A5000674089- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Nail Diseases and Treatments
- Plant Pathogens and Fungal Diseases
- Antimicrobial Peptides and Activities
- Infectious Diseases and Mycology
- T-cell and Retrovirus Studies
- Plant-Microbe Interactions and Immunity
- Phytoplasmas and Hemiptera pathogens
- Yeasts and Rust Fungi Studies
- Parasitic Infections and Diagnostics
- Toxin Mechanisms and Immunotoxins
- Peptidase Inhibition and Analysis
- Science and Education Research
- Cutaneous lymphoproliferative disorders research
- Invertebrate Immune Response Mechanisms
- Toxoplasma gondii Research Studies
- Women's cancer prevention and management
- vaccines and immunoinformatics approaches
- Dermatology and Skin Diseases
- Polysaccharides and Plant Cell Walls
- Immunotherapy and Immune Responses
- Insect symbiosis and bacterial influences
- Pneumonia and Respiratory Infections
- Plant Disease Management Techniques
Universidade de São Paulo
2016-2025
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2009-2024
Universidade Cidade de São Paulo
2022
Instituto de Medicina Tropical
2020
Universidade Federal de São Paulo
1995-2017
Institute of Biomedical Science
2005-2016
Instituto de Medicina Avançada
2014
Instituto Butantan
2013
Hospital de Clínicas
2009
Universidade Brasil
2009
ABSTRACT Superoxide dismutase (SOD) is an enzyme that converts superoxide radicals into hydrogen peroxide and molecular oxygen has been shown to contribute the virulence of many human-pathogenic bacteria through its ability neutralize toxic levels reactive species generated by host. SOD also speculated be important in pathogenesis fungal infections, but role this not rigorously investigated. To examine contribution we cloned Cu,Zn SOD-encoding gene ( SOD1 ) from yeast Cryptococcus neoformans...
Abstract Despite a century of study, the relationship between Ag-specific Ig concentration and protection remains poorly understood for majority pathogens. In certain conditions, administration high Ab doses before challenge with an infectious agent can be less effective than smaller doses, phenomenon which is consistent prozone-like effect. this IgG1, IgG2a, IgG2b, IgG3 dose, infective inocula, was investigated in mouse model Cryptococcus neoformans infection. The activity each IgG subclass...
ABSTRACT The 43-kDa glycoprotein of Paracoccidioides brasiliensis is the major diagnostic antigen paracoccidioidomycosis, prevalent systemic mycosis Latin America. Apart from eliciting high antibody titers, gp43 also immunodominant in delayed-type hypersensitivity reactions infected animals and humans. cellular immune response mice to administered complete Freund’s adjuvant involves CD4 + Th-1 lymphocytes, secreting gamma interferon (IFN-γ) interleukin 2 (IL-2) but not IL-4 IL-10. T-cell...
Macrophages are key players during Paracoccidioides brasiliensis infection. However, the relative contribution of fungal response to counteracting macrophage activity remains poorly understood. In this work, we evaluated P. proteomic internalization. A total 308 differentially expressed proteins were detected in The positively regulated included those involved alternative carbon metabolism, such as enzymes gluconeogenesis, beta-oxidation fatty acids and amino catabolism. down-regulated...
Background Serological tests have long been established as rapid, simple and inexpensive tools for the diagnosis follow-up of PCM. However, different protocols antigen preparations are used few attempts to standardize routine serological methods not succeeded. Methodology/Principal findings We compared performance six Brazilian reference centers Each center provided 30 sera PCM patients, with positive high, intermediate low titers, which were defined "reference" titers. then applied its own...
The ability of Paracoccidioides to defend itself against reactive oxygen species (ROS) produced by host effector cells is a prerequisite survive. To counteract these radicals, expresses, among different antioxidant enzymes, superoxide dismutases (SODs). In this study, we identified six SODs isoforms encoded the genome. We determined gene expression levels representative isolates phylogenetic lineages spp. (S1, PS2, PS3 and Pb01-like) using quantitative RT-PCR. Assays were carried out analyze...
Abstract Complement component 3 (C3) is the major opsonin for pathogenic fungus Cryptococcus neoformans in non‐immune host. However, efficiency of complement‐mediated opsonization varies, depending on strain, through mechanisms that are not understood. Analysis phagocytosis 12 strains grown Sabouraud medium revealed phagocytic indices were inversely correlated with capsule volume. In contrast, there was no correlation between index and volume IgG1‐opsonized cells. When size increased,...
Phenotypic switching has been linked to the virulence of many pathogens, including fungi. However, it not conclusively shown occur in vivo or influence outcome infection. Cryptococcus neoformans undergoes phenotypic vitro colony types that differ their mice. In this study, we asked whether C. and phenomenon contributes virulence. By using a small inoculum preclude introduction variants had already switched during propagation, demonstrated mucoid phenotype occurred two mice strains was...
A PCR assay based on oligonucleotide primers derived from the sequence of gene coding for 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis DNA in sputa. In standardized conditions, it could 10 cells/ml sputum, providing sufficient accuracy be useful diagnosis paracoccidioidomycosis.
Abstract The IgM mAbs 12A1 and 13F1 are protective nonprotective, respectively, against lethal Cryptococcus neoformans infection in mice. To better understand the variables that contribute to efficacy C. neoformans, we studied effects of inoculum size, route infection, Ab dose for each these mAbs. mAb did not prolong survival under any condition studied. prolonged after administration certain doses i.p. with defined inocula promoted phagocytosis, agglutination, formation inflammatory cell...
Paracoccidioidomycosis is the most prevalent systemic endemic mycosis in South America with reported cases Brazil. It a major cause of disability and death among young adult rural workers during their productive years life. Sequels are frequent evolution disease mortality burden strongly influenced by socio-economic status patients. Although long periods antifungal therapy (sulfamethoxazole/trimethoprim, itraconazole amphotericin B) used clinical practice, relapses remain significant...
We show that RsAFP2, a plant defensin interacts with fungal glucosylceramides, is active against Candida albicans, inhibits to lesser extent other species, and nontoxic mammalian cells. Moreover, glucosylceramide levels in species correlate RsAFP2 sensitivity. found prophylactically effective murine candidiasis.
Non-mammalian models have been used to investigate fungal virulence. In this work we explored the use of Galleria mellonella as an infection model for pathogenic dimorphic fungi Histoplasma capsulatum and Paracoccidioides lutzii. mammalian these cause similar infections, disease outcomes are influenced by quantity infective inocula. We describe a aspect in G. characterize pathogenesis features system. Infection with P. lutzii or H. capsulatum, all inoculum used, killed larvae at 25 37°C....
ABSTRACT Vaccination with peptide 10 (P10), derived from the Paracoccidioides brasiliensis glycoprotein 43 (gp43), induces a Th1 response that protects mice in an intratracheal P. infection model. Combining P10 complete Freund's adjuvant (CFA) or other adjuvants further increases peptide's antifungal effect. Since dendritic cells (DCs) are up to 1,000-fold more efficient at activating T than CFA, we examined impact of P10-primed bone-marrow-derived DC vaccination mice. Splenocytes immunized...
Commensal yeast from the genus Candida is part of healthy human microbiota. In some cases, spp. dysbiosis can result in candidiasis, symptoms which may vary mild localized rashes to severe disseminated infections. The most prevalent treatments against candidiasis involve fluconazole, itraconazole, miconazole, and caspofungin. Moreover, amphotericin B associated with prolonged azole administration utilized control cases. Currently, numerous guidelines recommend echinocandins treat invasive...