A5055451292- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Infectious Diseases and Mycology
- Yeasts and Rust Fungi Studies
- Genetic Associations and Epidemiology
- Mycorrhizal Fungi and Plant Interactions
- Nutrition, Genetics, and Disease
- Antimicrobial Peptides and Activities
- Probiotics and Fermented Foods
- Pneumocystis jirovecii pneumonia detection and treatment
- Biochemical and Structural Characterization
- Venomous Animal Envenomation and Studies
- Plant Disease Resistance and Genetics
- Medical and Biological Ozone Research
- Glycosylation and Glycoproteins Research
- Fungal Biology and Applications
- Bacterial Identification and Susceptibility Testing
- Mycotoxins in Agriculture and Food
- Genomic variations and chromosomal abnormalities
- Protist diversity and phylogeny
- Plant Pathogens and Resistance
- Nail Diseases and Treatments
- Bioinformatics and Genomic Networks
Corporación para Investigaciones Biológicas
2013-2025
Oak Ridge Institute for Science and Education
2024
Centers for Disease Control and Prevention
2021-2024
Oak Ridge Associated Universities
2024
CDC Foundation
2023
Universidad Nacional de Colombia
2020
Universidad de Antioquia
2013-2020
IRCCS Istituto Auxologico Italiano
2016
Istituti di Ricovero e Cura a Carattere Scientifico
2016
Universidad del Rosario
2016
Abstract Background Candida auris is a multidrug-resistant yeast associated with hospital outbreaks worldwide. During 2015–2016, multiple were reported in Colombia. We aimed to understand the extent of contamination healthcare settings and characterize molecular epidemiology C. Methods sampled patients, patient contacts, workers, environment 4 hospitals recent outbreaks. Using standardized protocols, people swabbed at different body sites. Patient procedure rooms sectioned into zones...
Characterization of genetic differences between lineages the dimorphic human-pathogenic fungus Paracoccidioides can identify changes linked to important phenotypes and guide development new diagnostics treatments. In this article, we compared genomes 31 diverse isolates representing major spp. completed first annotated genome sequences for PS3 PS4 lineages. We analyzed population structure characterized diversity among , including a deep split S1 into two (S1a S1b), differentiated S1b,...
Zoonotic sporotrichosis is a neglected fungal disease, whereby outbreaks are primarily driven by Sporothrix brasiliensis and linked to cat-to-human transmission. To understand the emergence spread of in Brazil, epicentre current epidemic South America, we aimed conduct whole-genome sequencing (WGS) describe genomic epidemiology.
In our study, mutations associated to azole resistance and echinocandin were detected in Candida glabrata isolates using a whole-genome sequence. C. is the second most common cause of candidemia United States, which rapidly acquires antifungals, vitro vivo .
Abstract Background Reports of fluconazole-resistant Candida parapsilosis bloodstream infections are increasing. We describe a cluster C identified in 2021 on routine surveillance by the Georgia Emerging Infections Program conjunction with Centers for Disease Control and Prevention. Methods Whole-genome sequencing was used to analyze isolates. Epidemiological data were obtained from medical records. A social network analysis conducted using Hospital Discharge Data. Results Twenty isolates...
Three closely related thermally dimorphic pathogens are causal agents of major fungal diseases affecting humans in the Americas: blastomycosis, histoplasmosis and paracoccidioidomycosis. Here we report genome sequence analysis four strains etiological agent Blastomyces, two species genus Emmonsia, typically small mammals. Compared to species, Blastomyces genomes highly expanded, with long, often sharply demarcated tracts low GC-content sequence. These GC-poor isochore-like regions enriched...
Since 2016, in Colombia, ongoing transmission of
Paracoccidiodomycosis (PCM) is a clinically important fungal disease that can acquire serious systemic forms and caused by the thermodimorphic Paracoccidioides spp. PCM tropical endemic in Latin America, where up to ten million people are infected; 80% of reported cases occur Brazil, followed Colombia Venezuela. To enable genomic studies better characterize pathogenesis this dimorphic fungus, two reference strains P. brasiliensis (Pb03, Pb18) one strain lutzii (Pb01) were sequenced [1]....
