A5101561970- Sports, Gender, and Society
- Advanced NMR Techniques and Applications
- Sport and Mega-Event Impacts
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Recreation, Leisure, Wilderness Management
- Motivation and Self-Concept in Sports
- Cardiovascular and Diving-Related Complications
- Solid-state spectroscopy and crystallography
- Sports Analytics and Performance
- Anesthesia and Sedative Agents
- Electron Spin Resonance Studies
- Cardiac Arrest and Resuscitation
- Doping in Sports
- Cardiac, Anesthesia and Surgical Outcomes
- Ion channel regulation and function
- Athletic Training and Education
- Customer Service Quality and Loyalty
- Sports Performance and Training
- Physical Education and Pedagogy
- Ocean Acidification Effects and Responses
- Anesthesia and Neurotoxicity Research
- Thermal Regulation in Medicine
- NMR spectroscopy and applications
- Spaceflight effects on biology
Institut de Biologie Structurale
2011-2022
Bruker (Switzerland)
2022
Institut Pasteur de Lille
2022
Centre Hospitalier Universitaire de Lille
2022
Université de Lille
2016-2021
Unité de Glycobiologie Structurale et Fonctionnelle
2016-2021
Centre National de la Recherche Scientifique
2011-2021
Inserm
2015-2021
SUNY Brockport
2009-2019
Erciyes University
2019
Changes in regional blood flow during simulated normobaric diving were studied the conscious Antarctic Weddell seal (Leptonychotes weddelli) by injecting 25-microns radioactive microspheres into left ventricle. Injections performed before and 8--12 min after submersion of head iced seawater. Diving was associated with a fall cardiac output from mean control value 39.8 +/- 10.2 to 5.6 3.4 l/min (mean SD) heart rate 52 15 4 beats/min. Blood splanchnic peripheral vascular bed reduced more than...
Arterial blood gas tensions, pH, and hemoglobin concentrations were measured in four free-diving Weddell seals Leptonychotes weddelli. A microprocessor-controlled sampling system enabled us to obtain 24 single 31 serial aortic samples. The arterial O2 tension (PaO2) at rest [78 +/- 13 (SD) Torr] increased with diving compression a maximum value of 232 Torr then rapidly decreased 25-35 Torr. lowest PaO2 we was 18 just before the seal surfaced from 27-min dive. consistent increase 15.1 1.10...
Arterial blood nitrogen tensions of free-diving Weddell seals ( Leptonychotes weddelli ) were measured by attaching a microprocessor-controlled pump and drawing samples at depth to determine how these marine mammals dive great depths ascend rapidly without developing decompression sickness. Forty-seven arterial obtained from four during free dives lasting up 23 minutes 230 meters beneath the sea ice McMurdo Sound, Antarctica. Peak between 2000 2500 millimeters mercury recorded 40 80 descent,...
Despite playing important roles throughout biology, molecular recognition mechanisms in intrinsically disordered proteins remain poorly understood. We present a combination of (1)H(N), (13)C', and (15)N relaxation dispersion NMR, measured at multiple titration points, to map the interaction between domain Sendai virus nucleoprotein (NT) C-terminal phosphoprotein (PX). Interaction with PX funnels free-state equilibrium NT by stabilizing one previously identified helical substates...
Intrinsically disordered regions are predicted to exist in a significant fraction of proteins encoded eukaryotic genomes. The high levels conformational plasticity this class endows them with unique capacities act functional modes not achievable by folded proteins, but also places their molecular characterization beyond the reach classical structural biology. New techniques therefore required understand relationship between primary sequence and biological function proteins. Although...
The dynamic modes and time scales sampled by intrinsically disordered proteins (IDPs) define their function. Nuclear magnetic resonance (NMR) spin relaxation is probably the most powerful tool for investigating these motions delivering site-specific descriptions of conformational fluctuations from throughout molecule. Despite abundance experimental measurement in IDPs, physical origin measured rates remains poorly understood. Here we measure an extensive range auto- cross-correlated at...
Macrophages are diverse cell types in the first line of antimicrobial defense. Only a limited number primary mouse models exist to study their function. Bone marrow-derived, macrophage-CSF-induced cells with life span most common source. We report here simple method yielding self-renewing, nontransformed, GM-CSF/signal transducer and activator transcription 5-dependent macrophages (Max Planck Institute cells) from fetal liver, which reflect innate immune characteristics alveolar macrophages....
The spleen of the Weddell seal (Leptonychotes weddelli) may contract and inject red blood cells (RBCs) into peripheral circulation during diving, but evidence for this hypothesis is indirect. Accordingly, we measured splenic dimensions by ultrasonography, plasma catecholamine concentrations, hemoglobin concentration, hematocrit in five seals before after intravenous epinephrine halothane anesthesia while awake at surface voluntary dives. Spleen size was reduced immediately injection or...
Diazepam has been reported to produce hypotension when administered with anesthetic doses offentanyl. Twenty patients undergoing coronary bypass surgery were randomly assigned one of four treatment groups: group 1, no diazepam; groups 2, 3, and 4, 0.125, 0.25, 0.5 mg·kg−1 diazepam, respectively. All then received 50 μg·kg−1 fentanyl at 400 μg·min−1 0.4 metocurine 2 mg·min−1. Hemodynamic parameters recorded blood was sampled for measurement plasma catecholamine histamine concentrations. Heart...
We show that water-edited solid-state NMR spectroscopy allows for probing global protein conformation and residue-specific solvent accessibility in a lipid bilayer environment. The transfer dynamics can be well described by general time constant, irrespective of topology This approach was used to follow structural changes response function the chimeric potassium channel KcsA-Kv1.3. Data obtained as pH link earlier biochemical data structure functional setting.
Hendra virus (HeV) is a recently emerged severe human pathogen that belongs to the Henipavirus genus within Paramyxoviridae family. The HeV genome encapsidated by nucleoprotein (N) helical nucleocapsid. Recruitment of viral polymerase onto nucleocapsid template relies on interaction between C-terminal domain, N(TAIL), N and X XD, co-factor phosphoprotein (P). Here, we provide an atomic resolution description intrinsically disordered N(TAIL) domain in its isolated state intact nucleocapsids...