A5101932528- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Nail Diseases and Treatments
- Plant Pathogens and Fungal Diseases
- Yeasts and Rust Fungi Studies
- Infectious Diseases and Mycology
- T-cell and Retrovirus Studies
- Phytoplasmas and Hemiptera pathogens
- Toxin Mechanisms and Immunotoxins
- Plant-Microbe Interactions and Immunity
- Cutaneous lymphoproliferative disorders research
- Plant Disease Resistance and Genetics
- Fungal and yeast genetics research
- Cytomegalovirus and herpesvirus research
- Women's cancer prevention and management
- Pneumocystis jirovecii pneumonia detection and treatment
- Neutropenia and Cancer Infections
- Mycobacterium research and diagnosis
- Historical Medical Research and Treatments
- Cancer Treatment and Pharmacology
- Infections and bacterial resistance
- Parasitic Infections and Diagnostics
- Empathy and Medical Education
- HIV/AIDS oral health manifestations
- Fungal Biology and Applications
North Mississippi Medical Center
2024
Fundación Santa Fe de Bogotá
2024
Bosque School
2024
Corporación para Investigaciones Biológicas
2009-2023
Universidad de Antioquia
1974-2023
Universidad del Rosario
2015
University of Toronto
2010
Hospital for Sick Children
2010
SickKids Foundation
2010
Kangwon National University
2010
Paracoccidioides brasiliensis is the etiologic agent of paracoccidioidomycosis, a disease confined to Latin America and marked importance in endemic areas due its frequency severity. This species considered be clonal according mycological criteria has been shown vary virulence. To characterize natural genetic variation reproductive mode this fungus, we analyzed P. phylogenetically search cryptic possible recombination using concordance nondiscordance gene genealogies with respect phylogenies...
Evidence that disease due to the thermally dimorphic fungus Paracoccidioides brasiliensis occurs post-puberty predominantly in males led us hypothesize hormonal factors critically affect its pathogenesis. We show here estrogens inhibit mycelial- yeast-form transformation of P. vitro. Transformation three isolates was inhibited 71, 33, and 19% control values presence 10(-10), 10(-8), 10(-6) M 17 beta-estradiol, respectively. The synthetic estrogen diethylstilbestrol active but less potent...
Paracoccidioidomycosis, a disease caused by Paracoccidioides brasiliensis, which is endemic to Latin America, much more common in men than women, suggesting role for hormonal factors. We recently showed that two other yeasts possess steroid binding proteins and postulated these receptor-like molecules represented mechanism the milieu of host might influence an infecting pathogen. Therefore, we examined P. brasiliensis sex protein. Because tritiated steroids rapidly dissociated from fungal...
We describe the case of a diabetic patient who developed severe invasive fungal infection due to Rhizopus species postoperatively after dual heart/kidney transplantation with subsequent intensive immunosuppressive therapy. No improvement was noted amphotericin B (deoxycholate) therapy, but salvage treatment new azole antifungal posaconazole (200 mg orally 4 times daily) resulted in dramatic clinical as early 1 week initiation therapy that continued through 23 weeks treatment, marked...
Eumycetoma and chromoblastomycosis are chronic, disfiguring fungal infections of the subcutaneous tissue that rarely resolve spontaneously. Most patients do not achieve sustained long-term benefits from available treatments; therefore, new therapeutic options needed. We evaluated efficacy posaconazole, a extended-spectrum triazole antifungal agent, in 12 with eumycetoma or refractory to existing therapies. Posaconazole 800 mg/d was given divided doses for maximum 34 months. Complete partial...
We studied 52 patients with disseminated histoplasmosis, 30 the acquired immunodeficiency syndrome (AIDS) (cohort 1) and 22 not co-infected human virus 2). Demographic, clinical, laboratory, mycologic findings, as well antifungal therapy highly active antiretroviral (HAART), were analyzed. Skin lesions significantly higher in cohort 1 than 2 (P = 0.001). Anemia, leukopenia, an elevated erythrocyte sedimentation rate also more pronounced < Histoplasma capsulatum was isolated often 0.05)...
