A5078271108- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Antifungal resistance and susceptibility
- Yeasts and Rust Fungi Studies
- Nail Diseases and Treatments
- Plant-Microbe Interactions and Immunity
- Infectious Diseases and Mycology
- Phytoplasmas and Hemiptera pathogens
- T-cell and Retrovirus Studies
- Plant Disease Resistance and Genetics
- Hormonal Regulation and Hypertension
- Mycorrhizal Fungi and Plant Interactions
- Fungal and yeast genetics research
- Genomics and Phylogenetic Studies
- Parasitic Infections and Diagnostics
- Cytomegalovirus and herpesvirus research
- Mycotoxins in Agriculture and Food
- Toxin Mechanisms and Immunotoxins
- Nutrition, Genetics, and Disease
- Studies on Chitinases and Chitosanases
- Reproductive tract infections research
- Cardiovascular and exercise physiology
- Plant Pathogens and Resistance
- Genetic Associations and Epidemiology
- Cardiovascular Health and Disease Prevention
Corporación para Investigaciones Biológicas
2016-2025
Universidad de Antioquia
2016-2025
Universidad del Rosario
2016-2019
IRCCS Istituto Auxologico Italiano
2016
Istituti di Ricovero e Cura a Carattere Scientifico
2016
University of Milano-Bicocca
2016
Institute of Biomedical Science
2005
Universidade de São Paulo
2005
University of Maryland, Baltimore
1992
Weizmann Institute of Science
1988-1992
Paracoccidioides brasiliensis is the etiologic agent of paracoccidioidomycosis, a disease confined to Latin America and marked importance in endemic areas due its frequency severity. This species considered be clonal according mycological criteria has been shown vary virulence. To characterize natural genetic variation reproductive mode this fungus, we analyzed P. phylogenetically search cryptic possible recombination using concordance nondiscordance gene genealogies with respect phylogenies...
The use of molecular taxonomy for identifying recently diverged species has transformed the study speciation in fungi. pathogenic fungus Paracoccidioides spp been hypothesized to be composed five phylogenetic species, four which compose brasiliensis complex. Nuclear gene genealogies support this divergence scenario, but mitochondrial loci do not; while all from complex are differentiated at nuclear coding loci, they not loci. We addressed source incongruity using 11 previously published...
Abstract Until recently, Histoplasma capsulatum was believed to harbour three varieties, var. (chiefly a New World human pathogen), duboisii (an African pathogen) and farciminosum Old horse which varied in clinical manifestations geographical distribution. We analysed the phylogenetic relationships of 137 individuals representing varieties from six continents using DNA sequence variation four independent protein‐coding genes. At least eight clades were idengified: (i) North American class 1...
Journal Article Experimental murine paracoccidiodomycosis induced by the inhalation of conidia Get access Juan G. McEwen, McEwen Search for other works this author on: Oxford Academic PubMed Google Scholar Victor Bedoya, Bedoya Maria M. Patiño, Patiño E. Salazar, Salazar Angela Restrepo Medical and Veterinary Mycology, Volume 25, Issue 3, May 1987, Pages 165–175, https://doi.org/10.1080/02681218780000231 Published: 01 1987 history Accepted: 04 February
Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, dimorphic fungus in order Onygenales, coupled with thermally regulated transition from soil-dwelling filamentous form yeast-like pathogenic form. To better understand genetic basis growth pathogenicity we sequenced genomes two strains brasiliensis (Pb03 Pb18) one strain lutzii (Pb01). These range size 29.1 Mb 32.9...
Recent discoveries of novel systemic fungal pathogens with thermally dimorphic yeast-like phases have challenged the current taxonomy Ajellomycetaceae, a family currently comprising genera Blastomyces, Emmonsia, Emmonsiellopsis, Helicocarpus, Histoplasma, Lacazia and Paracoccidioides. Our morphological, phylogenetic phylogenomic analyses demonstrated species relationships their specific phenotypes, clarified generic boundaries provided first annotated genome assemblies to support description...
Characterization of genetic differences between lineages the dimorphic human-pathogenic fungus Paracoccidioides can identify changes linked to important phenotypes and guide development new diagnostics treatments. In this article, we compared genomes 31 diverse isolates representing major spp. completed first annotated genome sequences for PS3 PS4 lineages. We analyzed population structure characterized diversity among , including a deep split S1 into two (S1a S1b), differentiated S1b,...
