different whole-RV enzyme immunoassays (EIAs) an epitope-specific EIA a synthetic peptide (BCH-178c) representing heutralization domain on the RV E1 envelope protein used. Before vaccination, 84.2, 87.7, 84.5% of subjects tested (n = 399) found to be seropositive (> 10 IU/ml or assay...
Chagas disease, caused by the protozoan Trypanosoma cruzi, affects more than 6 million people worldwide. Following a mostly asymptomatic acute phase, disease progresses to long-lasting chronic phase throughout which life-threatening disorders heart and/or gastrointestinal tract will manifest in about 30% of those chronically infected. During parasitemia is low and intermittent, while high level anti-T. cruzi antibodies persist for years. These two features hamper post-chemotherapeutic...
A total of 250 human serum samples were tested for rubella virus immunoglobulin G antibodies by two enzyme immunoassays (EIAs), one using whole antigen and the other based on use synthetic peptide antigen. The taken from 125 volunteers before after their immunization with RA 27/3 vaccine. This study indicates that a peptide-based EIA can favorably replace current viral lysate-based EIAs to detect following immunization. Because used in this newly developed represents putative neutralization...
Currently, serodiagnosis of infection with the helminth parasite Onchocerca volvulus is limited to Ov-16 IgG4 test, a test that has sensitivity and suboptimal specificity. In previous studies, we identified several linear epitopes have potential supplement diagnostic toolbox for onchocerciasis.In this study three peptides, bearing in total six were transferred multiplex ELISA platform. This was used assess clinical utility peptide serology markers by analyzing sample sets from both O....
The influence of glutamic acid (E)-alanine (A) dimorphism at position 74 the DR4β chain on cytotoxic T cell recognition an antigenic rubella virus peptide, E1(273–284), was studied using a panel B lines and transfectants expressing different HLA-DRB1 alleles as antigen-presenting cells targets in 51Cr-release assays. Only DRB1*0403, DRB1*0406 or DRB 1*0407 subtypes which shared residue, E, when sensitized with E1(273–284) elicited strong lymphocyte responses. However, direct binding antibody...