A5058131882- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Antimicrobial Peptides and Activities
- Plant Pathogens and Fungal Diseases
- Bacterial Identification and Susceptibility Testing
- Oral microbiology and periodontitis research
- Peptidase Inhibition and Analysis
- Probiotics and Fermented Foods
- Yeasts and Rust Fungi Studies
- Fungal Biology and Applications
- Pneumocystis jirovecii pneumonia detection and treatment
- Essential Oils and Antimicrobial Activity
- Antimicrobial agents and applications
- Biochemical and Structural Characterization
- Protein Hydrolysis and Bioactive Peptides
- Streptococcal Infections and Treatments
- Microbial Natural Products and Biosynthesis
- Antimicrobial Resistance in Staphylococcus
- Chemical Synthesis and Analysis
- Oral Health Pathology and Treatment
- Nail Diseases and Treatments
- Bacterial Infections and Vaccines
- Infective Endocarditis Diagnosis and Management
- Advanced Drug Delivery Systems
- Fungal and yeast genetics research
Pontificia Universidad Javeriana
2016-2025
Universidad Europea
2025
Universidad Complutense de Madrid
2014-2024
Universidad Nacional de Colombia
2016-2024
Universidad Tecnológica de Bolívar
2022-2023
University of Boyaca
2022-2023
Instituto Nacional de Salud
2023
Universidad del Sinú
2022
Universidad El Bosque
2022
Hospital Universitario San Ignacio
2018-2020
Abstract Background Candida auris is a multidrug-resistant yeast associated with hospital outbreaks worldwide. During 2015–2016, multiple were reported in Colombia. We aimed to understand the extent of contamination healthcare settings and characterize molecular epidemiology C. Methods sampled patients, patient contacts, workers, environment 4 hospitals recent outbreaks. Using standardized protocols, people swabbed at different body sites. Patient procedure rooms sectioned into zones...
The commensal fungus Candida albicans secretes a considerable number of proteins and, as in different fungal pathogens, extracellular vesicles (EVs) have also been observed. Our report contains the first proteomic analysis EVs C. and comparative study soluble secreted proteins. With this purpose, cell-free culture supernatants from were separated into EV-free supernatant analyzed by LC–MS/MS. A total 96 identified including 75 61 supernatant, respectively. Out these, 40 found secretome for...
Candida auris has become a major health threat due to its transmissibility, multidrug resistance and severe outcomes. In case-control design, 74 hospitalised patients with candidemia were enrolled. total, 22 cases (29.7%) 52 controls (C. albicans, 21.6%; C. parapsilosis, tropicalis, glabrata, 1.4%) included analysed in this study. Risk factors, clinical microbiological characteristics outcomes of non-auris species (NACS) compared. Previous fluconazole exposure was significantly higher (OR...
Over a four-year period, 123 Candida bloodstream isolates were collected at quaternary care hospital. The identified by MALDI-TOF MS and their fluconazole (FLC) susceptibility patterns assessed according to CLSI guidelines. Subsequently, sequencing of ERG11, TAC1 or MRR1, efflux pump activity performed for resistant isolates.
BackgroundCandida auris is a recently reported Candida species that phenotypically similar to haemulonii and related hospital outbreaks. This organism can be misidentified as haemulonii, famata, catenulata, or Rhodotorula glutinis by phenotypic approaches. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) DNA sequence analysis using internal transcribed spacer rDNA bar-coding provide an accurate identification.Case reportsThree cases of C. infection...
Candida auris, first described in 2009, is an opportunistic pathogenic yeast that causes nosocomial outbreaks around the world, with high mortality rates associated therapeutic failure. In this study, we evaluated pathogenicity of 107 isolates from two cities Colombia, fungemia or colonization processes; to achieve this, used Galleria mellonella invertebrate model compare pathogenicity. Our results showed less than half total C. auris presented a compared reference strain SC5314, and most...
Aim: To identify peptides derived from bovine lactoferricin (LfcinB) as potential therapeutics for colon cancer treatment. We systematically modified dimeric to enhance their selectivity against cells and reduce toxicity. examined the effects of specific changes, such substituting L-arginine (Arg) with L-ornithine (Orn) and/or D-Arg, on cytotoxic activity in cells, well prostate cervical cell lines. Additionally, we assessed type death induced vivo toxicity peptides. Methods: The were...
This study reports the synthesis, characterization, and antifungal evaluation of a series pyridoxal salicylaldehyde derivatives, using synthetic methodologies such as radical cyclizations click chemistry. Compounds 6a 6b, featuring fused dihydrobenzoxepine-pyridine scaffold, demonstrated effective fungicidal activity with MIC values 19 µg/mL against Cryptococcus neoformans 2807. Similarly, compound 6b exhibited notable 75 Candida auris PUJ-HUSI 537. Both compounds outperformed fluconazole...