The genus Aspergillus comprises a diverse group of fungi that can cause range health issues, including systemic infections and allergic reactions. In this regard, A. fumigatus has been recognized as the most prevalent allergen-producing species. This taxonomic classification subject to frequent updates, which generated considerable difficulties for its when traditional identification methodologies are employed. To demonstrate feasibility approach, we sequenced whole genomes 81 isolates...
We used metagenomic next-generation sequencing (mNGS) to investigate an outbreak of Fusarium solani meningitis in US patients who had surgical procedures under spinal anesthesia Matamoros, Mexico, during 2023. Using a novel method called metaMELT (metagenomic multiple extended locus typing), we performed phylogenetic analysis concatenated mNGS reads from 4 (P1-P4) parallel with 28 fungal reference genomes. Fungal strains the were most closely related each other and 2 cultured isolates P1...
is an emerging zoonotic fungal pathogen that can be difficult to treat. Antifungal susceptibility testing was performed on the mold phase of a convenience sample 61
Candida auris is a multidrug-resistant pathogen that represents serious public health threat due to its rapid global emergence, increasing incidence of healthcare-associated outbreaks, and high rates antifungal resistance. Whole-genome sequencing genomic surveillance have the potential bolster C. networks moving forward. Laboratories conducting need be able compare analyses from various national international partners ensure results are mutually trusted understood. Therefore, we established...
Sporothrix schenckii is a thermodimorphic fungal pathogen with high genetic diversity. In this work, we present the assembly and similarity analysis of whole-genome sequences two clinical isolates from Colombia S. schenckiisensu stricto.
Abstract The LUFS domain (LUG/LUH, Flo8, single‐strand DNA‐binding protein [SSBP]) is a well‐conserved and apparently ancient region found in diverse proteins taxa. This domain, which has as its most obvious structural feature series of three helices, been identified transcriptional regulator animals, plants, fungi. Recently, these pages (Wang et al., Protein Sci ., 2019, 28:788–793), the first crystal structure was reported, for human SSBP2, repressor. We briefly address how new insights...
Candida auris is an emerging fungal pathogen capable of causing invasive infections in humans. Since its first appearance around 1996, it has been isolated countries spanning five continents. C. a yeast that the potential to cause outbreaks hospitals, can survive adverse conditions, including dry surfaces and high temperatures, frequently misidentified by traditional methods. Furthermore, strains have identified are resistant two even all three main classes antifungals currently use. Several...
The mitochondrial genome of the Paracoccidioides brasiliensis reference isolate Pb18 was first sequenced and described by Cardoso et al. in 2007, as a circular with size 71.3 kb containing 14 protein coding genes, 25 tRNAs large small subunits ribosomal RNA. Later 2011, Desjardins obtained partial assemblies genomes P. lutzii (Pb01), Americana (Pb03) sensu stricto (Pb18), although only 43.1 for Pb18. Sequencing errors or other limitations resulting from earlier technologies, advantages NGS...
Spider venoms constitute a trove of novel peptides with biotechnological interest. Paucity next-generation-sequencing (NGS) data generation has led to description less than 1% these peptides. Increasing evidence supports the underestimation assembled genes single transcriptome assembler can predict. Here, venom gland spider Pamphobeteus verdolaga was re-assembled, using three free access algorithms, Trinity, SOAPdenovo-Trans, and SPAdes, obtain more complete annotation. Assembler’s...
The band 9p21.3 contains an established genomic risk zone for cardiovascular disease (CVD). Since the initial 2007 Wellcome Trust Case Control Consortium study (WTCCC), increased CVD associated with has been confirmed by multiple studies in different continents. However, many years later there was still no report of a corresponding association hypertension, major CV factor, nor blood pressure (BP). In this contribution, we review bipartite haplotype structure locus: one block is devoid...
In recent years, readily affordable short read sequences provided by next‐generation sequencing (NGS) have become longer and more accurate. This has led to a jump in interest the utility of NGS‐only approaches for exploring eukaryotic genomes. The concept static, ‘finished’ genome assembly, which still appears be faraway goal many eukaryotes, is yielding new paradigms. We here motivate an object‐view where raw reads are main, fixed object, assemblies with their annotations take role...