Macrophages are key players during Paracoccidioides brasiliensis infection. However, the relative contribution of fungal response to counteracting macrophage activity remains poorly understood. In this work, we evaluated P. proteomic internalization. A total 308 differentially expressed proteins were detected in The positively regulated included those involved alternative carbon metabolism, such as enzymes gluconeogenesis, beta-oxidation fatty acids and amino catabolism. down-regulated...
Three closely related thermally dimorphic pathogens are causal agents of major fungal diseases affecting humans in the Americas: blastomycosis, histoplasmosis and paracoccidioidomycosis. Here we report genome sequence analysis four strains etiological agent Blastomyces, two species genus Emmonsia, typically small mammals. Compared to species, Blastomyces genomes highly expanded, with long, often sharply demarcated tracts low GC-content sequence. These GC-poor isochore-like regions enriched...
The ability of Paracoccidioides to defend itself against reactive oxygen species (ROS) produced by host effector cells is a prerequisite survive. To counteract these radicals, expresses, among different antioxidant enzymes, superoxide dismutases (SODs). In this study, we identified six SODs isoforms encoded the genome. We determined gene expression levels representative isolates phylogenetic lineages spp. (S1, PS2, PS3 and Pb01-like) using quantitative RT-PCR. Assays were carried out analyze...
ABSTRACT Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, an important human systemic mycosis in Latin America. Recently, existence three different phylogenetic species (S1, PS2, and PS3) P. was demonstrated. Despite being genetically isolated, all were capable inducing disease both humans animals, although lower virulence has been found with PS2 species. The available molecular methods developed to characterize type strains have not useful for assigning...
Iron is essential for the proliferation of fungal pathogens during infection. The availability iron limited due to its association with host proteins. Fungal have evolved different mechanisms acquire from host; however, little known regarding how Paracoccidioides species incorporate and metabolize this ion. In work, sources that are used by spp. were investigated. Robust growth in presence iron-containing molecules hemin hemoglobin was observed. present hemolytic activity ability internalize...
Glycoprotein gp43 is an immunodominant diagnostic antigen for paracoccidioidomycosis caused by Paracoccidioides brasiliensis. It abundantly secreted in isolates such as Pb339. structurally related to beta-1,3-exoglucanases, however inactive. Its function fungal biology unknown, but it elicits humoral, innate and protective cellular immune responses; binds extracellular matrix-associated proteins. In this study we applied antisense RNA (aRNA) technology Agrobacterium tumefaciens-mediated...
The genus Aspergillus comprises a diverse group of fungi that can cause range health issues, including systemic infections and allergic reactions. In this regard, A. fumigatus has been recognized as the most prevalent allergen-producing species. This taxonomic classification subject to frequent updates, which generated considerable difficulties for its when traditional identification methodologies are employed. To demonstrate feasibility approach, we sequenced whole genomes 81 isolates...
ABSTRACT One of the most crucial events during infection with dimorphic fungus Paracoccidioides brasiliensis is adhesion to pulmonary epithelial cells, a pivotal step in establishment disease. In this study, we have evaluated relevance 32-kDa protein, putative member haloacid dehalogenase (HAD) superfamily hydrolases, virulence fungus. Protein sequence analyses supported inclusion PbHad32p as hydrolase and revealed conserved protein only among fungal filamentous pathogens that are closely...
Background Paracoccidioides brasiliensis is a human thermal dimorphic pathogenic fungus. Survival of P. inside the host depends on adaptation this fungal pathogen to different conditions, namely oxidative stress imposed by immune cells. Aims and Methodology In study, we evaluated role alternative oxidase (AOX), an enzyme involved in intracellular redox balancing, during host-P. interaction. We generated mitotically stable AOX (PbAOX) antisense RNA (aRNA) strain with 70% reduction gene...
Paracoccidiodomycosis (PCM) is a clinically important fungal disease that can acquire serious systemic forms and caused by the thermodimorphic Paracoccidioides spp. PCM tropical endemic in Latin America, where up to ten million people are infected; 80% of reported cases occur Brazil, followed Colombia Venezuela. To enable genomic studies better characterize pathogenesis this dimorphic fungus, two reference strains P. brasiliensis (Pb03, Pb18) one strain lutzii (Pb01) were sequenced [1]....
The sporulation capacities of the mycelial form Paracoccidioides brasiliensis were determined. Five different culture media used and four human isolates studied. Conidia produced in three agar media, namely water-agar, glucose-salts yeast-extract. Corn meal Sabouraud dextrose agars failed to induce sporulation. Various types spores characterized with peculiar bulging arthroconidia single-celled, pear-shaped conidia predominating. size these varied from 3·6 4·6 μm length. It is concluded that...