Ecm33 is a glycosylphosphatidylinositol-anchored protein in the human pathogen Candida albicans. This known to be involved fungal cell wall integrity (CWI) and also critical for normal virulence mouse model of hematogenously disseminated candidiasis, but its function remains unknown. In this work, several phenotypic analyses C. albicans ecm33/ecm33 mutant (RML2U) were performed. We observed that RML2U displays inability protoplast regenerate wall, activation CWI pathway, hypersensitivity...
Candida auris and haemulonii complex (C. haemulonii, C. var. vulnera duobushaemulonii) are phylogenetically related species that share some physiological features habits. In the present study, we compared virulence of these yeast using two different experimental models: (i) Galleria mellonella larvae to evaluate survival rate, fungal burden, histopathology phagocytosis index (ii) BALB/c mice survival. addition, capacity form biofilm over an inert surface was analyzed. Our results showed in...
Objective The aim of this study was to analyze the cultivable oral microbiota patients with obstructive sleep apnea (OSA) and its association periodontal condition. Methods epidemiology profile their clinical characteristics were determined. collected from saliva, subgingival plaque, gingival sulcus 93 classified into four groups according diagnosis: Group 1 ( n = 25), healthy patients; 2 17), periodontitis without OSA; 3 19), OSA periodontitis; 4 32), OSA. Microbiological samples cultured,...
This study aims to compare the salivary and gingival crevicular fluid (GCF) concentrations of five cytokines: IL-1β, IL-6, IL-17A, IL-33, Tumor Necrosis Factor-alpha (TNF-α) in patients with OSA their association periodontitis.Samples saliva GCF were obtained from 84 classified into four groups according periodontal diagnosis: G1(H) healthy patients, G2(P) periodontitis non-OSA G3(OSA) non-periodontitis G4(P-OSA) patients. The cytokines samples quantified using multiplexed bead immunoassays....
Periodontitis has been linked to obstructive sleep apnea (OSA). The stage III periodontitis is more prevalent in OSA severe and the oral microbiome changes due an increase periodontal pathogenic bacteria C. albicans. Nanofiber drug delivery systems may be used for local antimicrobial therapy against microorganisms related OSA. aim of present study was manufacture PCL/PLGA nanofibers incorporating antifungal, Amphotericin B (AMB); peptide, LfcinB (21–25)Pal (P61); zinc oxide (ZnO), inhibition...
Invasive fungal disease causes high morbidity and mortality among immunocompromised patients. Resistance to conventional antifungal drugs the toxicity associated with doses highlight need for effective therapies. In this study, potential of ethanolic extract
It was previously reported that the peptide derived from LfcinB, R-1-R exhibited anti-Candida activity and this enhanced in combination with an extract Bidens pilosa plant. However, mechanism of action has not been studied. In current study, a proteomic study carried out, followed by bioinformatic analysis biological assays both SC5314 strain fluconazole-resistant isolate Candida albicans, after incubation R-1-R. The data showed treatment two strains, most differentially expressed proteins...
Candida albicans is a human opportunistic fungus and it responsible for wide variety of infections, either superficial or systemic. C. polymorphic its ability to switch between yeast hyphae essential virulence. Once obtains access the body, host serum constitutes complex environment interaction with cell surface in bloodstream. To draw comprehensive picture this relevant step host-pathogen during invasive candidiasis, we have optimized gel-free shaving proteomic strategy identify both,...
Amphotericin B (AmB) is a broad spectrum of antifungal drug used to treat diseases. However, due the high toxicity AmB, treated patients may suffer risk side effects, such as renal failure. Nanoencapsulation strategies have been reported elicit low toxicity, albeit most them possess encapsulation efficiency. The aim this research develop micellar delivery systems for AmB with reduced while maintaining its affectivity by employing retinol (RET)-conjugated amphiphilic block copolymers (ABCs)...
Malassezia furfur and pachydermatis are lipophilic lipid dependent yeasts, associated with the skin microbiota in humans domestic animals, respectively. Although they commensals under specific conditions become pathogens, causing conditions, such as pityriasis versicolor, dandruff/seborrheic dermatitis, folliculitis humans, dermatitis otitis dogs. Additionally, these species fungemia immunocompromised patients low-weight neonates intensive care units intravenous catheters or parenteral...
Breast cancer is one of the main causes premature death in women; current treatments have low selectivity, generating strong physical and psychological sequelae. The palindromic peptide R-1-R (RWQWRWQWR) has cytotoxic activity against different cell lines derived from selectivity noncancerous cells. To determine if changes charge/length this increase its activity, six peptides were obtained by SPPS, three them with addition Arg at N, C-terminal or both deletion both. selective activities...
The opportunistic fungal pathogens belonging to the Candida haemulonii complex and phylogenetically related species auris are well-known for causing infections that difficult treat due their multidrug-resistance profiles. is even more worrisome its ability cause outbreaks in healthcare settings. These emerging yeasts produce a wide range of virulence factors facilitate development infectious process. In recent years, aggregative phenotype has been receiving attention, as it mainly associated...