Evidence that disease due to the thermally dimorphic fungus Paracoccidioides brasiliensis occurs post-puberty predominantly in males led us hypothesize hormonal factors critically affect its pathogenesis. We show here estrogens inhibit mycelial- yeast-form transformation of P. vitro. Transformation three isolates was inhibited 71, 33, and 19% control values presence 10(-10), 10(-8), 10(-6) M 17 beta-estradiol, respectively. The synthetic estrogen diethylstilbestrol active but less potent...

ABSTRACT We have developed a murine model of pulmonary infection by Paracoccidioides brasiliensis in which resistance was associated with immunological activities governed gamma interferon (IFN-γ). To better characterize this model, we measured type 1 and 2 cytokines the lungs investigated effect endogenous IFN-γ depletion monoclonal antibodies course susceptible (B10.A) resistant (A/Sn) mice. At weeks 4 8 after infection, from animals presented levels IFN-γ, interleukin-4 (IL-4), IL-5,...

ABSTRACT Paracoccidioidomycosis, a systemic mycosis restricted to Latin America and produced by the dimorphic fungus Paracoccidioides brasiliensis , is probably acquired inhalation of conidia mycelial form. The macrophage (Mφ) represents major cell defense against this pathogen; when activated with gamma interferon (IFN-γ), murine Mφs kill an oxygen-independent mechanism. Our goal was determine role nitric oxide in fungicidal effect on P. conidia. results revealed that IFN-γ-activated...

Using the intraperitoneal route of infection, we demonstrated previously that A/Sn mice are resistant and B10.A susceptible to Paracoccidioides brasiliensis infection. Since paracoccidioidomycosis is a deep systemic granulomatous disorder involves primarily lungs then disseminates other organs systems, herein investigated course infection resulting immune responses developed by after intratracheal with P. yeast cells. It was observed develop chronic benign pulmonary-restricted whereas...

We studied 52 patients with disseminated histoplasmosis, 30 the acquired immunodeficiency syndrome (AIDS) (cohort 1) and 22 not co-infected human virus 2). Demographic, clinical, laboratory, mycologic findings, as well antifungal therapy highly active antiretroviral (HAART), were analyzed. Skin lesions significantly higher in cohort 1 than 2 (P = 0.001). Anemia, leukopenia, an elevated erythrocyte sedimentation rate also more pronounced < Histoplasma capsulatum was isolated often 0.05)...

Melanins are implicated in the pathogenesis of several human diseases, including some microbial infections. In this study, we analyzed whether conidia and yeasts thermally dimorphic fungal pathogen Paracoccidioides brasiliensis produce melanin or melanin-like compounds vitro during infection. Growth P. mycelia on water agar alone produced pigmented conidia, growth minimal medium with L-3,4-dihydroxyphenylalanine (L-DOPA) cells. Digestion proteolytic enzymes, denaturant, hot concentrated acid...

The detection of coinfections is important to initiate appropriate antimicrobial therapy. Molecular diagnostic testing identifies pathogens at a greater rate than conventional microbiology. We assessed both bacterial identified via culture or the BioFire® FilmArray® Pneumonia Panel (FA-PNEU) in patients infected with SARS-CoV-2 ICU and concordance between these techniques.This was prospective study who were hospitalized for no more 48 h on mechanical ventilation longer 24 8 ICUs Medellín,...

Itraconazole effectively controls active paracoccidioidomycosis but appears not to hinder lung fibrosis. Clinical records and chest radiographs from 47 itraconazole-treated patients with prolonged posttherapy follow-up (mean period, 5.6 years) were analyzed; the interpreted following pneumoconiosis standards that consider lungs as 6 fields grade damage according number of involved. Infiltrative lesions observed at diagnosis in 93.6% patients. Fibrosis was 31.8% had cleared end observation...

ABSTRACT One of the most crucial events during infection with dimorphic fungus Paracoccidioides brasiliensis is adhesion to pulmonary epithelial cells, a pivotal step in establishment disease. In this study, we have evaluated relevance 32-kDa protein, putative member haloacid dehalogenase (HAD) superfamily hydrolases, virulence fungus. Protein sequence analyses supported inclusion PbHad32p as hydrolase and revealed conserved protein only among fungal filamentous pathogens that are closely...

Neutrophils play an important role as effector cells and contribute to the resistance of host against microbial pathogens. are able produce extracellular traps (NETs) in response medically fungi, including Aspergillus spp., Candida albicans Cryptococcus gattii. However, NET production Paracoccidioides brasiliensis has yet be studied. We have demonstrated that human neutrophils NETs both conidia yeasts P. brasiliensis. Although NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI) did...

Abstract The immunoprotective and immunomodulatory role of neutrophils during pulmonary infection resistant (A/J) susceptible (B10.A) mice to Paracoccidioides brasiliensis was investigated. First, comparative studies about early cellular influx the lungs demonstrated higher numbers in rather than mice. Neutrophil depletion resulted decreased survival times but not In both mouse strains, led increased fungal burdens at Week 1 infection; however, only remained with loads presented a dramatic...

