A5053624702- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Lung Cancer Treatments and Mutations
- HER2/EGFR in Cancer Research
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Axon Guidance and Neuronal Signaling
- Renal cell carcinoma treatment
- RNA Interference and Gene Delivery
- Neuroendocrine Tumor Research Advances
- Lung Cancer Research Studies
- Virus-based gene therapy research
- Renal and related cancers
- Genomics, phytochemicals, and oxidative stress
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
- Glutathione Transferases and Polymorphisms
- Genomics and Chromatin Dynamics
- Microbial Inactivation Methods
- Cancer Cells and Metastasis
- Histone Deacetylase Inhibitors Research
Università Campus Bio-Medico
2016-2025
Istituto di Farmacologia Traslazionale
2020-2025
National Research Council
2020-2025
Istituti di Ricovero e Cura a Carattere Scientifico
2014-2024
Casa Sollievo della Sofferenza
2015-2024
PCR Oncology
2013-2023
Sapienza University of Rome
1987-2021
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2021
University of Milan
2021
Frederick National Laboratory for Cancer Research
2018
Colorectal cancer (CRC) is a major health problem in industrialized countries. Although inflammation-linked carcinogenesis well accepted concept and often observed within the gastrointestinal tract, underlying mechanisms remain to be elucidated. Inflammation can indeed provide initiating promoting stimuli mediators, generating tumour-prone microenvironment. Many murine models of sporadic inflammation-related colon have been developed last decade, including chemically induced CRC models,...
We discovered a novel somatic gene fusion, CD74-NRG1, by transcriptome sequencing of 25 lung adenocarcinomas never smokers. By screening 102 negative for known oncogenic alterations, we found four additional fusion-positive tumors, all which were the invasive mucinous subtype. Mechanistically, CD74-NRG1 leads to extracellular expression EGF-like domain NRG1 III-β3, thereby providing ligand ERBB2-ERBB3 receptor complexes. Accordingly, ERBB2 and ERBB3 was high in index case, phospho-ERBB3...
The KEAP1/Nrf2 pathway is a master regulator of several redox-sensitive genes implicated in resistance tumor cells against chemotherapeutic drugs. Recent data suggest that epigenetic mechanisms may play pivotal role the regulation KEAP1 expression. We performed comprehensive genetic and analysis gene 47 non-small cell lung cancer tissues normal specimens. Promoter methylation was using quantitative specific PCR assay real time. Methylation at promoter region detected 22 out NSCLCs (47%) none...
Abstract Purpose: In an effort to additionally determine the global patterns of CpG island hypermethylation in sporadic breast cancer, we searched for aberrant promoter methylation at 10 gene loci 54 primary cancer and benign lesions. Experimental Design: Genomic DNA sodium bisulfate converted from malignant tissues was used as template methyl-specific PCR BRCA1, p16, ESR1, GSTP1, TRβ1, RARβ2, HIC1, APC, CCND2, CDH1 genes. Results: The majority (85%) showed least 1 tested with half them...
A new human retrovirus was isolated from a continuous cell line derived patient with CD4 + Tac - cutaneous T lymphoma/leukemia. This virus is related to but distinct leukemia/lymphoma types I and II (HTLV-I HTLV-II) immunodeficiency (HIV-1). With the use of fragment provirus cloned one leukemia, closely sequences were found in DNA tumor cells seven other patients same disease; these only distantly HTLV-I. The phenotype clinical course disease clearly distinguishable leukemia associated All...
In light with the view that KEAP1 loss of function may impact tumour behavior and modify response to chemotherapeutical agents, we sought determine whether gene is epigenetically regulated in malignant gliomas. We developed a Quantitative Methylation Specific PCR (QMSP) assay analyze 86 gliomas 20 normal brain tissues. The discriminatory power was assessed by Receiving Operating Characteristics (ROC) curve analysis. AUC value 0.823 (95%CI: 0.764-0.883) an optimal cut off 0.133 yielding 74%...
