A5101970409- Asthma and respiratory diseases
- Immune Response and Inflammation
- Allergic Rhinitis and Sensitization
- Trypanosoma species research and implications
- Immune Cell Function and Interaction
- Research on Leishmaniasis Studies
- IL-33, ST2, and ILC Pathways
- Immune cells in cancer
- Fungal Infections and Studies
- Food Allergy and Anaphylaxis Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immunotherapy and Immune Responses
- Pediatric health and respiratory diseases
- T-cell and B-cell Immunology
- Antifungal resistance and susceptibility
- Nail Diseases and Treatments
- Nitric Oxide and Endothelin Effects
- Cytokine Signaling Pathways and Interactions
- Receptor Mechanisms and Signaling
- SARS-CoV-2 and COVID-19 Research
- Olfactory and Sensory Function Studies
- Neonatal Respiratory Health Research
- Cell Adhesion Molecules Research
- Antimicrobial Peptides and Activities
- Hydrogen's biological and therapeutic effects
Universidade de São Paulo
2015-2024
Fundação Oswaldo Cruz
2023
Universidade Federal do Rio de Janeiro
2023
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2023
Universidade Federal do Amazonas
2023
Institute of Biomedical Science
1997-2018
Istituto Zooprofilattico Sperimentale Della Sicilia
2012
Instituto Butantan
1993-2010
Universidade Brasil
1988-2005
Institut Pasteur
2001
Foxp3 + CD25 CD4 regulatory T cells are vital for peripheral tolerance and control of tissue inflammation. In this study, we characterized the phenotype monitored migration activity present in airways allergic or tolerant mice after allergen challenge. To induce lung inflammation, were sensitized twice with ovalbumin/aluminum hydroxide gel challenged intranasal ovalbumin. Tolerance was induced by oral administration ovalbumin 5 consecutive days prior to OVA sensitization We detected (Foxp3...
Airway hyperreactivity (AHR), eosinophilic inflammation with a Th2-type cytokine profile, and specific Th2-mediated IgE production characterize allergic asthma. In this paper, we show that OVA-immunized Jalpha18(-/-) mice, which are exclusively deficient in the invariant Valpha14(+) (iValpha14), CD1d-restricted NKT cells, exhibit impaired AHR airway eosinophilia, decreased IL-4 IL-5 bronchoalveolar lavage fluid, reduced OVA-specific compared wild-type (WT) littermates. Adoptive transfer of...
Abstract Serum amyloid A (SAA), a classical acute-phase protein, is produced predominantly by hepatocytes in response to injury, infection, and inflammation. It has been shown that SAA primes leukocytes induces the expression release of proinflammatory cytokines. Here, we report NO production murine peritoneal macrophages. Using specific inhibitors, showed was dependent on inducible synthase thorough activation ERK1/2 p38 MAPKs. Moreover, activity decreased after proteolysis but not with...
Malaria remains one of the greatest burdens to global health, causing nearly 500,000 deaths in 2014. When manifesting lungs, severe malaria causes acute lung injury/acute respiratory distress syndrome (ALI/ARDS). We have previously shown that a proportion DBA/2 mice infected with Plasmodium berghei ANKA (PbA) develop ALI/ARDS and these recapitulate various aspects human syndrome, such as pulmonary edema, hemorrhaging, pleural effusion hypoxemia. Herein, we investigated role neutrophils...
Asthma results from an intrapulmonary allergen-driven Th2 response and is characterized by intermittent airway obstruction, hyperreactivity, inflammation. An inverse association between allergic asthma microbial infections has been observed. Microbial could prevent responses inducing the secretion of type 1 cytokines, IL-12 IFN-gamma. In this study, we examined whether administration bacterial LPS, a prototypic product that activates innate immune cells via Toll-like receptor 4 (TLR4)...
Objective: We have documented an impaired neutrophil migration toward the infectious focus in severe sepsis. This phenomenon appears to be mediated by nitric oxide, release of which is stimulated circulating inflammatory cytokines released immune cells after stimulation bacteria and/or their products. Toll-like receptor 4 (TLR4) major recognition for lipopolysaccharide, a component Gram-negative bacterial cell walls. In present study, we investigated whether TLR4 involved failure mice...