A serologic study was undertaken in a group of 43 patients with active paracoccidioidomycosis who were treated the same form (ketoconazole), for identical periods time (6 months), and folio wed-up various posttherapy. The tests employed agar gel immunodiffusion (AGID) complement fixation (FC). Also studied 50 sera from proven histoplasmosis pulmonary aspergilloma, 30 culturaly tuberculosis as well 92 specimens healthy individuals, residents endemic area paracoccidioidomycosis. single lot...

A technique to collect and dislodge conidia produced by Paracoccidioides brasiliensis mycelial form Angela Restrepo, Restrepo 1Corporación para Investigaciones Biológicas, Hospital Pablo Tobón Uribe, Apartado Aéreo 73-78, Medellin, Colombia, South America Search for other works this author on: Oxford Academic PubMed Google Scholar Maria E. Salazar, Salazar Luz Cano, Cano M. Patiño Journal of Medical Veterinary Mycology, Volume 24, Issue 3, May 1986, Pages 247–250,...

ABSTRACT The conventional means of diagnosis histoplasmosis presents difficulties because the delay to time that is made, indicating need for implementation molecular assays. We evaluated 146 clinical samples from 135 patients suspected having using a previously reported nested PCR assay H istoplasma capsulatum -specific 100-kDa protein (the Hc100 PCR). In order determine specificity this test, we also used healthy individuals ( n = 20), respiratory disease with negative fungal cultures 29),...

Histoplasmosis causes a significant mortality, especially persons living with human immunodeficiency virus/acquired syndrome (HIV/AIDS) from developing countries where access to both appropriate diagnostic methods and antiretroviral therapy are limited. A total of 81 physicians assigned 17 Colombian departments (states) received training in the clinical, epidemiological, aspects histoplasmosis. Once this was during period October 2009–November 2012, these sent biological samples for...

Patients with paracoccidioidomycosis often present pulmonary fibrosis and exhibit important respiratory limitations. Based on an already established animal model, the contribution of viable non-viable P. brasiliensis propagules to development was investigated. BALB/c male mice, 4-6 weeks old were inoculated intranasally either 4x10(6 )viable conidia (Group I), or 6.5x10(6) fragmented yeast cells II). Control animals received PBS. Six mice per period sacrificed at 24, 48, 72h (initial) 1, 2,...

Using a pulmonary model of infection, we demonstrated previously that A/Sn and B10.A mice are, respectively, resistant susceptible to Paracoccidioides brasiliensis infection. Employing the same experimental model, examined herein role CD8(+) T cells in course paracoccidioidomycosis. Treatment with anti-CD8 monoclonal antibodies caused selective depletion splenic both mouse strains. The number CD4(+) immunoglobulin-positive was independent cells. In mice, loss by vivo treatment impaired...

Microorganisms adhere to extracellular matrix proteins by means of their own surface molecules. Paracoccidioides brasiliensis conidia have been shown be capable interacting with proteins. We aimed at determining the presence fungal that could interact protein and, if found, attempt purification and characterization. Various extracts were prepared from P. mycelial yeast cultures (total homogenates, beta-mercaptoethanol, sodium dodecyl sulfate [SDS] extracts) analyzed ligand affinity assays...

Autoimmune thrombocytopenia purpura in pregnancy is associated with fetal thrombocytopenia. Twenty-one patients autoimmune (22 fetuses) underwent 26 umbilical cord punctures to assess the platelet count. Blood could not be obtained from one fetus. Thrombocytopenia (less than 150,000/microL) was found five cases, but none it of sufficient degree preclude vaginal delivery. Persistent bradycardia necessitating emergency cesarean delivery occurred two cases. Umbilical puncture technically more...

The preliminary results of itraconazole therapy in 16 patients with active paracoccidioidomycosis were evaluated. course — administration for six months at a dose 100 mg per day-was completed 13 cases. This new triazole appeared as effective ketoconazole reducing the symptoms and arresting progression mycosis. scoring system employed to evaluate effect drug showed that condition no patient worsened or remained same during therapy. On contrary, 11 (84.6%) experienced major improvement other...

We determined the value of culture and serological tests used to diagnose histoplasmosis. The medical records 391 histoplasmosis patients were analyzed. Diagnosis mycosis was assessed by culture, complement fixation, immunodiffusion tests; 310 (79.5%) male, 184 (47.1%) infected with human immunodeficiency virus (HIV). Positivity for cultures 35.7% (74/207), reactivity 95.2% (160/168), a combination both methodologies 16.9% (35/207) non-HIV patients. 75.0% (138/184), 92.4% (85/92), 26.0%...

Pro-inflammatory cytokines play an important role in both recruitment and activation of leukocytes migrating into tissues response to invading pathogens. In this study the production pro-inflammatory cytokines, determined by ELISA assays, lungs BALB/c mice infected with Paracoccidioides brasiliensis conidia were evaluated during early stages infection. The results showed that had a significant increase lung first 4 days peak at day 2 post-challenge; infiltrates composed mainly...