The fungicidal capacity of murine pulmonary macrophages (PuM) activated in vitro with IFN or lymphokines vivo was studied. PuM treated overnight (1000 U/ml), Con A-stimulated spleen cell culture supernatants, lymph node cells plus A significantly killed yeast the Gar w isolate Paracoccidioides brasiliensis 45.5 +/- 2.1%, 72.0 4.2%, and 51.5 0.7% respectively. Two other isolates P. (Ru LA) were also (45 34%) by A. Control had lesser but significant for killing isolates, ranging from 15 to...
The effect of coculturing yeast-form Paracoccidioides brasiliensis with murine cells was studied. Coculture resident peritoneal or pulmonary macrophages P. for 72 h dramatically enhanced fungal multiplication 19.3 +/- 2.4- and 4.7 0.8-fold, respectively, compared cocultures lymph node complete tissue culture medium alone. Support by macrophage dose dependent. Lysates macrophages, supernatants from cultures, McVeigh-Morton broth, like medium, did not support in 72-h cultures. Time course...
The ability of Paracoccidioides to defend itself against reactive oxygen species (ROS) produced by host effector cells is a prerequisite survive. To counteract these radicals, expresses, among different antioxidant enzymes, superoxide dismutases (SODs). In this study, we identified six SODs isoforms encoded the genome. We determined gene expression levels representative isolates phylogenetic lineages spp. (S1, PS2, PS3 and Pb01-like) using quantitative RT-PCR. Assays were carried out analyze...
ABSTRACT Melanin is made by several important pathogenic fungi and has been implicated in the pathogenesis of a number fungal infections. This study investigated whether thermally dimorphic pathogen Histoplasma capsulatum var . produced melanin or melanin-like compounds vitro during infection. Growth H. mycelia chemically defined minimal medium pigmented conidia. yeast with l -3,4-dihydroxyphenylalanine (DOPA) (-)-epinephrine cells. Treatment cells proteolytic enzymes, denaturant, hot...
Most of our knowledge concerning the virulence determinants pathogenic fungi comes from infected host, mainly animal models and more recently in vitro studies with cell cultures. The usually present intra- and/or extracellular host-parasite interfaces, parasitism phenomenon dependent on complementary surface molecules. Among living organisms, this has been characterized as a cohabitation event, where fungus is able to recognize specific host tissues acting an attractant, creating stable...
ABSTRACT Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, an important human systemic mycosis in Latin America. Recently, existence three different phylogenetic species (S1, PS2, and PS3) P. was demonstrated. Despite being genetically isolated, all were capable inducing disease both humans animals, although lower virulence has been found with PS2 species. The available molecular methods developed to characterize type strains have not useful for assigning...
Glycoprotein gp43 is an immunodominant diagnostic antigen for paracoccidioidomycosis caused by Paracoccidioides brasiliensis. It abundantly secreted in isolates such as Pb339. structurally related to beta-1,3-exoglucanases, however inactive. Its function fungal biology unknown, but it elicits humoral, innate and protective cellular immune responses; binds extracellular matrix-associated proteins. In this study we applied antisense RNA (aRNA) technology Agrobacterium tumefaciens-mediated...
Itraconazole effectively controls active paracoccidioidomycosis but appears not to hinder lung fibrosis. Clinical records and chest radiographs from 47 itraconazole-treated patients with prolonged posttherapy follow-up (mean period, 5.6 years) were analyzed; the interpreted following pneumoconiosis standards that consider lungs as 6 fields grade damage according number of involved. Infiltrative lesions observed at diagnosis in 93.6% patients. Fibrosis was 31.8% had cleared end observation...
Background. The incidence of invasive fungal infections (IFIs) in solid organ transplant (SOT) recipients has increased during the past 20 years and is associated with significant morbidity mortality. In this post hoc analysis a large, open-label, multicenter study, we evaluated efficacy safety posaconazole, new extended-spectrum triazole, as salvage therapy for IFIs SOT recipients. Methods. Twenty-three proven or probable IFI evidence disease refractory to, intolerant of, standard...
ABSTRACT One of the most crucial events during infection with dimorphic fungus Paracoccidioides brasiliensis is adhesion to pulmonary epithelial cells, a pivotal step in establishment disease. In this study, we have evaluated relevance 32-kDa protein, putative member haloacid dehalogenase (HAD) superfamily hydrolases, virulence fungus. Protein sequence analyses supported inclusion PbHad32p as hydrolase and revealed conserved protein only among fungal filamentous pathogens that are closely...