Keap1 (Kelch-like ECH-associated protein 1) is an adaptor that mediates the ubiquitination/degradation of genes regulating cell survival and apoptosis under oxidative stress conditions. We determined methylation status KEAP1 promoter in 102 primary breast cancers, 14 pre-invasive lesions, 38 paired normal tissues 6 from reductive mammoplasty by quantitative specific PCR (QMSP). Aberrant was detected 52 out cancer cases (51%) 10 lesions (71%). No mutations gene were identified 20 analyzed...
The Keap1/Nrf2 pathway is a master regulator of the cellular redox state through induction several antioxidant defence genes implicated in chemotherapeutic drugs resistance tumor cells. An increasing body evidence supports key role for kidney diseases and renal cell carcinoma (RCC), but data concerning molecular basis clinical effect its deregulation remain incomplete.Here we present profiling KEAP1 NFE2L2 five different Renal Cell Carcinoma histotypes by analysing 89 tumor/normal paired...
Abstract Purpose: EphA2 receptor is involved in multiple cross-talks with other cellular networks, including EGFR, FAK, and VEGF pathways, which it collaborates to stimulate cell migration, invasion, metastasis. Colorectal cancer (CRC) overexpression has also been correlated stem-like properties of cells tumor malignancy. We investigated the molecular cross-talk miRNAs modulation EGFR pathways. explored role EphA2/EGFR pathway mediators as prognostic factors or predictors cetuximab benefit...
Gliomas represent a disparate group of tumours for which there are to date no cure. Thus, is recognized need new diagnostic and therapeutic approaches based on increased understanding their molecular nature. We performed the comparison microRNA (miRNA) profile 8 WHO grade II gliomas 24 higher (2 III 22 glioblastomas) by using Affymetrix GeneChip miRNA Array v. 1.0. A relative quantification method (RT-qPCR) with standard curve was used confirm signature resulted array analysis. The...
Abstract Aberrant promoter methylation of several known or putative tumor suppressor genes occurs frequently during carcinogenesis, and this epigenetic change has been considered as a potential molecular marker for cancer. We examined the status nine (APC, CDH1, CTNNB1, TIMP3, ESR1, GSTP1, MGMT, THBS1, TMS1), by quantitative specific PCR. Synchronous preinvasive lesions (atypical ductal hyperplasia and/or carcinoma in situ) invasive breast from 52 patients, together with pure 24 patients 12...
Background: Plasmid DNA vaccination combined with electroporation (EP) provides a promising approach for the prevention of infectious diseases and cancer immunotherapy. This technology has been described as being effective in activating humoural cellular immune response host well enhancing expression encoded antigen. Several reports showed EP adjuvant-like properties when plasmid injection although effect absence not investigated. Objective: The aim this study is to clarify whether...
// Jianmin Wang 1,* , Antonios Papanicolau-Sengos 2,* Sreenivasulu Chintala 3,10,* Lei Wei 1 Biao Liu Qiang Hu Kiersten Marie Miles 2 Jeffrey M. Conroy Sean T. Glenn 4 Manuela Costantini 5,8,9 Cristina Magi-Galluzzi 6 Sabina Signoretti 7 Toni Choueiri Michele Gallucci 5 Steno Sentinelli Vito Fazio 8 Maria Luana Poeta 9 Song Carl Morrison and Roberto Pili 3,10 Department of Biostatistics & Bioinformatics, Roswell Park Cancer Institute, Buffalo, NY, USA Pathology Center for Personalized...
Abstract miR-9 was initially identified as an epigenetically regulated miRNA in tumours, but inconsistent findings have been reported so far. We analysed the expression of miR-9-5p, miR-9-3p, pri-miRs and MIR9 promoters methylation status 131 breast cancer cases 12 normal tissues (NBTs). The both mature miRs increased tumours compared to NBTs (P < 0.001) negatively correlated with ER protein ( P = 0.005 0.003, for miR-9-3p miR-9-5p respectively). In addition, showed a significant negative...