Abstract Using a murine model of susceptibility and resistance to paracoccidioidomycosis, we have previously demonstrated that immunosuppression occurs in susceptible (B10.A), but not resistant (A/Sn), mouse strains. Accumulating evidence shows NO is involved the induction T cell during infection as well killing Paracoccidioides brasiliensis. In present work, focused on other macrophage products could be associated with or paracoccidioidomycosis. A striking difference was related TNF...
Using the intraperitoneal route of infection, we demonstrated previously that A/Sn mice are resistant and B10.A susceptible to Paracoccidioides brasiliensis infection. Since paracoccidioidomycosis is a deep systemic granulomatous disorder involves primarily lungs then disseminates other organs systems, herein investigated course infection resulting immune responses developed by after intratracheal with P. yeast cells. It was observed develop chronic benign pulmonary-restricted whereas...
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We investigated the role of Fas ligand in murine silicosis. Wild-type mice instilled with silica developed severe pulmonary inflammation, local production tumor necrosis factor (TNF)-α, and interstitial neutrophil macrophage infiltration lungs. Strikingly, ligand–deficient generalized lymphoproliferative disease mutant (gld) did not develop The gld had markedly reduced extravasation into bronchoalveolar space, show increased TNF-α production, nor inflammation. Bone marrow chimeras adoptive...
Abstract Leukotrienes (LTs) are known to be produced by macrophages when challenged with Leishmania, but it is not whether these lipid mediators play a role in host defense against this important protozoan parasite. In study, we investigated the involvement of LTs vitro and vivo response Leishmania amazonensis infection susceptible (BALB/c) resistant (C3H/HePAS) mice. Pharmacologic or genetic deficiency resulted impaired leishmanicidal activity peritoneal vitro. contrast, addition LTB4...
Epidemiological and experimental data suggest that bacterial lipopolysaccharides (LPS) can either protect from or exacerbate allergic asthma. Lipopolysaccharides trigger immune responses through toll-like receptor 4 (TLR4) in turn activates two major signalling pathways via MyD88 TRIF adaptor proteins. The LPS is a pro-Type 1 T helper cells (Th1) adjuvant while aluminium hydroxide (alum) strong Type 2 (Th2) adjuvant, but the effect of mixing both adjuvants on development lung allergy has not...
We have previously shown that regulatory T (Treg) cells accumulate in the airways of allergic mice upregulate CC-chemokine receptor 4 (CCR4) expression. These Treg suppressed vitro Th2 cell proliferation but not type 2 cytokine production. In current study, using a well-established murine model lung disease or oral tolerance, we evaluated vivo activity airway inflammation with special focus on CCR4 function. found allergic, tolerant, treated anti-CD25 Ab showed increased eosinophilia and...
Inhaled bacterial lipopolysaccharides (LPSs) induce an acute tumour necrosis factor-alpha (TNF- α -) dependent inflammatory response in the murine airways mediated by Toll-like receptor 4 (TLR4) via myeloid differentiation MyD88 adaptor protein pathway. However, contractile of bronchial smooth muscle and role endogenous TNF this process have been elusive. We determined vivo respiratory pattern C57BL/6 mice after intranasal LPS administration with or without presence increasing doses...
Aluminum-containing adjuvants usually referred as Alum are considered T helper type-2 (Th2) adjuvants, while agonists of toll-like receptors (TLRs) viewed that favor Th1/Th17 immunity. has been used in numerous vaccine formulations; however, its undesired pro-Th2 adjuvant activity constitutes a caveat for Alum-based vaccines. Combining with TLR-dependent, pro-Th1/Th17 might dampen the and improve effectiveness formulations. Here, using ovalbumin (OVA) model allergic lung inflammation, we...
Obesity and type 2 diabetes (T2D) have been found to be associated with abnormalities in several organs, including the intestine. These conditions can lead changes gut homeostasis, compromising tolerance luminal antigens increasing susceptibility food allergies. The underlying mechanisms for this phenomenon are not yet fully understood. In study, we investigated intestinal mucosa of diet-induced obese mice that they exhibited increased permeability reduced Treg cells frequency. Upon oral...
Abstract Effective antibody responses are essential to generate protective humoral immunity. Different inflammatory signals polarize T cells towards appropriate effector phenotypes during an infection or immunization. Th1 and Th2 have been associated with the polarization of responses. However, follicular helper (Tfh) a unique ability access B cell follicle support germinal center (GC) by providing help. We investigated specialization Tfh induced under type-1 type-2 conditions. first studied...