A5101807675- Trace Elements in Health
- Drug Transport and Resistance Mechanisms
- Renin-Angiotensin System Studies
- Heavy Metal Exposure and Toxicity
- Iron Metabolism and Disorders
- Immune cells in cancer
- Protein Hydrolysis and Bioactive Peptides
- Nitric Oxide and Endothelin Effects
- Ion-surface interactions and analysis
- Hemoglobinopathies and Related Disorders
- Aortic aneurysm repair treatments
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Fungal Diseases
- Pregnancy and preeclampsia studies
- Cholesterol and Lipid Metabolism
- Apelin-related biomedical research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Heat shock proteins research
- Orthopaedic implants and arthroplasty
- Biochemical and Structural Characterization
- Protease and Inhibitor Mechanisms
- Connective tissue disorders research
- Pharmacological Effects of Medicinal Plants
- Williams Syndrome Research
- Nanocomposite Films for Food Packaging
The University of Texas Health Science Center at San Antonio
2010-2015
University of Cincinnati Medical Center
2008-2013
University of Cincinnati
2007-2012
Analytical Engineering (United States)
2012
Forschungszentrum Jülich
2010
In-Q-Tel
2010
Sabin Vaccine Institute
2009
Emory University
2005-2008
University of Illinois Chicago
2008
Shenzhen Polytechnic
2007
Abstract Until recently, Histoplasma capsulatum was believed to harbour three varieties, var. (chiefly a New World human pathogen), duboisii (an African pathogen) and farciminosum Old horse which varied in clinical manifestations geographical distribution. We analysed the phylogenetic relationships of 137 individuals representing varieties from six continents using DNA sequence variation four independent protein‐coding genes. At least eight clades were idengified: (i) North American class 1...
We previously found that angiotensin II–induced hypertension increases vascular extracellular superoxide dismutase (ecSOD), and proposed this is a compensatory mechanism blunts the hypertensive response preserves endothelium-dependent vasodilatation. To test hypothesis, we studied ecSOD-deficient mice. ecSOD −/− C57Blk/6 mice had similar blood pressure at baseline; however, caused by II was greater in compared with wild-type (168 versus 147 mm Hg, respectively; P <0.01). In keeping this,...
Extracellular superoxide dismutase (SOD3), a secretory copper enzyme, plays an important role in atherosclerosis and hypertension by modulating the levels of extracellular anion (O2*-) vasculature. Little is known about mechanisms which SOD3 obtains its catalytic cofactor. Menkes ATPase (MNK) has been shown to transport cytosolic pathway nonvascular cells. We performed present study determine whether MNK required for activation Here we show that was highly expressed various vascular tissues...
Bioimaging mass spectrometric techniques allow direct mapping of metal and biomolecule distributions with high spatial resolution in biological tissue. In this study laser ablation inductively coupled plasma spectrometry (LA-ICPMS) was used for imaging transition metals (Fe, Cu, Zn, Mn, Ti), alkali alkaline-earth (Na, K, Mg, Ca, respectively), selected nonmetals (such as C, P, S) native cryosections mouse heart. The nonmetal images clearly illustrated the shape anatomy samples. Zinc copper...
Background Recruitment of macrophage precursors to the adventitia plays a key role in pathogenesis abdominal aortic aneurysms ( AAA s), but molecular mechanisms remain undefined. The innate immune signaling molecule CD 14 was reported be upregulated adventitial macrophages murine model and monocytes cocultured with fibroblasts (AoAf) vitro , concurrent increased interleukin‐6 (IL‐6) expression. We hypothesized that crucial precursor recruitment early during formation. Methods Results −/−...
The extracellular superoxide dismutase (SOD3), a secretory copper-containing enzyme, regulates angiotensin II (Ang II)-induced hypertension by modulating levels of anion. present study was designed to determine the role copper transporter Menkes ATPase (MNK) in Ang II-induced SOD3 activity and vivo. Here we show that chronic infusion enhanced systolic blood pressure vascular anion production MNK mutant (MNK(mut)) mice as compared with those wild-type mice, which are associated impaired...
Understanding of spatiotemporal profiling inflammatory mediators and their associations with MΦ accumulation is crucial to elucidate the complex immune properties. Here, we used murine thioglycollate elicited peritonitis determine concentrations 23 in peritoneal exudates plasma before (day 0) after (days 1 3) administration cavities; these included TNF- α , FGF-9, IFN- γ IP-10, RANTES, IL-1 IL-6, IL-7, IL-10, IL-11, IL-12p70, IL-17A, lymphotactin, OSM, KC/GRO, SCF, MIP-1 β MIP-2, TIMP-1,...
Copper and zinc are essential trace biometals that regulate cardiovascular homeostasis, dysregulation of these metals has been linked to vascular diseases, including hypertension. In this article, we review recent human population studies concerning topic, focusing on: 1) the relationship between blood pressure levels copper; 2) correlations metals, renin-angiotensin system, obesity, hypertension; 3) environmental metal pollution development 4) methods commonly employed assay copper in...
An antihypertensive peptide, Lys-Val-Leu-Pro-Val-Pro (KVLPVP), can reduce blood pressure in hypertensive rats after being orally administered. In this study, the transepithelial transport of intact KVLPVP was examined by Caco-2 monolayers. The results were as follows: (i) flux not saturable for apical (AP) to basolateral (BL) or BL-AP when concentration 1–8 mm. (ii) Sodium deoxycholate loosened tight junction cells and significantly improved process. (iii) Phenylarsine oxide, a transcytotic...
An optimal antihypertensive peptide (AHP), KVLPVP, was linked to form a six-copy of tandem dotetracontapeptide with the specific cleavage site (Arg-X) clostripain. The gene synthesized and expressed in Escherichia coli BL21. After 5 h induction 1.2 mM isopropyl-β-d-thiogalactopyranoside recombinant AHP fused glutathione-S-transferase tag reached maximal production, 24.6% total intracellular protein. Following digestion clostripain carboxypeptidase B, product separated ultrafiltration...
ATP7A primarily functions to egress copper from cells, thereby supplying this cofactor secreted copper-accepting enzymes. This ATPase has attracted significant attention since the discovery of its mutation leading human Menkes disease and demonstration distribution in various tissues. Recently, we reported that is expressed vasculature. In present study, investigated cellular expression atherosclerotic lesions LDL receptor (-/-) mice. Subsequently, examined role regulating oxidation a...
Copper, an essential trace metal in humans, plays important role elastic formation. However, little is known about the spatial association between copper, elastin, and elastin producing cells. The aorta largest artery; aortic media primarily composed of lamellae vascular smooth muscle cells, which makes it a good model to address this issue. Synchrotron radiation X-ray fluorescence microscopy (SRXRF) new generation technique investigate topography metals biological samples. Recently, we...
Recently, using synchrotron radiation X-ray fluorescence microscopy (SRXRF), the copper accumulation in rat aortic elastin and topography human THP-1 cell monolayer have been described. However, it is necessary to locate more accurately cellular vascular cells tissues. In current study, SRXRF coupling with transmission electron (TEM) was used image sections of mouse aorta. The results showed that 1 µm thickness are required for producing a correlative TEM between ultrastructure. As compared...